Free DNA partially clarifies discrepancies between qPCR and the conventional phage quantification method.
To use phages in a personalized therapy and industrial applications, an accurate quantification is needed. The gold standard method, namely the culture-based double agar overlay (DAO) method, provides an accurate estimate of the number of infectious phages but is laborious and time-intensive. Quanti...
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| Main Authors: | Saar Van Overfelt, Hans Duyvejonck, Femke Baeke, Riet De Rycke, Maya Merabishvili, Stefan Vermeulen, Piet Cools, Mario Vaneechoutte, Els Van Mechelen |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2024-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0313774 |
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