Monitoring the Spread of Multidrug-Resistant <i>Escherichia coli</i> Throughout the Broiler Production Cycle
The extensive use of antimicrobials in broiler production is changing the bird microbiota, fostering drug-resistant bacteria, and complicating therapeutic interventions, making the problem of multidrug resistance global. The monitoring of antimicrobial virulence and resistance genes are tools that h...
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2025-01-01
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| Series: | Antibiotics |
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| author | Victor Dellevedove Cruz Danilo Henrique Rabaçal Alves Jamile Kellen de Souza Maísa Fabiana Menck-Costa Bruno Henrique Dias de Oliva Ana Angelita Sampaio Baptista Alexandre Oba Fabrizio Matté Kácio Emílio Borges Baierle Sérgio Paulo Dejato da Rocha Kelly Cristina Tagliari de Brito Benito Guimarães de Brito Gerson Nakazato Marcio Costa Renata Katsuko Takayama Kobayashi |
| author_facet | Victor Dellevedove Cruz Danilo Henrique Rabaçal Alves Jamile Kellen de Souza Maísa Fabiana Menck-Costa Bruno Henrique Dias de Oliva Ana Angelita Sampaio Baptista Alexandre Oba Fabrizio Matté Kácio Emílio Borges Baierle Sérgio Paulo Dejato da Rocha Kelly Cristina Tagliari de Brito Benito Guimarães de Brito Gerson Nakazato Marcio Costa Renata Katsuko Takayama Kobayashi |
| author_sort | Victor Dellevedove Cruz |
| collection | DOAJ |
| description | The extensive use of antimicrobials in broiler production is changing the bird microbiota, fostering drug-resistant bacteria, and complicating therapeutic interventions, making the problem of multidrug resistance global. The monitoring of antimicrobial virulence and resistance genes are tools that have come to assist the breeding of these animals, directing possible treatments as already used in human medicine and collecting data to demonstrate possible dissemination of multidrug-resistant strains that may cause damage to industry and public health. This work aimed to monitor broiler farms in southern Brazil, isolating samples of <i>E. coli</i> and classifying them according to the profile of resistance to antimicrobials of interest to human and animal health. We also monitored the profile of virulence genes and conducted an epidemiological survey of possible risk factors that contribute to this selection of multidrug-resistant isolates. Monitoring was carried out on farms in the three southern states of the country, collecting samples of poultry litter, cloacal swabs, and beetles of the species <i>Alphitobius diaperinus</i>, isolating <i>E. coli</i> from each of these samples. These were evaluated by testing their susceptibility to antimicrobials of animal and human interest; detecting whether the samples were extended-spectrum β-lactamase enzyme (ESBL) producers; and when positive, selected for genotypic tests to identify resistant genes (CTX-M, TEM, and SHV) and virulence. Among the antimicrobials tested, enrofloxacin and ciprofloxacin demonstrated some of the highest frequencies of resistance in the isolated strains, with significant statistical results. The use of these antimicrobials increased the likelihood of resistance by over three times and was associated with a 1.5-fold higher probability of multidrug resistance. Of all isolates, 95% were multidrug-resistant, raising concerns for production and public health. Among 231 ESBL-positive samples, the CTX-M1 group predominated. |
| format | Article |
| id | doaj-art-dd5cffeb32f948ed879c873ca1619d04 |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Antibiotics |
| spelling | doaj-art-dd5cffeb32f948ed879c873ca1619d042025-01-24T13:18:50ZengMDPI AGAntibiotics2079-63822025-01-011416910.3390/antibiotics14010069Monitoring the Spread of Multidrug-Resistant <i>Escherichia coli</i> Throughout the Broiler Production CycleVictor Dellevedove Cruz0Danilo Henrique Rabaçal Alves1Jamile Kellen de Souza2Maísa Fabiana Menck-Costa3Bruno Henrique Dias de Oliva4Ana Angelita Sampaio Baptista5Alexandre Oba6Fabrizio Matté7Kácio Emílio Borges Baierle8Sérgio Paulo Dejato da Rocha9Kelly Cristina Tagliari de Brito10Benito Guimarães de Brito11Gerson Nakazato12Marcio Costa13Renata Katsuko Takayama Kobayashi14Department of Microbiology, Biological Sciences Center, State University of Londrina, Londrina 86057-970, BrazilDepartment of Microbiology, Biological Sciences Center, State University of Londrina, Londrina 86057-970, BrazilDepartment of Microbiology, Biological Sciences Center, State University of Londrina, Londrina 86057-970, BrazilDepartment of Microbiology, Biological Sciences Center, State University of Londrina, Londrina 86057-970, BrazilDepartment of Microbiology, Biological Sciences Center, State University of Londrina, Londrina 86057-970, BrazilAvian Medicine Laboratory, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina 86057-970, BrazilDepartment of Zootechnics, Poultry, Production and Quality of Broiler Meat, State University of Londrina, Londrina 86057-970, BrazilVetanco Brazil, Chapecó, Santa Catarina 89813-824, BrazilAvian Medicine Laboratory, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina 86057-970, BrazilDepartment of Microbiology, Biological Sciences Center, State University of Londrina, Londrina 86057-970, BrazilAvian Health Laboratory, Veterinary Research Institute Desidério Finamor, Agricultural Diagnosis and Research Department, Secretariat of Agriculture Livestock Rural and Development, Eldorado do Sul 90990-000, BrazilAvian Health Laboratory, Veterinary Research Institute Desidério Finamor, Agricultural Diagnosis and Research Department, Secretariat of Agriculture Livestock Rural and Development, Eldorado do Sul 90990-000, BrazilDepartment of Microbiology, Biological Sciences Center, State University of Londrina, Londrina 86057-970, BrazilDépartement de Biomédecine Vétérinaire, Faculté de Médecine Vétérinaire, Université de Montréal, Montreal, QC H3C 3J7, CanadaDepartment of Microbiology, Biological Sciences Center, State University of Londrina, Londrina 86057-970, BrazilThe extensive use of antimicrobials in broiler production is changing the bird microbiota, fostering drug-resistant bacteria, and complicating therapeutic interventions, making the problem of multidrug resistance global. The monitoring of antimicrobial virulence and resistance genes are tools that have come to assist the breeding of these animals, directing possible treatments as already used in human medicine and collecting data to demonstrate possible dissemination of multidrug-resistant strains that may cause damage to industry and public health. This work aimed to monitor broiler farms in southern Brazil, isolating samples of <i>E. coli</i> and classifying them according to the profile of resistance to antimicrobials of interest to human and animal health. We also monitored the profile of virulence genes and conducted an epidemiological survey of possible risk factors that contribute to this selection of multidrug-resistant isolates. Monitoring was carried out on farms in the three southern states of the country, collecting samples of poultry litter, cloacal swabs, and beetles of the species <i>Alphitobius diaperinus</i>, isolating <i>E. coli</i> from each of these samples. These were evaluated by testing their susceptibility to antimicrobials of animal and human interest; detecting whether the samples were extended-spectrum β-lactamase enzyme (ESBL) producers; and when positive, selected for genotypic tests to identify resistant genes (CTX-M, TEM, and SHV) and virulence. Among the antimicrobials tested, enrofloxacin and ciprofloxacin demonstrated some of the highest frequencies of resistance in the isolated strains, with significant statistical results. The use of these antimicrobials increased the likelihood of resistance by over three times and was associated with a 1.5-fold higher probability of multidrug resistance. Of all isolates, 95% were multidrug-resistant, raising concerns for production and public health. Among 231 ESBL-positive samples, the CTX-M1 group predominated.https://www.mdpi.com/2079-6382/14/1/69monitoringantimicrobial resistancevirulence genesone healthpoultry |
| spellingShingle | Victor Dellevedove Cruz Danilo Henrique Rabaçal Alves Jamile Kellen de Souza Maísa Fabiana Menck-Costa Bruno Henrique Dias de Oliva Ana Angelita Sampaio Baptista Alexandre Oba Fabrizio Matté Kácio Emílio Borges Baierle Sérgio Paulo Dejato da Rocha Kelly Cristina Tagliari de Brito Benito Guimarães de Brito Gerson Nakazato Marcio Costa Renata Katsuko Takayama Kobayashi Monitoring the Spread of Multidrug-Resistant <i>Escherichia coli</i> Throughout the Broiler Production Cycle Antibiotics monitoring antimicrobial resistance virulence genes one health poultry |
| title | Monitoring the Spread of Multidrug-Resistant <i>Escherichia coli</i> Throughout the Broiler Production Cycle |
| title_full | Monitoring the Spread of Multidrug-Resistant <i>Escherichia coli</i> Throughout the Broiler Production Cycle |
| title_fullStr | Monitoring the Spread of Multidrug-Resistant <i>Escherichia coli</i> Throughout the Broiler Production Cycle |
| title_full_unstemmed | Monitoring the Spread of Multidrug-Resistant <i>Escherichia coli</i> Throughout the Broiler Production Cycle |
| title_short | Monitoring the Spread of Multidrug-Resistant <i>Escherichia coli</i> Throughout the Broiler Production Cycle |
| title_sort | monitoring the spread of multidrug resistant i escherichia coli i throughout the broiler production cycle |
| topic | monitoring antimicrobial resistance virulence genes one health poultry |
| url | https://www.mdpi.com/2079-6382/14/1/69 |
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