MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies.
Mosquitoes transmit medically important human pathogens, including viruses like dengue virus and parasites such as Plasmodium spp., the causative agent of malaria. Mosquito microbiomes are critically important for the ability of mosquitoes to transmit disease-causing agents. However, while large col...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2024-11-01
|
| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.3002897 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850110288262594560 |
|---|---|
| author | Aidan Foo Laura E Brettell Holly L Nichols 2022 UW-Madison Capstone in Microbiology Students Miguel Medina Muñoz Jessica A Lysne Vishaal Dhokiya Ananya F Hoque Doug E Brackney Eric P Caragata Michael L Hutchinson Marcelo Jacobs-Lorena David J Lampe Edwige Martin Claire Valiente Moro Michael Povelones Sarah M Short Blaire Steven Jiannong Xu Timothy D Paustian Michelle R Rondon Grant L Hughes Kerri L Coon Eva Heinz |
| author_facet | Aidan Foo Laura E Brettell Holly L Nichols 2022 UW-Madison Capstone in Microbiology Students Miguel Medina Muñoz Jessica A Lysne Vishaal Dhokiya Ananya F Hoque Doug E Brackney Eric P Caragata Michael L Hutchinson Marcelo Jacobs-Lorena David J Lampe Edwige Martin Claire Valiente Moro Michael Povelones Sarah M Short Blaire Steven Jiannong Xu Timothy D Paustian Michelle R Rondon Grant L Hughes Kerri L Coon Eva Heinz |
| author_sort | Aidan Foo |
| collection | DOAJ |
| description | Mosquitoes transmit medically important human pathogens, including viruses like dengue virus and parasites such as Plasmodium spp., the causative agent of malaria. Mosquito microbiomes are critically important for the ability of mosquitoes to transmit disease-causing agents. However, while large collections of bacterial isolates and genomic data exist for vertebrate microbiomes, the vast majority of work in mosquitoes to date is based on 16S rRNA gene amplicon data that provides limited taxonomic resolution and no functional information. To address this gap and facilitate future studies using experimental microbiome manipulations, we generated a bacterial Mosquito-Associated Isolate Collection (MosAIC) consisting of 392 bacterial isolates with extensive metadata and high-quality draft genome assemblies that are publicly available, both isolates and sequence data, for use by the scientific community. MosAIC encompasses 142 species spanning 29 bacterial families, with members of the Enterobacteriaceae comprising 40% of the collection. Phylogenomic analysis of 3 genera, Enterobacter, Serratia, and Elizabethkingia, reveal lineages of mosquito-associated bacteria isolated from different mosquito species in multiple laboratories. Investigation into species' pangenomes further reveals clusters of genes specific to these lineages, which are of interest for future work to test for functions connected to mosquito host association. Altogether, we describe the generation of a physical collection of mosquito-associated bacterial isolates, their genomic data, and analyses of selected groups in context of genome data from closely related isolates, providing a unique, highly valuable resource for research on bacterial colonisation and adaptation within mosquito hosts. Future efforts will expand the collection to include broader geographic and host species representation, especially from individuals collected from field populations, as well as other mosquito-associated microbes, including fungi, archaea, and protozoa. |
| format | Article |
| id | doaj-art-dccd4b51fe7e44d1a2e4dec591992ea9 |
| institution | OA Journals |
| issn | 1544-9173 1545-7885 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj-art-dccd4b51fe7e44d1a2e4dec591992ea92025-08-20T02:37:51ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852024-11-012211e300289710.1371/journal.pbio.3002897MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies.Aidan FooLaura E BrettellHolly L Nichols2022 UW-Madison Capstone in Microbiology StudentsMiguel Medina MuñozJessica A LysneVishaal DhokiyaAnanya F HoqueDoug E BrackneyEric P CaragataMichael L HutchinsonMarcelo Jacobs-LorenaDavid J LampeEdwige MartinClaire Valiente MoroMichael PovelonesSarah M ShortBlaire StevenJiannong XuTimothy D PaustianMichelle R RondonGrant L HughesKerri L CoonEva HeinzMosquitoes transmit medically important human pathogens, including viruses like dengue virus and parasites such as Plasmodium spp., the causative agent of malaria. Mosquito microbiomes are critically important for the ability of mosquitoes to transmit disease-causing agents. However, while large collections of bacterial isolates and genomic data exist for vertebrate microbiomes, the vast majority of work in mosquitoes to date is based on 16S rRNA gene amplicon data that provides limited taxonomic resolution and no functional information. To address this gap and facilitate future studies using experimental microbiome manipulations, we generated a bacterial Mosquito-Associated Isolate Collection (MosAIC) consisting of 392 bacterial isolates with extensive metadata and high-quality draft genome assemblies that are publicly available, both isolates and sequence data, for use by the scientific community. MosAIC encompasses 142 species spanning 29 bacterial families, with members of the Enterobacteriaceae comprising 40% of the collection. Phylogenomic analysis of 3 genera, Enterobacter, Serratia, and Elizabethkingia, reveal lineages of mosquito-associated bacteria isolated from different mosquito species in multiple laboratories. Investigation into species' pangenomes further reveals clusters of genes specific to these lineages, which are of interest for future work to test for functions connected to mosquito host association. Altogether, we describe the generation of a physical collection of mosquito-associated bacterial isolates, their genomic data, and analyses of selected groups in context of genome data from closely related isolates, providing a unique, highly valuable resource for research on bacterial colonisation and adaptation within mosquito hosts. Future efforts will expand the collection to include broader geographic and host species representation, especially from individuals collected from field populations, as well as other mosquito-associated microbes, including fungi, archaea, and protozoa.https://doi.org/10.1371/journal.pbio.3002897 |
| spellingShingle | Aidan Foo Laura E Brettell Holly L Nichols 2022 UW-Madison Capstone in Microbiology Students Miguel Medina Muñoz Jessica A Lysne Vishaal Dhokiya Ananya F Hoque Doug E Brackney Eric P Caragata Michael L Hutchinson Marcelo Jacobs-Lorena David J Lampe Edwige Martin Claire Valiente Moro Michael Povelones Sarah M Short Blaire Steven Jiannong Xu Timothy D Paustian Michelle R Rondon Grant L Hughes Kerri L Coon Eva Heinz MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies. PLoS Biology |
| title | MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies. |
| title_full | MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies. |
| title_fullStr | MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies. |
| title_full_unstemmed | MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies. |
| title_short | MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies. |
| title_sort | mosaic an annotated collection of mosquito associated bacteria with high quality genome assemblies |
| url | https://doi.org/10.1371/journal.pbio.3002897 |
| work_keys_str_mv | AT aidanfoo mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT lauraebrettell mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT hollylnichols mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT 2022uwmadisoncapstoneinmicrobiologystudents mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT miguelmedinamunoz mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT jessicaalysne mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT vishaaldhokiya mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT ananyafhoque mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT dougebrackney mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT ericpcaragata mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT michaellhutchinson mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT marcelojacobslorena mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT davidjlampe mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT edwigemartin mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT clairevalientemoro mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT michaelpovelones mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT sarahmshort mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT blairesteven mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT jiannongxu mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT timothydpaustian mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT michellerrondon mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT grantlhughes mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT kerrilcoon mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies AT evaheinz mosaicanannotatedcollectionofmosquitoassociatedbacteriawithhighqualitygenomeassemblies |