De Novo Donor-Specific HLA Antibodies Developing Early or Late after Transplant Are Associated with the Same Risk of Graft Damage and Loss in Nonsensitized Kidney Recipients

De novo posttransplant donor-specific HLA-antibody (dnDSA) detection is now recognized as a tool to identify patients at risk for antibody-mediated rejection (AMR) and graft loss. It is still unclear whether the time interval from transplant to DSA occurrence influences graft damage. Utilizing sera...

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Main Authors: Michela Cioni, Arcangelo Nocera, Annalisa Innocente, Augusto Tagliamacco, Antonella Trivelli, Sabrina Basso, Giuseppe Quartuccio, Iris Fontana, Alberto Magnasco, Francesca Drago, Antonella Gurrado, Ilaria Guido, Francesca Compagno, Giacomo Garibotto, Catherine Klersy, Enrico Verrina, Gian Marco Ghiggeri, Massimo Cardillo, Patrizia Comoli, Fabrizio Ginevri
Format: Article
Language:English
Published: Wiley 2017-01-01
Series:Journal of Immunology Research
Online Access:http://dx.doi.org/10.1155/2017/1747030
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author Michela Cioni
Arcangelo Nocera
Annalisa Innocente
Augusto Tagliamacco
Antonella Trivelli
Sabrina Basso
Giuseppe Quartuccio
Iris Fontana
Alberto Magnasco
Francesca Drago
Antonella Gurrado
Ilaria Guido
Francesca Compagno
Giacomo Garibotto
Catherine Klersy
Enrico Verrina
Gian Marco Ghiggeri
Massimo Cardillo
Patrizia Comoli
Fabrizio Ginevri
author_facet Michela Cioni
Arcangelo Nocera
Annalisa Innocente
Augusto Tagliamacco
Antonella Trivelli
Sabrina Basso
Giuseppe Quartuccio
Iris Fontana
Alberto Magnasco
Francesca Drago
Antonella Gurrado
Ilaria Guido
Francesca Compagno
Giacomo Garibotto
Catherine Klersy
Enrico Verrina
Gian Marco Ghiggeri
Massimo Cardillo
Patrizia Comoli
Fabrizio Ginevri
author_sort Michela Cioni
collection DOAJ
description De novo posttransplant donor-specific HLA-antibody (dnDSA) detection is now recognized as a tool to identify patients at risk for antibody-mediated rejection (AMR) and graft loss. It is still unclear whether the time interval from transplant to DSA occurrence influences graft damage. Utilizing sera collected longitudinally, we evaluated 114 consecutive primary pediatric kidney recipients grafted between 2002 and 2013 for dnDSA occurrence by Luminex platform. dnDSAs occurred in 39 patients at a median time of 24.6 months. In 15 patients, dnDSAs developed within 1 year (early-onset group), while the other 24 seroconverted after the first posttransplant year (late-onset group). The two groups were comparable when considering patient- and transplant-related factors, as well as DSA biological properties, including C1q and C3d complement-binding ability. Only recipient age at transplant significantly differed in the two cohorts, with younger patients showing earlier dnDSA development. Late AMR was diagnosed in 47% of the early group and in 58% of the late group. Graft loss occurred in 3/15 (20%) and 4/24 (17%) patients in early- and late-onset groups, respectively (p = ns). In our pediatric kidney recipients, dnDSAs predict AMR and graft loss irrespective of the time elapsed between transplantation and antibody occurrence.
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spelling doaj-art-dbb50f9e59ab4d9b922d9a99d2e7f7392025-08-20T02:21:30ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/17470301747030De Novo Donor-Specific HLA Antibodies Developing Early or Late after Transplant Are Associated with the Same Risk of Graft Damage and Loss in Nonsensitized Kidney RecipientsMichela Cioni0Arcangelo Nocera1Annalisa Innocente2Augusto Tagliamacco3Antonella Trivelli4Sabrina Basso5Giuseppe Quartuccio6Iris Fontana7Alberto Magnasco8Francesca Drago9Antonella Gurrado10Ilaria Guido11Francesca Compagno12Giacomo Garibotto13Catherine Klersy14Enrico Verrina15Gian Marco Ghiggeri16Massimo Cardillo17Patrizia Comoli18Fabrizio Ginevri19Nephrology, Dialysis and Transplantation Unit, IRCCS Istituto G. Gaslini, Genova, ItalyClinical Nephrology Unit and Transplant Immunology Research Laboratory, Department of Internal Medicine, University of Genova and IRCCS San Martino University Hospital IST, Genova, ItalyTransplantation Immunology, IRCCS Fondazione Ca’ Granda, Ospedale Maggiore Policlinico, Milano, ItalyClinical Nephrology Unit and Transplant Immunology Research Laboratory, Department of Internal Medicine, University of Genova and IRCCS San Martino University Hospital IST, Genova, ItalyNephrology, Dialysis and Transplantation Unit, IRCCS Istituto G. Gaslini, Genova, ItalyPediatric Hematology/Oncology and Cell Factory, Fondazione IRCCS Policlinico S. Matteo, Pavia, ItalyPediatric Hematology/Oncology and Cell Factory, Fondazione IRCCS Policlinico S. Matteo, Pavia, ItalyVascular and Endovascular Unit and Kidney Transplant Surgery Unit, University of Genova, IRCCS San Martino University Hospital IST, Genova, ItalyNephrology, Dialysis and Transplantation Unit, IRCCS Istituto G. Gaslini, Genova, ItalyTransplantation Immunology, IRCCS Fondazione Ca’ Granda, Ospedale Maggiore Policlinico, Milano, ItalyPediatric Hematology/Oncology and Cell Factory, Fondazione IRCCS Policlinico S. Matteo, Pavia, ItalyPediatric Hematology/Oncology and Cell Factory, Fondazione IRCCS Policlinico S. Matteo, Pavia, ItalyPediatric Hematology/Oncology and Cell Factory, Fondazione IRCCS Policlinico S. Matteo, Pavia, ItalyClinical Nephrology Unit and Transplant Immunology Research Laboratory, Department of Internal Medicine, University of Genova and IRCCS San Martino University Hospital IST, Genova, ItalyBiometry and Statistics Service, Fondazione IRCCS Policlinico S. Matteo, Pavia, ItalyNephrology, Dialysis and Transplantation Unit, IRCCS Istituto G. Gaslini, Genova, ItalyNephrology, Dialysis and Transplantation Unit, IRCCS Istituto G. Gaslini, Genova, ItalyTransplantation Immunology, IRCCS Fondazione Ca’ Granda, Ospedale Maggiore Policlinico, Milano, ItalyPediatric Hematology/Oncology and Cell Factory, Fondazione IRCCS Policlinico S. Matteo, Pavia, ItalyNephrology, Dialysis and Transplantation Unit, IRCCS Istituto G. Gaslini, Genova, ItalyDe novo posttransplant donor-specific HLA-antibody (dnDSA) detection is now recognized as a tool to identify patients at risk for antibody-mediated rejection (AMR) and graft loss. It is still unclear whether the time interval from transplant to DSA occurrence influences graft damage. Utilizing sera collected longitudinally, we evaluated 114 consecutive primary pediatric kidney recipients grafted between 2002 and 2013 for dnDSA occurrence by Luminex platform. dnDSAs occurred in 39 patients at a median time of 24.6 months. In 15 patients, dnDSAs developed within 1 year (early-onset group), while the other 24 seroconverted after the first posttransplant year (late-onset group). The two groups were comparable when considering patient- and transplant-related factors, as well as DSA biological properties, including C1q and C3d complement-binding ability. Only recipient age at transplant significantly differed in the two cohorts, with younger patients showing earlier dnDSA development. Late AMR was diagnosed in 47% of the early group and in 58% of the late group. Graft loss occurred in 3/15 (20%) and 4/24 (17%) patients in early- and late-onset groups, respectively (p = ns). In our pediatric kidney recipients, dnDSAs predict AMR and graft loss irrespective of the time elapsed between transplantation and antibody occurrence.http://dx.doi.org/10.1155/2017/1747030
spellingShingle Michela Cioni
Arcangelo Nocera
Annalisa Innocente
Augusto Tagliamacco
Antonella Trivelli
Sabrina Basso
Giuseppe Quartuccio
Iris Fontana
Alberto Magnasco
Francesca Drago
Antonella Gurrado
Ilaria Guido
Francesca Compagno
Giacomo Garibotto
Catherine Klersy
Enrico Verrina
Gian Marco Ghiggeri
Massimo Cardillo
Patrizia Comoli
Fabrizio Ginevri
De Novo Donor-Specific HLA Antibodies Developing Early or Late after Transplant Are Associated with the Same Risk of Graft Damage and Loss in Nonsensitized Kidney Recipients
Journal of Immunology Research
title De Novo Donor-Specific HLA Antibodies Developing Early or Late after Transplant Are Associated with the Same Risk of Graft Damage and Loss in Nonsensitized Kidney Recipients
title_full De Novo Donor-Specific HLA Antibodies Developing Early or Late after Transplant Are Associated with the Same Risk of Graft Damage and Loss in Nonsensitized Kidney Recipients
title_fullStr De Novo Donor-Specific HLA Antibodies Developing Early or Late after Transplant Are Associated with the Same Risk of Graft Damage and Loss in Nonsensitized Kidney Recipients
title_full_unstemmed De Novo Donor-Specific HLA Antibodies Developing Early or Late after Transplant Are Associated with the Same Risk of Graft Damage and Loss in Nonsensitized Kidney Recipients
title_short De Novo Donor-Specific HLA Antibodies Developing Early or Late after Transplant Are Associated with the Same Risk of Graft Damage and Loss in Nonsensitized Kidney Recipients
title_sort de novo donor specific hla antibodies developing early or late after transplant are associated with the same risk of graft damage and loss in nonsensitized kidney recipients
url http://dx.doi.org/10.1155/2017/1747030
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