Potent Antioxidative and UVB Protective Effect of Water Extract of Eclipta prostrata L.
Oxidative stress, including Ultraviolet (UV) irradiation-induced skin damage, is involved in numerous diseases. This study demonstrates that water extract of Eclipta prostrata L. (WEP) has a potent effect in scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radicals, and chelating ferrous...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/759039 |
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| author | Chin-Feng Chan Wen-Ying Huang Hong-Yi Guo Bo Rong Wang |
| author_facet | Chin-Feng Chan Wen-Ying Huang Hong-Yi Guo Bo Rong Wang |
| author_sort | Chin-Feng Chan |
| collection | DOAJ |
| description | Oxidative stress, including Ultraviolet (UV) irradiation-induced skin damage, is involved in numerous diseases. This study demonstrates that water extract of Eclipta prostrata L. (WEP) has a potent effect in scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radicals, and chelating ferrous ion, exhibiting IC50 values of 0.23 mg/mL, 0.48 mg/mL, and 1.25 mg/mL, respectively. The WEP total phenol content was 176.45 mg gallic acid equivalents (GAE)/g sample. Chlorogenic acid, a component of the plant's active ingredients, was determined by HPLC and antioxidative assay. However, no caffeic acid, stigmasterol, or wedelolactone was present in WEP. WEP absorbs both UVA and UVB irradiation, and furthermore, the extract shows a dose-dependent response in the protection of HaCaT human keratinocytes and mouse fibroblasts 3T3 cells against UVB-induced cytotoxicity, which may result from a synergistic effect between chlorogenic acid and other active components present in WEP. |
| format | Article |
| id | doaj-art-dc514bd2e2a646eab7f9d77af02e8920 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-dc514bd2e2a646eab7f9d77af02e89202025-02-03T06:12:14ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/759039759039Potent Antioxidative and UVB Protective Effect of Water Extract of Eclipta prostrata L.Chin-Feng Chan0Wen-Ying Huang1Hong-Yi Guo2Bo Rong Wang3Department of Applied Cosmetology, Hungkuang University, Taichung 43302, TaiwanDepartment of Applied Cosmetology, Hungkuang University, Taichung 43302, TaiwanDepartment of Applied Cosmetology, Hungkuang University, Taichung 43302, TaiwanDepartment of Applied Cosmetology, Hungkuang University, Taichung 43302, TaiwanOxidative stress, including Ultraviolet (UV) irradiation-induced skin damage, is involved in numerous diseases. This study demonstrates that water extract of Eclipta prostrata L. (WEP) has a potent effect in scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radicals, and chelating ferrous ion, exhibiting IC50 values of 0.23 mg/mL, 0.48 mg/mL, and 1.25 mg/mL, respectively. The WEP total phenol content was 176.45 mg gallic acid equivalents (GAE)/g sample. Chlorogenic acid, a component of the plant's active ingredients, was determined by HPLC and antioxidative assay. However, no caffeic acid, stigmasterol, or wedelolactone was present in WEP. WEP absorbs both UVA and UVB irradiation, and furthermore, the extract shows a dose-dependent response in the protection of HaCaT human keratinocytes and mouse fibroblasts 3T3 cells against UVB-induced cytotoxicity, which may result from a synergistic effect between chlorogenic acid and other active components present in WEP.http://dx.doi.org/10.1155/2014/759039 |
| spellingShingle | Chin-Feng Chan Wen-Ying Huang Hong-Yi Guo Bo Rong Wang Potent Antioxidative and UVB Protective Effect of Water Extract of Eclipta prostrata L. The Scientific World Journal |
| title | Potent Antioxidative and UVB Protective Effect of Water Extract of Eclipta prostrata L. |
| title_full | Potent Antioxidative and UVB Protective Effect of Water Extract of Eclipta prostrata L. |
| title_fullStr | Potent Antioxidative and UVB Protective Effect of Water Extract of Eclipta prostrata L. |
| title_full_unstemmed | Potent Antioxidative and UVB Protective Effect of Water Extract of Eclipta prostrata L. |
| title_short | Potent Antioxidative and UVB Protective Effect of Water Extract of Eclipta prostrata L. |
| title_sort | potent antioxidative and uvb protective effect of water extract of eclipta prostrata l |
| url | http://dx.doi.org/10.1155/2014/759039 |
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