Determination of Scopolamine Distribution in Plasma and Brain by LC-MS/MS in Rats
Scopolamine, as a tropane alkaloid found in plants such as belladonna and datura, is used clinically as a transdermal patch and is highly neurotoxic. This study aimed to develop a simple, sensitive, and selective LC-MS/MS method for the determination of the content and distribution of scopolamine in...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/8536235 |
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| author | Juan Chen Anjing Lu Daopeng Tan Qianru Zhang Yanliu Lu Lin Qin Yuqi He |
| author_facet | Juan Chen Anjing Lu Daopeng Tan Qianru Zhang Yanliu Lu Lin Qin Yuqi He |
| author_sort | Juan Chen |
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| description | Scopolamine, as a tropane alkaloid found in plants such as belladonna and datura, is used clinically as a transdermal patch and is highly neurotoxic. This study aimed to develop a simple, sensitive, and selective LC-MS/MS method for the determination of the content and distribution of scopolamine in rat plasma and brain after drug administration. In our study, sample pretreatment consisted of protein precipitation with acetonitrile followed by nitrogen blow concentration. Gradient elution of scopolamine and internal standard was performed on a ZORBAX Eclipse Plus C18 (2.1∗100 mm, 3.5 μm) column with water containing 0.1% formic acid (v/v) and acetonitrile as a mobile phase. Those samples were quantified in ESI positive ion mode using an API 4000 triple quadrupole mass spectrometer. The results showed that scopolamine was linear in the calibration range of 2–2500 ng/mL, and the selectivity, accuracy, precision, matrix effect, stability, and recovery of the method were within acceptable limits. The method has been validated and has been successfully used for toxicokinetic studies of scopolamine. After intraperitoneal injection, the time to peak toxic concentrations of scopolamine in rats was 0.5 h. The concentrations of scopolamine in the hippocampus and cortex were much higher than those in the striatum, indicating that the likely targets of its neurotoxic damage were the hippocampus and cortex. Overall, this study provides the basis for the neurotoxicity of scopolamine and provides a reference for its toxicokinetic studies. |
| format | Article |
| id | doaj-art-dc4ac59631ef4bd287b465342e6ef541 |
| institution | Kabale University |
| issn | 1687-8779 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-dc4ac59631ef4bd287b465342e6ef5412025-02-03T01:22:53ZengWileyInternational Journal of Analytical Chemistry1687-87792022-01-01202210.1155/2022/8536235Determination of Scopolamine Distribution in Plasma and Brain by LC-MS/MS in RatsJuan Chen0Anjing Lu1Daopeng Tan2Qianru Zhang3Yanliu Lu4Lin Qin5Yuqi He6Guizhou Engineering Research Center of Industrial Key-technology for Dendrobium NobileGuizhou Engineering Research Center of Industrial Key-technology for Dendrobium NobileGuizhou Engineering Research Center of Industrial Key-technology for Dendrobium NobileGuizhou Engineering Research Center of Industrial Key-technology for Dendrobium NobileGuizhou Engineering Research Center of Industrial Key-technology for Dendrobium NobileGuizhou Engineering Research Center of Industrial Key-technology for Dendrobium NobileGuizhou Engineering Research Center of Industrial Key-technology for Dendrobium NobileScopolamine, as a tropane alkaloid found in plants such as belladonna and datura, is used clinically as a transdermal patch and is highly neurotoxic. This study aimed to develop a simple, sensitive, and selective LC-MS/MS method for the determination of the content and distribution of scopolamine in rat plasma and brain after drug administration. In our study, sample pretreatment consisted of protein precipitation with acetonitrile followed by nitrogen blow concentration. Gradient elution of scopolamine and internal standard was performed on a ZORBAX Eclipse Plus C18 (2.1∗100 mm, 3.5 μm) column with water containing 0.1% formic acid (v/v) and acetonitrile as a mobile phase. Those samples were quantified in ESI positive ion mode using an API 4000 triple quadrupole mass spectrometer. The results showed that scopolamine was linear in the calibration range of 2–2500 ng/mL, and the selectivity, accuracy, precision, matrix effect, stability, and recovery of the method were within acceptable limits. The method has been validated and has been successfully used for toxicokinetic studies of scopolamine. After intraperitoneal injection, the time to peak toxic concentrations of scopolamine in rats was 0.5 h. The concentrations of scopolamine in the hippocampus and cortex were much higher than those in the striatum, indicating that the likely targets of its neurotoxic damage were the hippocampus and cortex. Overall, this study provides the basis for the neurotoxicity of scopolamine and provides a reference for its toxicokinetic studies.http://dx.doi.org/10.1155/2022/8536235 |
| spellingShingle | Juan Chen Anjing Lu Daopeng Tan Qianru Zhang Yanliu Lu Lin Qin Yuqi He Determination of Scopolamine Distribution in Plasma and Brain by LC-MS/MS in Rats International Journal of Analytical Chemistry |
| title | Determination of Scopolamine Distribution in Plasma and Brain by LC-MS/MS in Rats |
| title_full | Determination of Scopolamine Distribution in Plasma and Brain by LC-MS/MS in Rats |
| title_fullStr | Determination of Scopolamine Distribution in Plasma and Brain by LC-MS/MS in Rats |
| title_full_unstemmed | Determination of Scopolamine Distribution in Plasma and Brain by LC-MS/MS in Rats |
| title_short | Determination of Scopolamine Distribution in Plasma and Brain by LC-MS/MS in Rats |
| title_sort | determination of scopolamine distribution in plasma and brain by lc ms ms in rats |
| url | http://dx.doi.org/10.1155/2022/8536235 |
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