Silica Nanoparticles Effects on Blood Coagulation Proteins and Platelets
Interaction of nanoparticles with the blood coagulation is important prior to their using as the drug carriers or therapeutic agents. The aim of present work was studying of the primary effects of silica nanoparticles (SiNPs) on haemostasis in vitro. We studied the effect of SiNPs on blood coagulati...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2016/2959414 |
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| author | Volodymyr Gryshchuk Natalya Galagan |
| author_facet | Volodymyr Gryshchuk Natalya Galagan |
| author_sort | Volodymyr Gryshchuk |
| collection | DOAJ |
| description | Interaction of nanoparticles with the blood coagulation is important prior to their using as the drug carriers or therapeutic agents. The aim of present work was studying of the primary effects of silica nanoparticles (SiNPs) on haemostasis in vitro. We studied the effect of SiNPs on blood coagulation directly estimating the activation of prothrombin and factor X and to verify any possible effect of SiNPs on human platelets. It was shown that SiNPs shortened coagulation time in APTT and PT tests and increased the activation of factor X induced by RVV possibly due to the sorption of intrinsic pathway factors on their surface. SiNPs inhibited the aggregation of platelet rich plasma induced by ADP but in the same time partially activated platelets as it was shown using flow cytometry. The possibility of SiNPs usage in nanomedicine is strongly dependant on their final concentration in bloodstream and the size of the particles that are used. However SiNPs are extremely promising as the haemostatic agents for preventing the blood loss after damage. |
| format | Article |
| id | doaj-art-dbf66fd1b069482dadcbb4f50df9565a |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-dbf66fd1b069482dadcbb4f50df9565a2025-02-03T05:57:11ZengWileyBiochemistry Research International2090-22472090-22552016-01-01201610.1155/2016/29594142959414Silica Nanoparticles Effects on Blood Coagulation Proteins and PlateletsVolodymyr Gryshchuk0Natalya Galagan1ESC “Institute of Biology”, Kyiv National Taras Shevchenko University, 64/13 Volodymyrska Street, Kyiv 01601, UkraineChuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, 17 General Naumov Street, Kyiv 03164, UkraineInteraction of nanoparticles with the blood coagulation is important prior to their using as the drug carriers or therapeutic agents. The aim of present work was studying of the primary effects of silica nanoparticles (SiNPs) on haemostasis in vitro. We studied the effect of SiNPs on blood coagulation directly estimating the activation of prothrombin and factor X and to verify any possible effect of SiNPs on human platelets. It was shown that SiNPs shortened coagulation time in APTT and PT tests and increased the activation of factor X induced by RVV possibly due to the sorption of intrinsic pathway factors on their surface. SiNPs inhibited the aggregation of platelet rich plasma induced by ADP but in the same time partially activated platelets as it was shown using flow cytometry. The possibility of SiNPs usage in nanomedicine is strongly dependant on their final concentration in bloodstream and the size of the particles that are used. However SiNPs are extremely promising as the haemostatic agents for preventing the blood loss after damage.http://dx.doi.org/10.1155/2016/2959414 |
| spellingShingle | Volodymyr Gryshchuk Natalya Galagan Silica Nanoparticles Effects on Blood Coagulation Proteins and Platelets Biochemistry Research International |
| title | Silica Nanoparticles Effects on Blood Coagulation Proteins and Platelets |
| title_full | Silica Nanoparticles Effects on Blood Coagulation Proteins and Platelets |
| title_fullStr | Silica Nanoparticles Effects on Blood Coagulation Proteins and Platelets |
| title_full_unstemmed | Silica Nanoparticles Effects on Blood Coagulation Proteins and Platelets |
| title_short | Silica Nanoparticles Effects on Blood Coagulation Proteins and Platelets |
| title_sort | silica nanoparticles effects on blood coagulation proteins and platelets |
| url | http://dx.doi.org/10.1155/2016/2959414 |
| work_keys_str_mv | AT volodymyrgryshchuk silicananoparticleseffectsonbloodcoagulationproteinsandplatelets AT natalyagalagan silicananoparticleseffectsonbloodcoagulationproteinsandplatelets |