Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> Isolated from Raw Cow’s Milk
<i>Escherichia coli (E. coli)</i> is one of the most common pathogens in both humans and livestock. This study aimed to investigate the prevalence of <i>E. coli</i> isolated from raw cow milk and evaluate its antimicrobial resistance rates. A total of 1696 milk samples were c...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/1/209 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587886495531008 |
|---|---|
| author | Roxana Ionela Drugea Mădălina Iulia Siteavu Elena Pitoiu Cristina Delcaru Ecaterina Monica Sârbu Carmen Postolache Stelian Bărăităreanu |
| author_facet | Roxana Ionela Drugea Mădălina Iulia Siteavu Elena Pitoiu Cristina Delcaru Ecaterina Monica Sârbu Carmen Postolache Stelian Bărăităreanu |
| author_sort | Roxana Ionela Drugea |
| collection | DOAJ |
| description | <i>Escherichia coli (E. coli)</i> is one of the most common pathogens in both humans and livestock. This study aimed to investigate the prevalence of <i>E. coli</i> isolated from raw cow milk and evaluate its antimicrobial resistance rates. A total of 1696 milk samples were collected from Romanian dairy farms from 2018 to 2022. <i>E. coli</i> was isolated on various selective agar media, such as Cled agar and Columbia Agar with 5% Sheep Blood. The identification of <i>E. coli</i> was performed by MALDI-TOF MS. <i>E. coli</i> isolates were tested for their susceptibility against 18 commonly used antibiotics in a disk diffusion method. The overall prevalence of <i>E. coli</i> was 22.45% of all isolated pathogens. Antibiogram analysis revealed that 27.51% of <i>E. coli</i> isolates from milk were multidrug-resistant. Resistance was highest for penicillin–novobiocin (87.78%), followed by streptomycin (53.7%). Resistance to six drugs (amoxicillin, streptomycin, kanamycin–cephalexin, marbofloxacin, ampicillin) showed a significant increasing trend over time, while for two drugs (penicillin G-framycetin, doxycycline), a significant decrease was observed. Our results suggest that milk can be a reservoir of bacteria with the potential for infection in humans via the food chain. Furthermore, there is a need for surveillance and monitoring to control the increase in resistance to currently used antimicrobials in dairy farms because the occurrence of multidrug-resistant <i>E. coli</i> isolated from milk poses a health hazard to consumers. |
| format | Article |
| id | doaj-art-dbe7c418f7664e3bb75611218f81a232 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-dbe7c418f7664e3bb75611218f81a2322025-01-24T13:43:04ZengMDPI AGMicroorganisms2076-26072025-01-0113120910.3390/microorganisms13010209Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> Isolated from Raw Cow’s MilkRoxana Ionela Drugea0Mădălina Iulia Siteavu1Elena Pitoiu2Cristina Delcaru3Ecaterina Monica Sârbu4Carmen Postolache5Stelian Bărăităreanu6Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 050097 Bucharest, RomaniaFaculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 050097 Bucharest, RomaniaSynevovet Laboratory, Ilfov County, 077040 Chiajna, RomaniaFaculty of Biology, University of Bucharest, 050663 Bucharest, RomaniaFaculty of Biology, University of Bucharest, 050663 Bucharest, RomaniaFaculty of Biology, University of Bucharest, 050663 Bucharest, RomaniaFaculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 050097 Bucharest, Romania<i>Escherichia coli (E. coli)</i> is one of the most common pathogens in both humans and livestock. This study aimed to investigate the prevalence of <i>E. coli</i> isolated from raw cow milk and evaluate its antimicrobial resistance rates. A total of 1696 milk samples were collected from Romanian dairy farms from 2018 to 2022. <i>E. coli</i> was isolated on various selective agar media, such as Cled agar and Columbia Agar with 5% Sheep Blood. The identification of <i>E. coli</i> was performed by MALDI-TOF MS. <i>E. coli</i> isolates were tested for their susceptibility against 18 commonly used antibiotics in a disk diffusion method. The overall prevalence of <i>E. coli</i> was 22.45% of all isolated pathogens. Antibiogram analysis revealed that 27.51% of <i>E. coli</i> isolates from milk were multidrug-resistant. Resistance was highest for penicillin–novobiocin (87.78%), followed by streptomycin (53.7%). Resistance to six drugs (amoxicillin, streptomycin, kanamycin–cephalexin, marbofloxacin, ampicillin) showed a significant increasing trend over time, while for two drugs (penicillin G-framycetin, doxycycline), a significant decrease was observed. Our results suggest that milk can be a reservoir of bacteria with the potential for infection in humans via the food chain. Furthermore, there is a need for surveillance and monitoring to control the increase in resistance to currently used antimicrobials in dairy farms because the occurrence of multidrug-resistant <i>E. coli</i> isolated from milk poses a health hazard to consumers.https://www.mdpi.com/2076-2607/13/1/209drug resistancedairy farmsbovine mastitisRomaniapublic health |
| spellingShingle | Roxana Ionela Drugea Mădălina Iulia Siteavu Elena Pitoiu Cristina Delcaru Ecaterina Monica Sârbu Carmen Postolache Stelian Bărăităreanu Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> Isolated from Raw Cow’s Milk Microorganisms drug resistance dairy farms bovine mastitis Romania public health |
| title | Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> Isolated from Raw Cow’s Milk |
| title_full | Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> Isolated from Raw Cow’s Milk |
| title_fullStr | Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> Isolated from Raw Cow’s Milk |
| title_full_unstemmed | Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> Isolated from Raw Cow’s Milk |
| title_short | Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> Isolated from Raw Cow’s Milk |
| title_sort | prevalence and antibiotic resistance of i escherichia coli i isolated from raw cow s milk |
| topic | drug resistance dairy farms bovine mastitis Romania public health |
| url | https://www.mdpi.com/2076-2607/13/1/209 |
| work_keys_str_mv | AT roxanaioneladrugea prevalenceandantibioticresistanceofiescherichiacoliiisolatedfromrawcowsmilk AT madalinaiuliasiteavu prevalenceandantibioticresistanceofiescherichiacoliiisolatedfromrawcowsmilk AT elenapitoiu prevalenceandantibioticresistanceofiescherichiacoliiisolatedfromrawcowsmilk AT cristinadelcaru prevalenceandantibioticresistanceofiescherichiacoliiisolatedfromrawcowsmilk AT ecaterinamonicasarbu prevalenceandantibioticresistanceofiescherichiacoliiisolatedfromrawcowsmilk AT carmenpostolache prevalenceandantibioticresistanceofiescherichiacoliiisolatedfromrawcowsmilk AT stelianbaraitareanu prevalenceandantibioticresistanceofiescherichiacoliiisolatedfromrawcowsmilk |