Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect
Osteomyelitis is an infectious disease which is also a major complication of bone defects. This study aims to determine the effect of bovine hydroxyapatite-gelatin-based bone implants with gentamicin as an antibiotic (BHA-GEL-GEN implant) on the regeneration of bone defects in vivo. The BHA-GEL-GEN...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2021/5560891 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563249561731072 |
|---|---|
| author | Aniek S. Budiatin Maria A. Gani Samirah Chrismawan Ardianto Aulia M. Raharjanti Indah Septiani Ni Putu K. P. Putri Junaidi Khotib |
| author_facet | Aniek S. Budiatin Maria A. Gani Samirah Chrismawan Ardianto Aulia M. Raharjanti Indah Septiani Ni Putu K. P. Putri Junaidi Khotib |
| author_sort | Aniek S. Budiatin |
| collection | DOAJ |
| description | Osteomyelitis is an infectious disease which is also a major complication of bone defects. This study aims to determine the effect of bovine hydroxyapatite-gelatin-based bone implants with gentamicin as an antibiotic (BHA-GEL-GEN implant) on the regeneration of bone defects in vivo. The BHA-GEL-GEN and BHA-GEL implants were made by direct compression. In vivo study was carried out with Wistar rats. The rats were divided into three groups: negative control, BHA-GEL implant, and BHA-GEL-GEN implants. The defect model used was the burr hole defect model with diameter 2.2 mm and 2 mm deep. After 2, 7, 14, and 28 days, the rats were sacrificed. Bone integrity was carried out using X-ray radiography. Radiological examination was performed using haematoxylin and eosin (HE) staining and immunohistochemical techniques with anti-vascular endothelial growth factor (VEGF) and anti-alkaline phosphatase (ALP) antibodies. Based on the radiograph, the implanted group had accelerated bone growth in the defect area. Semiquantitative data from HE staining showed that the implanted group had accelerated migration of osteoclasts, osteoblasts, and osteocytes in the defect area. The immunoreactive score showed that the BHA-GEL-GEN group had higher VEGF expression compared to two other groups. The three groups did not provide a significant difference in ALP expression. In conclusion, the BHA-GEL-GEN implant causes accelerated bone defects repair by accelerating tissue vascularity and does not interfere with the bone remodeling process. Therefore, the BHA-GEL-GEN implant is potentially a biomedical material for osteomyelitis therapy. |
| format | Article |
| id | doaj-art-dc30dcfa25ed4f69b89c6638e257b0f4 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-dc30dcfa25ed4f69b89c6638e257b0f42025-02-03T01:20:44ZengWileyInternational Journal of Biomaterials1687-87871687-87952021-01-01202110.1155/2021/55608915560891Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone DefectAniek S. Budiatin0Maria A. Gani1Samirah2Chrismawan Ardianto3Aulia M. Raharjanti4Indah Septiani5Ni Putu K. P. Putri6Junaidi Khotib7Department of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Surabaya, IndonesiaDoctoral Programme of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, IndonesiaDepartment of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Surabaya, IndonesiaDepartment of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Surabaya, IndonesiaDepartment of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Surabaya, IndonesiaDepartment of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Surabaya, IndonesiaDepartment of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Surabaya, IndonesiaDepartment of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Surabaya, IndonesiaOsteomyelitis is an infectious disease which is also a major complication of bone defects. This study aims to determine the effect of bovine hydroxyapatite-gelatin-based bone implants with gentamicin as an antibiotic (BHA-GEL-GEN implant) on the regeneration of bone defects in vivo. The BHA-GEL-GEN and BHA-GEL implants were made by direct compression. In vivo study was carried out with Wistar rats. The rats were divided into three groups: negative control, BHA-GEL implant, and BHA-GEL-GEN implants. The defect model used was the burr hole defect model with diameter 2.2 mm and 2 mm deep. After 2, 7, 14, and 28 days, the rats were sacrificed. Bone integrity was carried out using X-ray radiography. Radiological examination was performed using haematoxylin and eosin (HE) staining and immunohistochemical techniques with anti-vascular endothelial growth factor (VEGF) and anti-alkaline phosphatase (ALP) antibodies. Based on the radiograph, the implanted group had accelerated bone growth in the defect area. Semiquantitative data from HE staining showed that the implanted group had accelerated migration of osteoclasts, osteoblasts, and osteocytes in the defect area. The immunoreactive score showed that the BHA-GEL-GEN group had higher VEGF expression compared to two other groups. The three groups did not provide a significant difference in ALP expression. In conclusion, the BHA-GEL-GEN implant causes accelerated bone defects repair by accelerating tissue vascularity and does not interfere with the bone remodeling process. Therefore, the BHA-GEL-GEN implant is potentially a biomedical material for osteomyelitis therapy.http://dx.doi.org/10.1155/2021/5560891 |
| spellingShingle | Aniek S. Budiatin Maria A. Gani Samirah Chrismawan Ardianto Aulia M. Raharjanti Indah Septiani Ni Putu K. P. Putri Junaidi Khotib Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect International Journal of Biomaterials |
| title | Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect |
| title_full | Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect |
| title_fullStr | Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect |
| title_full_unstemmed | Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect |
| title_short | Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect |
| title_sort | bovine hydroxyapatite based bone scaffold with gentamicin accelerates vascularization and remodeling of bone defect |
| url | http://dx.doi.org/10.1155/2021/5560891 |
| work_keys_str_mv | AT anieksbudiatin bovinehydroxyapatitebasedbonescaffoldwithgentamicinacceleratesvascularizationandremodelingofbonedefect AT mariaagani bovinehydroxyapatitebasedbonescaffoldwithgentamicinacceleratesvascularizationandremodelingofbonedefect AT samirah bovinehydroxyapatitebasedbonescaffoldwithgentamicinacceleratesvascularizationandremodelingofbonedefect AT chrismawanardianto bovinehydroxyapatitebasedbonescaffoldwithgentamicinacceleratesvascularizationandremodelingofbonedefect AT auliamraharjanti bovinehydroxyapatitebasedbonescaffoldwithgentamicinacceleratesvascularizationandremodelingofbonedefect AT indahseptiani bovinehydroxyapatitebasedbonescaffoldwithgentamicinacceleratesvascularizationandremodelingofbonedefect AT niputukpputri bovinehydroxyapatitebasedbonescaffoldwithgentamicinacceleratesvascularizationandremodelingofbonedefect AT junaidikhotib bovinehydroxyapatitebasedbonescaffoldwithgentamicinacceleratesvascularizationandremodelingofbonedefect |