Glycosylation Patterns in <i>Meccus (Triatoma) pallidipennis</i> Gut: Implications for the Development of Vector Control Strategies
The primary mode of transmission for Chagas disease is vector-borne transmission, spread by hematophagous insects of the <i>Triatominae</i> subfamily. In Mexico, the triatomine <i>Meccus pallidipennis</i> is particularly significant in the transmission of <i>Trypanosoma...
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MDPI AG
2025-01-01
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| author | Elia Torres-Gutiérrez Frida Noelly Candelas-Otero Olivia Alicia Reynoso-Ducoing Berenice González-Rete Mauro Omar Vences-Blanco Margarita Cabrera-Bravo Martha Irene Bucio-Torres Paz María Silvia Salazar-Schettino |
| author_facet | Elia Torres-Gutiérrez Frida Noelly Candelas-Otero Olivia Alicia Reynoso-Ducoing Berenice González-Rete Mauro Omar Vences-Blanco Margarita Cabrera-Bravo Martha Irene Bucio-Torres Paz María Silvia Salazar-Schettino |
| author_sort | Elia Torres-Gutiérrez |
| collection | DOAJ |
| description | The primary mode of transmission for Chagas disease is vector-borne transmission, spread by hematophagous insects of the <i>Triatominae</i> subfamily. In Mexico, the triatomine <i>Meccus pallidipennis</i> is particularly significant in the transmission of <i>Trypanosoma cruzi</i>. This study focused on analyzing protein expression and modifications by glycosylation in different regions of the digestive tract of fifth-instar nymphs of <i>M. pallidipennis</i>. Two gut sections were dissected and extracted: the anterior midgut (AMG) and the proctodeum or rectum (RE). Proteins were extracted from each tissue sample and profiled by one- and two-dimensional electrophoresis; protein glycosylation was analyzed by lectin affinity. Our results showed significant differences in protein expression and glycosylation between both gut regions, with modifications being more frequent in the RE. The proteins HSP70, actin, and tubulin were analyzed, finding a differential expression of the latter two between AMG and RE. Understanding glycosylation patterns provides critical insights into vector–pathogen interactions that could eventually inform novel control approaches. Furthermore, the potential use of lectins as insecticidal agents highlights the broader implications of glycoprotein research in the future development of strategies on vector control to disrupt <i>T. cruzi</i> transmission. |
| format | Article |
| id | doaj-art-dbaf6b6936c34cbab0532b233bafdd2d |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-dbaf6b6936c34cbab0532b233bafdd2d2025-01-24T13:42:28ZengMDPI AGMicroorganisms2076-26072025-01-011315810.3390/microorganisms13010058Glycosylation Patterns in <i>Meccus (Triatoma) pallidipennis</i> Gut: Implications for the Development of Vector Control StrategiesElia Torres-Gutiérrez0Frida Noelly Candelas-Otero1Olivia Alicia Reynoso-Ducoing2Berenice González-Rete3Mauro Omar Vences-Blanco4Margarita Cabrera-Bravo5Martha Irene Bucio-Torres6Paz María Silvia Salazar-Schettino7Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México City 04510, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México City 04510, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México City 04510, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México City 04510, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México City 04510, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México City 04510, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México City 04510, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México City 04510, MexicoThe primary mode of transmission for Chagas disease is vector-borne transmission, spread by hematophagous insects of the <i>Triatominae</i> subfamily. In Mexico, the triatomine <i>Meccus pallidipennis</i> is particularly significant in the transmission of <i>Trypanosoma cruzi</i>. This study focused on analyzing protein expression and modifications by glycosylation in different regions of the digestive tract of fifth-instar nymphs of <i>M. pallidipennis</i>. Two gut sections were dissected and extracted: the anterior midgut (AMG) and the proctodeum or rectum (RE). Proteins were extracted from each tissue sample and profiled by one- and two-dimensional electrophoresis; protein glycosylation was analyzed by lectin affinity. Our results showed significant differences in protein expression and glycosylation between both gut regions, with modifications being more frequent in the RE. The proteins HSP70, actin, and tubulin were analyzed, finding a differential expression of the latter two between AMG and RE. Understanding glycosylation patterns provides critical insights into vector–pathogen interactions that could eventually inform novel control approaches. Furthermore, the potential use of lectins as insecticidal agents highlights the broader implications of glycoprotein research in the future development of strategies on vector control to disrupt <i>T. cruzi</i> transmission.https://www.mdpi.com/2076-2607/13/1/58triatomine gutglycosylation patternsvector controlChagas disease vector |
| spellingShingle | Elia Torres-Gutiérrez Frida Noelly Candelas-Otero Olivia Alicia Reynoso-Ducoing Berenice González-Rete Mauro Omar Vences-Blanco Margarita Cabrera-Bravo Martha Irene Bucio-Torres Paz María Silvia Salazar-Schettino Glycosylation Patterns in <i>Meccus (Triatoma) pallidipennis</i> Gut: Implications for the Development of Vector Control Strategies Microorganisms triatomine gut glycosylation patterns vector control Chagas disease vector |
| title | Glycosylation Patterns in <i>Meccus (Triatoma) pallidipennis</i> Gut: Implications for the Development of Vector Control Strategies |
| title_full | Glycosylation Patterns in <i>Meccus (Triatoma) pallidipennis</i> Gut: Implications for the Development of Vector Control Strategies |
| title_fullStr | Glycosylation Patterns in <i>Meccus (Triatoma) pallidipennis</i> Gut: Implications for the Development of Vector Control Strategies |
| title_full_unstemmed | Glycosylation Patterns in <i>Meccus (Triatoma) pallidipennis</i> Gut: Implications for the Development of Vector Control Strategies |
| title_short | Glycosylation Patterns in <i>Meccus (Triatoma) pallidipennis</i> Gut: Implications for the Development of Vector Control Strategies |
| title_sort | glycosylation patterns in i meccus triatoma pallidipennis i gut implications for the development of vector control strategies |
| topic | triatomine gut glycosylation patterns vector control Chagas disease vector |
| url | https://www.mdpi.com/2076-2607/13/1/58 |
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