Effects of in-ovo injection of Yu ping feng polysaccharides on growth performance, intestinal development, and immunity in broiler chickens
This study aimed to investigate the effects of the in-ovo injection of Yu Ping Feng polysaccharides (YPF) on the immunological development, hatchability, growth performance, intestinal tissue development, intestinal IgA+ cell distribution, and intestinal secretory IgA (SIgA) production in broiler ch...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011520 |
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| author | Li-Gang Xue Tian-Kui Guo Juan Wang Yu-Quan Shan Li Guo Dong-Xing Zhang Zhong Wei Dan Wang |
| author_facet | Li-Gang Xue Tian-Kui Guo Juan Wang Yu-Quan Shan Li Guo Dong-Xing Zhang Zhong Wei Dan Wang |
| author_sort | Li-Gang Xue |
| collection | DOAJ |
| description | This study aimed to investigate the effects of the in-ovo injection of Yu Ping Feng polysaccharides (YPF) on the immunological development, hatchability, growth performance, intestinal tissue development, intestinal IgA+ cell distribution, and intestinal secretory IgA (SIgA) production in broiler chicken. Herein, 800 chicken embryos were randomly divided into Astragalus polysaccharide (APS), Atractylodes macrocephala polysaccharide (ATR), YPF, and normal saline (control) injection groups—polysaccharide injection, 0.5 mL of 4 mg/mL polysaccharide; normal saline injection, 0.5 mL. The related indexes of hatched chicks were detected, and there were 5 repeats in each group. Compared with the other three groups, the in-ovo YPF injection did not affect the hatching rate of chicken embryos; however, the initial body weight of hatchlings significantly increased and the feed conversion ratio decreased. Additionally, at each time point, the intestinal villus height (VH) of the chicks in the YPF group increased, whereas no notable difference was observed in the crypt depth (CD), resulting in a higher VH/CD ratio. Furthermore, the YPF group exhibited a statistically significant increase in intestinal IgA+ cell count and flushing fluid SIgA level throughout various time periods compared with those in the other three groups. Additionally, the expression of intestinal mucosal immune cytokines, including interleukin (IL)-2, IL-4, IL-6, and interferon-γ, were markedly increased in the duodenum and ileum of the YPF group. Moreover, the analysis of immune development revealed that their serum levels in the polysaccharide-injected groups were also increased, with the YPF groups exhibiting superior performance than the APS and ATR groups and encouraging the development of T and B lymphocytes in the spleen and peripheral blood mononuclear cells. Altogether, the findings of this study demonstrate that the in-ovo injection of YPF can improve the growth performance, intestinal tissue development, and immune system of the broiler chicks. |
| format | Article |
| id | doaj-art-dbc14039186b41c6a1dfb30020322cd2 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-dbc14039186b41c6a1dfb30020322cd22025-01-22T05:40:32ZengElsevierPoultry Science0032-57912025-01-011041104574Effects of in-ovo injection of Yu ping feng polysaccharides on growth performance, intestinal development, and immunity in broiler chickensLi-Gang Xue0Tian-Kui Guo1Juan Wang2Yu-Quan Shan3Li Guo4Dong-Xing Zhang5Zhong Wei6Dan Wang7Jilin Agricultural Science and Technology University, 1 Xuefu Road Zuojia Town, Changyi District, Jilin, 132109, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaJilin Agricultural Science and Technology University, 1 Xuefu Road Zuojia Town, Changyi District, Jilin, 132109, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaJilin Agricultural Science and Technology University, 1 Xuefu Road Zuojia Town, Changyi District, Jilin, 132109, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaJilin Agricultural Science and Technology University, 1 Xuefu Road Zuojia Town, Changyi District, Jilin, 132109, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaJilin Agricultural Science and Technology University, 1 Xuefu Road Zuojia Town, Changyi District, Jilin, 132109, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaJilin Agricultural Science and Technology University, 1 Xuefu Road Zuojia Town, Changyi District, Jilin, 132109, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaJilin Agricultural Science and Technology University, 1 Xuefu Road Zuojia Town, Changyi District, Jilin, 132109, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaCorresponding author.; Jilin Agricultural Science and Technology University, 1 Xuefu Road Zuojia Town, Changyi District, Jilin, 132109, China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, ChinaThis study aimed to investigate the effects of the in-ovo injection of Yu Ping Feng polysaccharides (YPF) on the immunological development, hatchability, growth performance, intestinal tissue development, intestinal IgA+ cell distribution, and intestinal secretory IgA (SIgA) production in broiler chicken. Herein, 800 chicken embryos were randomly divided into Astragalus polysaccharide (APS), Atractylodes macrocephala polysaccharide (ATR), YPF, and normal saline (control) injection groups—polysaccharide injection, 0.5 mL of 4 mg/mL polysaccharide; normal saline injection, 0.5 mL. The related indexes of hatched chicks were detected, and there were 5 repeats in each group. Compared with the other three groups, the in-ovo YPF injection did not affect the hatching rate of chicken embryos; however, the initial body weight of hatchlings significantly increased and the feed conversion ratio decreased. Additionally, at each time point, the intestinal villus height (VH) of the chicks in the YPF group increased, whereas no notable difference was observed in the crypt depth (CD), resulting in a higher VH/CD ratio. Furthermore, the YPF group exhibited a statistically significant increase in intestinal IgA+ cell count and flushing fluid SIgA level throughout various time periods compared with those in the other three groups. Additionally, the expression of intestinal mucosal immune cytokines, including interleukin (IL)-2, IL-4, IL-6, and interferon-γ, were markedly increased in the duodenum and ileum of the YPF group. Moreover, the analysis of immune development revealed that their serum levels in the polysaccharide-injected groups were also increased, with the YPF groups exhibiting superior performance than the APS and ATR groups and encouraging the development of T and B lymphocytes in the spleen and peripheral blood mononuclear cells. Altogether, the findings of this study demonstrate that the in-ovo injection of YPF can improve the growth performance, intestinal tissue development, and immune system of the broiler chicks.http://www.sciencedirect.com/science/article/pii/S0032579124011520Yupingfeng polysaccharideIn ovo injectionIntestinal developmentImmune developmentBroiler |
| spellingShingle | Li-Gang Xue Tian-Kui Guo Juan Wang Yu-Quan Shan Li Guo Dong-Xing Zhang Zhong Wei Dan Wang Effects of in-ovo injection of Yu ping feng polysaccharides on growth performance, intestinal development, and immunity in broiler chickens Poultry Science Yupingfeng polysaccharide In ovo injection Intestinal development Immune development Broiler |
| title | Effects of in-ovo injection of Yu ping feng polysaccharides on growth performance, intestinal development, and immunity in broiler chickens |
| title_full | Effects of in-ovo injection of Yu ping feng polysaccharides on growth performance, intestinal development, and immunity in broiler chickens |
| title_fullStr | Effects of in-ovo injection of Yu ping feng polysaccharides on growth performance, intestinal development, and immunity in broiler chickens |
| title_full_unstemmed | Effects of in-ovo injection of Yu ping feng polysaccharides on growth performance, intestinal development, and immunity in broiler chickens |
| title_short | Effects of in-ovo injection of Yu ping feng polysaccharides on growth performance, intestinal development, and immunity in broiler chickens |
| title_sort | effects of in ovo injection of yu ping feng polysaccharides on growth performance intestinal development and immunity in broiler chickens |
| topic | Yupingfeng polysaccharide In ovo injection Intestinal development Immune development Broiler |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011520 |
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