Development and Validation of a HPLC Method for Determination of Anastrozole in Tablet Dosage Form
A simple, economic, accurate reverse phase isocratic HPLC method was developed for the quantitation of anastrozole in tablet dosage form. The quantification was carried out using Gracesmart RP18, 5 μ (100 mmx4.6 mm) with UV detected at 215 nm. The elution was achieved isocratically with a mobile pha...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/909064 |
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| author | D. Sathis Kumar A. Harani D. Sridhar David Banji Knv Rao Guruviah Yogesw Aran |
| author_facet | D. Sathis Kumar A. Harani D. Sridhar David Banji Knv Rao Guruviah Yogesw Aran |
| author_sort | D. Sathis Kumar |
| collection | DOAJ |
| description | A simple, economic, accurate reverse phase isocratic HPLC method was developed for the quantitation of anastrozole in tablet dosage form. The quantification was carried out using Gracesmart RP18, 5 μ (100 mmx4.6 mm) with UV detected at 215 nm. The elution was achieved isocratically with a mobile phase comprising a mixture of buffer (pH 6.0) and acetonitrile (1:1, v/v). The flow rate was 1.0 mL/min The procedure was validated as per ICH rules for Accuracy, Precision, Detection limit, Linearity, Reproducibility and Quantitation limit. The linearity concentration range was 10-20 mcg/mL with the correlation coefficient of 0.9935. The percentage recovery for Anastrozole was found to be 97.31±2.2%. Limit of detection and limit of quantitation values were found to be 0.351 mcg/mL and 1.053 mcg/mL. The method has been successfully used to analyze commercial solid dosage containing 1mg of anastrozole with good recoveries and proved to be robust. |
| format | Article |
| id | doaj-art-dba815f11d2547aeb3e7b543e4278a5d |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-dba815f11d2547aeb3e7b543e4278a5d2025-02-03T06:07:59ZengWileyE-Journal of Chemistry0973-49452090-98102011-01-018279479710.1155/2011/909064Development and Validation of a HPLC Method for Determination of Anastrozole in Tablet Dosage FormD. Sathis Kumar0A. Harani1D. Sridhar2David Banji3Knv Rao4Guruviah5Yogesw Aran6Department of Pharmaceutical Analysis, Nalanda College of Pharmacy, Nalgonda-508001, IndiaDepartment of Pharmaceutical Analysis, Nalanda College of Pharmacy, Nalgonda-508001, IndiaDepartment of Pharmaceutical Analysis, Nalanda College of Pharmacy, Nalgonda-508001, IndiaDepartment of Pharmaceutical Analysis, Nalanda College of Pharmacy, Nalgonda-508001, IndiaDepartment of Pharmaceutical Analysis, Nalanda College of Pharmacy, Nalgonda-508001, IndiaMatrix Lab, Hyderabad, IndiaDepartment of Pharmaceutical Analysis, Nalanda College of Pharmacy, Nalgonda-508001, IndiaA simple, economic, accurate reverse phase isocratic HPLC method was developed for the quantitation of anastrozole in tablet dosage form. The quantification was carried out using Gracesmart RP18, 5 μ (100 mmx4.6 mm) with UV detected at 215 nm. The elution was achieved isocratically with a mobile phase comprising a mixture of buffer (pH 6.0) and acetonitrile (1:1, v/v). The flow rate was 1.0 mL/min The procedure was validated as per ICH rules for Accuracy, Precision, Detection limit, Linearity, Reproducibility and Quantitation limit. The linearity concentration range was 10-20 mcg/mL with the correlation coefficient of 0.9935. The percentage recovery for Anastrozole was found to be 97.31±2.2%. Limit of detection and limit of quantitation values were found to be 0.351 mcg/mL and 1.053 mcg/mL. The method has been successfully used to analyze commercial solid dosage containing 1mg of anastrozole with good recoveries and proved to be robust.http://dx.doi.org/10.1155/2011/909064 |
| spellingShingle | D. Sathis Kumar A. Harani D. Sridhar David Banji Knv Rao Guruviah Yogesw Aran Development and Validation of a HPLC Method for Determination of Anastrozole in Tablet Dosage Form E-Journal of Chemistry |
| title | Development and Validation of a HPLC Method for Determination of Anastrozole in Tablet Dosage Form |
| title_full | Development and Validation of a HPLC Method for Determination of Anastrozole in Tablet Dosage Form |
| title_fullStr | Development and Validation of a HPLC Method for Determination of Anastrozole in Tablet Dosage Form |
| title_full_unstemmed | Development and Validation of a HPLC Method for Determination of Anastrozole in Tablet Dosage Form |
| title_short | Development and Validation of a HPLC Method for Determination of Anastrozole in Tablet Dosage Form |
| title_sort | development and validation of a hplc method for determination of anastrozole in tablet dosage form |
| url | http://dx.doi.org/10.1155/2011/909064 |
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