Elaidic acid leads to mitochondrial dysfunction via mitochondria-associated membranes triggers disruption of mitochondrial calcium fluxes
Elaidic acid (EA) stimulation can lead to endoplasmic reticulum stress (ERS), accompanied by a large release of Ca2+, and ultimately the activation of NLRP3 inflammasome in Kupffer cells (KCs). Mitochondrial instability or dysfunction may be the key stimulating factors to activate NLRP3 inflammasome...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023001349 |
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| _version_ | 1832546432286982144 |
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| author | Hui Liu Xuenan Li Ziyue Wang Lu Li Yucai Li Haiyang Yan Yuan Yuan |
| author_facet | Hui Liu Xuenan Li Ziyue Wang Lu Li Yucai Li Haiyang Yan Yuan Yuan |
| author_sort | Hui Liu |
| collection | DOAJ |
| description | Elaidic acid (EA) stimulation can lead to endoplasmic reticulum stress (ERS), accompanied by a large release of Ca2+, and ultimately the activation of NLRP3 inflammasome in Kupffer cells (KCs). Mitochondrial instability or dysfunction may be the key stimulating factors to activate NLRP3 inflammasome, and sustained Ca2+ transfer can result in mitochondrial dysfunction. We focused on KCs to explore the damage to mitochondria by EA. After EA stimulation, cells produced an oxidative stress (OS) response with a significant increase in ROS release. Immunoprecipitation experiments and the addition of inhibitors revealed that the increase in the level of intracellular Ca2+ led to Ca2+ accumulation in the mitochondrial matrix via mitochondria-associated membranes (MAMs). This was accompanied by a significant release of mROS, loss of MMP and ATP, and a significant increase in mitochondrial permeability transition pore opening, ultimately leading to mitochondrial instability. These findings confirmed the mechanism that EA induced mitochondrial Ca2+ imbalance in KCs via MAM, ultimately leading to mitochondrial dysfunction. Meanwhile, EA induced OS and the decrease of MMP and ATP in rat liver, and significant lesions were found in liver mitochondria. Swelling of the inner mitochondrial cristae and mitochondrial vacuolization occurred, with a marked increase in lipid droplets. |
| format | Article |
| id | doaj-art-dbfcedbaf7114e40a1146d8b841cc1f5 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-dbfcedbaf7114e40a1146d8b841cc1f52025-02-03T06:57:18ZengTsinghua University PressFood Science and Human Wellness2213-45302024-01-01131287298Elaidic acid leads to mitochondrial dysfunction via mitochondria-associated membranes triggers disruption of mitochondrial calcium fluxesHui Liu0Xuenan Li1Ziyue Wang2Lu Li3Yucai Li4Haiyang Yan5Yuan Yuan6College of Food Science and Engineering, Jilin University, Changchun 130062, ChinaCollege of Food Science and Engineering, Jilin University, Changchun 130062, ChinaCollege of Food Science and Engineering, Jilin University, Changchun 130062, ChinaCollege of Food Science and Engineering, Jilin University, Changchun 130062, ChinaCollege of Food Science and Engineering, Jilin University, Changchun 130062, ChinaCollege of Food Science and Engineering, Jilin University, Changchun 130062, ChinaCorresponding author at: College of Food Science and Engineering, Jilin University, Changchun 130062, China.; College of Food Science and Engineering, Jilin University, Changchun 130062, ChinaElaidic acid (EA) stimulation can lead to endoplasmic reticulum stress (ERS), accompanied by a large release of Ca2+, and ultimately the activation of NLRP3 inflammasome in Kupffer cells (KCs). Mitochondrial instability or dysfunction may be the key stimulating factors to activate NLRP3 inflammasome, and sustained Ca2+ transfer can result in mitochondrial dysfunction. We focused on KCs to explore the damage to mitochondria by EA. After EA stimulation, cells produced an oxidative stress (OS) response with a significant increase in ROS release. Immunoprecipitation experiments and the addition of inhibitors revealed that the increase in the level of intracellular Ca2+ led to Ca2+ accumulation in the mitochondrial matrix via mitochondria-associated membranes (MAMs). This was accompanied by a significant release of mROS, loss of MMP and ATP, and a significant increase in mitochondrial permeability transition pore opening, ultimately leading to mitochondrial instability. These findings confirmed the mechanism that EA induced mitochondrial Ca2+ imbalance in KCs via MAM, ultimately leading to mitochondrial dysfunction. Meanwhile, EA induced OS and the decrease of MMP and ATP in rat liver, and significant lesions were found in liver mitochondria. Swelling of the inner mitochondrial cristae and mitochondrial vacuolization occurred, with a marked increase in lipid droplets.http://www.sciencedirect.com/science/article/pii/S2213453023001349Elaidic acid (EA)Mitochondria-associated membranes (MAMs)CalciumEndoplasmic reticulumMitochondria dysfunction |
| spellingShingle | Hui Liu Xuenan Li Ziyue Wang Lu Li Yucai Li Haiyang Yan Yuan Yuan Elaidic acid leads to mitochondrial dysfunction via mitochondria-associated membranes triggers disruption of mitochondrial calcium fluxes Food Science and Human Wellness Elaidic acid (EA) Mitochondria-associated membranes (MAMs) Calcium Endoplasmic reticulum Mitochondria dysfunction |
| title | Elaidic acid leads to mitochondrial dysfunction via mitochondria-associated membranes triggers disruption of mitochondrial calcium fluxes |
| title_full | Elaidic acid leads to mitochondrial dysfunction via mitochondria-associated membranes triggers disruption of mitochondrial calcium fluxes |
| title_fullStr | Elaidic acid leads to mitochondrial dysfunction via mitochondria-associated membranes triggers disruption of mitochondrial calcium fluxes |
| title_full_unstemmed | Elaidic acid leads to mitochondrial dysfunction via mitochondria-associated membranes triggers disruption of mitochondrial calcium fluxes |
| title_short | Elaidic acid leads to mitochondrial dysfunction via mitochondria-associated membranes triggers disruption of mitochondrial calcium fluxes |
| title_sort | elaidic acid leads to mitochondrial dysfunction via mitochondria associated membranes triggers disruption of mitochondrial calcium fluxes |
| topic | Elaidic acid (EA) Mitochondria-associated membranes (MAMs) Calcium Endoplasmic reticulum Mitochondria dysfunction |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023001349 |
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