Differential display of flower bud mRNA of genic male sterility (GMS) AB line in Chinese cabbage-pak-choi and analysis of differential cDNA fragments
The difference was compared by mRNA differential display technology in Chinese cabbage-pak-choi (Brassica campestris ssp. chinensis Makino var. communis Tsen et Lee) flower bud among genic male sterility (GMS) line (A line) and maintainer line (B line). 18 differential fragments have been obtained i...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2001-11-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2001.06.0596 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849247119105327104 |
|---|---|
| author | CAO Jia-shu YE Wan-zhi ZHANG Ming ZENG Guang-wen |
| author_facet | CAO Jia-shu YE Wan-zhi ZHANG Ming ZENG Guang-wen |
| author_sort | CAO Jia-shu |
| collection | DOAJ |
| description | The difference was compared by mRNA differential display technology in Chinese cabbage-pak-choi (Brassica campestris ssp. chinensis Makino var. communis Tsen et Lee) flower bud among genic male sterility (GMS) line (A line) and maintainer line (B line). 18 differential fragments have been obtained in total of 120 selective amplifications. A differential fragment named as B0302-4 was confirmed by Northern hybridization out of 6 cDNA differential fragments tested. B0302-4 was shared by the maintainer line the expression of B0302-4 was restricted in the GMS line, and B0302 4 expressed specially in flower bud. Fragment B0302-4 is composed of 463 base pair. The result of Gen-Bank BLAST showed that it has 84.3% nucleotide similarity to the CYP86C4 gene encoding cytochrome P450 from Arubidopsis. |
| format | Article |
| id | doaj-art-dcab85eda42e43d088315aac963bd2a2 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2001-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-dcab85eda42e43d088315aac963bd2a22025-08-20T03:58:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552001-11-012759660010.3785/1008-9209.2001.06.059610089209Differential display of flower bud mRNA of genic male sterility (GMS) AB line in Chinese cabbage-pak-choi and analysis of differential cDNA fragmentsCAO Jia-shuYE Wan-zhiZHANG MingZENG Guang-wenThe difference was compared by mRNA differential display technology in Chinese cabbage-pak-choi (Brassica campestris ssp. chinensis Makino var. communis Tsen et Lee) flower bud among genic male sterility (GMS) line (A line) and maintainer line (B line). 18 differential fragments have been obtained in total of 120 selective amplifications. A differential fragment named as B0302-4 was confirmed by Northern hybridization out of 6 cDNA differential fragments tested. B0302-4 was shared by the maintainer line the expression of B0302-4 was restricted in the GMS line, and B0302 4 expressed specially in flower bud. Fragment B0302-4 is composed of 463 base pair. The result of Gen-Bank BLAST showed that it has 84.3% nucleotide similarity to the CYP86C4 gene encoding cytochrome P450 from Arubidopsis.https://www.academax.com/doi/10.3785/1008-9209.2001.06.0596Chinese cabbage-pak-choi（<italic>Brassica campestris</italic> ssp. <italic>chinensis</italic> Makino var. <italic>communis</italic> Tsen et Lee）genic male sterilitymRNA differential display |
| spellingShingle | CAO Jia-shu YE Wan-zhi ZHANG Ming ZENG Guang-wen Differential display of flower bud mRNA of genic male sterility (GMS) AB line in Chinese cabbage-pak-choi and analysis of differential cDNA fragments 浙江大学学报. 农业与生命科学版 Chinese cabbage-pak-choi（<italic>Brassica campestris</italic> ssp. <italic>chinensis</italic> Makino var. <italic>communis</italic> Tsen et Lee） genic male sterility mRNA differential display |
| title | Differential display of flower bud mRNA of genic male sterility (GMS) AB line in Chinese cabbage-pak-choi and analysis of differential cDNA fragments |
| title_full | Differential display of flower bud mRNA of genic male sterility (GMS) AB line in Chinese cabbage-pak-choi and analysis of differential cDNA fragments |
| title_fullStr | Differential display of flower bud mRNA of genic male sterility (GMS) AB line in Chinese cabbage-pak-choi and analysis of differential cDNA fragments |
| title_full_unstemmed | Differential display of flower bud mRNA of genic male sterility (GMS) AB line in Chinese cabbage-pak-choi and analysis of differential cDNA fragments |
| title_short | Differential display of flower bud mRNA of genic male sterility (GMS) AB line in Chinese cabbage-pak-choi and analysis of differential cDNA fragments |
| title_sort | differential display of flower bud mrna of genic male sterility gms ab line in chinese cabbage pak choi and analysis of differential cdna fragments |
| topic | Chinese cabbage-pak-choi（<italic>Brassica campestris</italic> ssp. <italic>chinensis</italic> Makino var. <italic>communis</italic> Tsen et Lee） genic male sterility mRNA differential display |
| url | https://www.academax.com/doi/10.3785/1008-9209.2001.06.0596 |
| work_keys_str_mv | AT caojiashu differentialdisplayofflowerbudmrnaofgenicmalesterilitygmsablineinchinesecabbagepakchoiandanalysisofdifferentialcdnafragments AT yewanzhi differentialdisplayofflowerbudmrnaofgenicmalesterilitygmsablineinchinesecabbagepakchoiandanalysisofdifferentialcdnafragments AT zhangming differentialdisplayofflowerbudmrnaofgenicmalesterilitygmsablineinchinesecabbagepakchoiandanalysisofdifferentialcdnafragments AT zengguangwen differentialdisplayofflowerbudmrnaofgenicmalesterilitygmsablineinchinesecabbagepakchoiandanalysisofdifferentialcdnafragments |