Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin
Visible-range circular dichroism titrations were used to study Cu(II) binding properties of Multimetal Binding Site (MBS) of Human Serum Albumin (HSA). The formation of ternary MBS-Cu(II)-Buffer complexes at pH 7.4 was positively verified for sodium phosphate, Tris, and Hepes, the three most common...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2010/725153 |
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| author | Magdalena Sokołowska Krystyna Pawlas Wojciech Bal |
| author_facet | Magdalena Sokołowska Krystyna Pawlas Wojciech Bal |
| author_sort | Magdalena Sokołowska |
| collection | DOAJ |
| description | Visible-range circular dichroism titrations were used to study Cu(II) binding properties of Multimetal Binding Site (MBS) of Human Serum Albumin (HSA). The formation of ternary MBS-Cu(II)-Buffer complexes at pH 7.4 was positively verified for sodium phosphate, Tris, and Hepes, the three most common biochemical buffers. The phosphate > Hepes > Tris order of affinities, together with strong spectral changes induced specifically by Tris, indicates the presence of both Buffer-Cu(II) and Buffer-HSA interactions. All complexes are strong enough to yield a nearly 100% ternary complex formation in 0.5 mM HSA dissolved in 100 mM solutions of respective buffers. The effects of warfarin and ibuprofen, specific ligands of hydrophobic pockets I and II in HSA on the Cu(II) binding to MBS were also investigated. The effects of ibuprofen were negligible, but warfarin diminished the MBS affinity for Cu(II) by a factor of 20, as a result of indirect conformational effects. These results indicate that metal binding properties of MBS can be modulated directly and indirectly by small molecules. |
| format | Article |
| id | doaj-art-dc156fb3488e450287a683f64e3c1cc4 |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-dc156fb3488e450287a683f64e3c1cc42025-02-03T01:24:30ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2010-01-01201010.1155/2010/725153725153Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum AlbuminMagdalena Sokołowska0Krystyna Pawlas1Wojciech Bal2Department of Hygiene, Wrocław Medical University, Mikulicza-Radeckiego 7, 50-345 Wrocław, PolandDepartment of Hygiene, Wrocław Medical University, Mikulicza-Radeckiego 7, 50-345 Wrocław, PolandInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, PolandVisible-range circular dichroism titrations were used to study Cu(II) binding properties of Multimetal Binding Site (MBS) of Human Serum Albumin (HSA). The formation of ternary MBS-Cu(II)-Buffer complexes at pH 7.4 was positively verified for sodium phosphate, Tris, and Hepes, the three most common biochemical buffers. The phosphate > Hepes > Tris order of affinities, together with strong spectral changes induced specifically by Tris, indicates the presence of both Buffer-Cu(II) and Buffer-HSA interactions. All complexes are strong enough to yield a nearly 100% ternary complex formation in 0.5 mM HSA dissolved in 100 mM solutions of respective buffers. The effects of warfarin and ibuprofen, specific ligands of hydrophobic pockets I and II in HSA on the Cu(II) binding to MBS were also investigated. The effects of ibuprofen were negligible, but warfarin diminished the MBS affinity for Cu(II) by a factor of 20, as a result of indirect conformational effects. These results indicate that metal binding properties of MBS can be modulated directly and indirectly by small molecules.http://dx.doi.org/10.1155/2010/725153 |
| spellingShingle | Magdalena Sokołowska Krystyna Pawlas Wojciech Bal Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin Bioinorganic Chemistry and Applications |
| title | Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin |
| title_full | Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin |
| title_fullStr | Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin |
| title_full_unstemmed | Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin |
| title_short | Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin |
| title_sort | effect of common buffers and heterocyclic ligands on the binding of cu ii at the multimetal binding site in human serum albumin |
| url | http://dx.doi.org/10.1155/2010/725153 |
| work_keys_str_mv | AT magdalenasokołowska effectofcommonbuffersandheterocyclicligandsonthebindingofcuiiatthemultimetalbindingsiteinhumanserumalbumin AT krystynapawlas effectofcommonbuffersandheterocyclicligandsonthebindingofcuiiatthemultimetalbindingsiteinhumanserumalbumin AT wojciechbal effectofcommonbuffersandheterocyclicligandsonthebindingofcuiiatthemultimetalbindingsiteinhumanserumalbumin |