Dietary Alpha-Lipoic Acid Alleviated Hepatic Glycogen Deposition and Improved Inflammation Response of Largemouth Bass (<i>Micropterus salmoides</i>) Fed on High Dietary Carbohydrates
In order to mitigate the adverse effects of high carbohydrates on largemouth bass and to investigate the feasibility of LA as a feed additive, the present study observed the effects of added α-lipoic acid (LA) on growth performance, glucose metabolism and immunity in largemouth bass fed on high diet...
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2024-12-01
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| author | Zishuo Fang Xianwei Pan Ye Gong Nihe Zhang Shiwen Chen Ning Liu Naisong Chen Songlin Li |
| author_facet | Zishuo Fang Xianwei Pan Ye Gong Nihe Zhang Shiwen Chen Ning Liu Naisong Chen Songlin Li |
| author_sort | Zishuo Fang |
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| description | In order to mitigate the adverse effects of high carbohydrates on largemouth bass and to investigate the feasibility of LA as a feed additive, the present study observed the effects of added α-lipoic acid (LA) on growth performance, glucose metabolism and immunity in largemouth bass fed on high dietary carbohydrates (10% α-cassava starch inclusion). A total of 315 juvenile largemouth bass (initial body weight, 5.09 ± 0.10 g) were divided into nine tanks (800 L) (upper radius 0.65 m × lower radius 0.5 m × height 1 m), with each holding 35 fish. Three iso-nitrogenous and iso-lipidic diets supplementing with 0 g/kg, 0.5 g/kg and 1 g/kg LA (LA0, LA500, LA1000) were designed to feed juvenile largemouth bass on a satiation diet twice daily for eight weeks with each diet feeding to triplicate groups. The results indicated that the performance in growth was significantly enhanced by the addition of dietary LA (<i>p</i> < 0.05). Meanwhile, hepatic glycogen content was significantly reduced (<i>p</i> < 0.05), and the expression of genes relating to insulin pathway and glycolysis significantly increased with LA inclusion (<i>p</i> < 0.05). The relative expression of insulin receptor a (<i>ira</i>) in the LA500 group was the highest, while the relative expression of glycerol kinase (<i>gk</i>), phosphofructokinase liver type (<i>pfkl</i>) and phosphoenolpyruvate carboxykinase (<i>pepck</i>) was the highest in the LA1000 group (<i>p</i> < 0.05). In addition, LA supplementation significantly increased the activity of lysozyme, which reached its maximum value in the LA500 group (<i>p</i> < 0.05). LA supplementation also promoted the expression of genes relating to anti-inflammatory and inhibited the expression of pro-inflammatory related genes (<i>p</i> < 0.05). Above all, the dietary addition of LA could improve performance in growth, alleviated hepatic glycogen deposition, and improved the immunity function of largemouth bass fed on high dietary carbohydrates. This provides us with ideas to mitigate the adverse effects of high carbohydrates on largemouth bass in actual production and provides a basis for the application of LA in aquatic biology. |
| format | Article |
| id | doaj-art-dc2d744eeba4490aa936890930b69f5c |
| institution | Kabale University |
| issn | 2410-3888 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Fishes |
| spelling | doaj-art-dc2d744eeba4490aa936890930b69f5c2025-01-24T13:32:25ZengMDPI AGFishes2410-38882024-12-01101910.3390/fishes10010009Dietary Alpha-Lipoic Acid Alleviated Hepatic Glycogen Deposition and Improved Inflammation Response of Largemouth Bass (<i>Micropterus salmoides</i>) Fed on High Dietary CarbohydratesZishuo Fang0Xianwei Pan1Ye Gong2Nihe Zhang3Shiwen Chen4Ning Liu5Naisong Chen6Songlin Li7Research Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai 201306, ChinaInternational Research Centre for Food and Health, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaResearch Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai 201306, ChinaResearch Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai 201306, ChinaResearch Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai 201306, ChinaInternational Research Centre for Food and Health, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaResearch Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai 201306, ChinaResearch Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai 201306, ChinaIn order to mitigate the adverse effects of high carbohydrates on largemouth bass and to investigate the feasibility of LA as a feed additive, the present study observed the effects of added α-lipoic acid (LA) on growth performance, glucose metabolism and immunity in largemouth bass fed on high dietary carbohydrates (10% α-cassava starch inclusion). A total of 315 juvenile largemouth bass (initial body weight, 5.09 ± 0.10 g) were divided into nine tanks (800 L) (upper radius 0.65 m × lower radius 0.5 m × height 1 m), with each holding 35 fish. Three iso-nitrogenous and iso-lipidic diets supplementing with 0 g/kg, 0.5 g/kg and 1 g/kg LA (LA0, LA500, LA1000) were designed to feed juvenile largemouth bass on a satiation diet twice daily for eight weeks with each diet feeding to triplicate groups. The results indicated that the performance in growth was significantly enhanced by the addition of dietary LA (<i>p</i> < 0.05). Meanwhile, hepatic glycogen content was significantly reduced (<i>p</i> < 0.05), and the expression of genes relating to insulin pathway and glycolysis significantly increased with LA inclusion (<i>p</i> < 0.05). The relative expression of insulin receptor a (<i>ira</i>) in the LA500 group was the highest, while the relative expression of glycerol kinase (<i>gk</i>), phosphofructokinase liver type (<i>pfkl</i>) and phosphoenolpyruvate carboxykinase (<i>pepck</i>) was the highest in the LA1000 group (<i>p</i> < 0.05). In addition, LA supplementation significantly increased the activity of lysozyme, which reached its maximum value in the LA500 group (<i>p</i> < 0.05). LA supplementation also promoted the expression of genes relating to anti-inflammatory and inhibited the expression of pro-inflammatory related genes (<i>p</i> < 0.05). Above all, the dietary addition of LA could improve performance in growth, alleviated hepatic glycogen deposition, and improved the immunity function of largemouth bass fed on high dietary carbohydrates. This provides us with ideas to mitigate the adverse effects of high carbohydrates on largemouth bass in actual production and provides a basis for the application of LA in aquatic biology.https://www.mdpi.com/2410-3888/10/1/9α-lipoic acidglycogen depositionimmunity functionlargemouth bass |
| spellingShingle | Zishuo Fang Xianwei Pan Ye Gong Nihe Zhang Shiwen Chen Ning Liu Naisong Chen Songlin Li Dietary Alpha-Lipoic Acid Alleviated Hepatic Glycogen Deposition and Improved Inflammation Response of Largemouth Bass (<i>Micropterus salmoides</i>) Fed on High Dietary Carbohydrates Fishes α-lipoic acid glycogen deposition immunity function largemouth bass |
| title | Dietary Alpha-Lipoic Acid Alleviated Hepatic Glycogen Deposition and Improved Inflammation Response of Largemouth Bass (<i>Micropterus salmoides</i>) Fed on High Dietary Carbohydrates |
| title_full | Dietary Alpha-Lipoic Acid Alleviated Hepatic Glycogen Deposition and Improved Inflammation Response of Largemouth Bass (<i>Micropterus salmoides</i>) Fed on High Dietary Carbohydrates |
| title_fullStr | Dietary Alpha-Lipoic Acid Alleviated Hepatic Glycogen Deposition and Improved Inflammation Response of Largemouth Bass (<i>Micropterus salmoides</i>) Fed on High Dietary Carbohydrates |
| title_full_unstemmed | Dietary Alpha-Lipoic Acid Alleviated Hepatic Glycogen Deposition and Improved Inflammation Response of Largemouth Bass (<i>Micropterus salmoides</i>) Fed on High Dietary Carbohydrates |
| title_short | Dietary Alpha-Lipoic Acid Alleviated Hepatic Glycogen Deposition and Improved Inflammation Response of Largemouth Bass (<i>Micropterus salmoides</i>) Fed on High Dietary Carbohydrates |
| title_sort | dietary alpha lipoic acid alleviated hepatic glycogen deposition and improved inflammation response of largemouth bass i micropterus salmoides i fed on high dietary carbohydrates |
| topic | α-lipoic acid glycogen deposition immunity function largemouth bass |
| url | https://www.mdpi.com/2410-3888/10/1/9 |
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