Mouse Reporter Strain for Noninvasive Bioluminescent Imaging of Cells that have Undergone Cre-Mediated Recombination
Conditional alleles containing LoxP recombination sites, in conjunction with Cre recombinase delivered by a variety of means, allows for spatial and temporal control of gene expression in mouse models. Here we describe a mouse strain in which a luciferase (Luc) cDNA, preceded by a LoxP-stop-LoxP (L-...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2003-10-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200303154 |
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| _version_ | 1832544647725973504 |
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| author | Michal Safran William Y. Kim Andrew L. Kung James W. Horner Ron A. DePinho William G. Kaelin |
| author_facet | Michal Safran William Y. Kim Andrew L. Kung James W. Horner Ron A. DePinho William G. Kaelin |
| author_sort | Michal Safran |
| collection | DOAJ |
| description | Conditional alleles containing LoxP recombination sites, in conjunction with Cre recombinase delivered by a variety of means, allows for spatial and temporal control of gene expression in mouse models. Here we describe a mouse strain in which a luciferase (Luc) cDNA, preceded by a LoxP-stop-LoxP (L-S-L) cassette, was introduced into the ubiquitously expressed ROSA26 locus. Mouse embryo fibroblasts derived from this strain expressed luciferase after Cre-mediated recombination in vitro. ROSA26 L-S-L-Luc /+ mice expressed luciferase in a diffuse or liver-restricted pattern, as determined by noninvasive, bioluminescent imaging, when crossed to transgenic mice in which Cre was under the control of a zygotically expressed (EIIA-Cre), or a liver-restricted (albumin-Cre), promoter, respectively. Organ-specific luciferase expression was also seen after intraparenchymal administration of an adenovirus encoding Cre. The ROSA26 L-S-L-Luc /+ strain should be useful for characterizing Cre mouse strains and for following the fate of cells that have undergone Cre-mediated recombination in vivo. |
| format | Article |
| id | doaj-art-dbe6f2f367864f1e9dc51d510c172587 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2003-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-dbe6f2f367864f1e9dc51d510c1725872025-02-03T10:07:59ZengSAGE PublishingMolecular Imaging1536-01212003-10-01210.1162/1535350020030315410.1162_15353500200303154Mouse Reporter Strain for Noninvasive Bioluminescent Imaging of Cells that have Undergone Cre-Mediated RecombinationMichal Safran0William Y. Kim1Andrew L. Kung2James W. Horner3Ron A. DePinho4William G. Kaelin5Dana-Farber Cancer Institute and Brigham and Women's Hospital, Harvard Medical SchoolDana-Farber Cancer Institute and Brigham and Women's Hospital, Harvard Medical SchoolDana-Farber Cancer Institute and Brigham and Women's Hospital, Harvard Medical SchoolDana-Farber Cancer Institute and Brigham and Women's Hospital, Harvard Medical SchoolDana-Farber Cancer Institute and Brigham and Women's Hospital, Harvard Medical SchoolHoward Hughes Medical InstituteConditional alleles containing LoxP recombination sites, in conjunction with Cre recombinase delivered by a variety of means, allows for spatial and temporal control of gene expression in mouse models. Here we describe a mouse strain in which a luciferase (Luc) cDNA, preceded by a LoxP-stop-LoxP (L-S-L) cassette, was introduced into the ubiquitously expressed ROSA26 locus. Mouse embryo fibroblasts derived from this strain expressed luciferase after Cre-mediated recombination in vitro. ROSA26 L-S-L-Luc /+ mice expressed luciferase in a diffuse or liver-restricted pattern, as determined by noninvasive, bioluminescent imaging, when crossed to transgenic mice in which Cre was under the control of a zygotically expressed (EIIA-Cre), or a liver-restricted (albumin-Cre), promoter, respectively. Organ-specific luciferase expression was also seen after intraparenchymal administration of an adenovirus encoding Cre. The ROSA26 L-S-L-Luc /+ strain should be useful for characterizing Cre mouse strains and for following the fate of cells that have undergone Cre-mediated recombination in vivo.https://doi.org/10.1162/15353500200303154 |
| spellingShingle | Michal Safran William Y. Kim Andrew L. Kung James W. Horner Ron A. DePinho William G. Kaelin Mouse Reporter Strain for Noninvasive Bioluminescent Imaging of Cells that have Undergone Cre-Mediated Recombination Molecular Imaging |
| title | Mouse Reporter Strain for Noninvasive Bioluminescent Imaging of Cells that have Undergone Cre-Mediated Recombination |
| title_full | Mouse Reporter Strain for Noninvasive Bioluminescent Imaging of Cells that have Undergone Cre-Mediated Recombination |
| title_fullStr | Mouse Reporter Strain for Noninvasive Bioluminescent Imaging of Cells that have Undergone Cre-Mediated Recombination |
| title_full_unstemmed | Mouse Reporter Strain for Noninvasive Bioluminescent Imaging of Cells that have Undergone Cre-Mediated Recombination |
| title_short | Mouse Reporter Strain for Noninvasive Bioluminescent Imaging of Cells that have Undergone Cre-Mediated Recombination |
| title_sort | mouse reporter strain for noninvasive bioluminescent imaging of cells that have undergone cre mediated recombination |
| url | https://doi.org/10.1162/15353500200303154 |
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