Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels
A method has been developed to induce and retain a contractile phenotype for vascular smooth muscle cells, as the first step towards the development of a biomimetic blood vessel construct with minimal compliance mismatch. Melt spun PCL fibers were deposited on a mandrel to form aligned fibers of 10 ...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2015/434876 |
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| author | Animesh Agrawal Bae Hoon Lee Scott A. Irvine Jia An Ramya Bhuthalingam Vaishali Singh Kok Yao Low Chee Kai Chua Subbu S. Venkatraman |
| author_facet | Animesh Agrawal Bae Hoon Lee Scott A. Irvine Jia An Ramya Bhuthalingam Vaishali Singh Kok Yao Low Chee Kai Chua Subbu S. Venkatraman |
| author_sort | Animesh Agrawal |
| collection | DOAJ |
| description | A method has been developed to induce and retain a contractile phenotype for vascular smooth muscle cells, as the first step towards the development of a biomimetic blood vessel construct with minimal compliance mismatch. Melt spun PCL fibers were deposited on a mandrel to form aligned fibers of 10 μm in diameter. The fibers were bonded into aligned arrangement through dip coating in chitosan solution. This formed a surface of parallel grooves, 10 μm deep by 10 μm across, presenting a surface layer of chitosan to promote cell surface interactions. The aligned fiber surface was used to culture cells present in the vascular wall, in particular fibroblasts and smooth muscle cells. This topography induced “surface guidance” over the orientation of the cells, which adopted an elongated spindle-like morphology, whereas cells on the unpatterned control surface did not show such orientation, assuming more rhomboid shapes. The preservation of VSMC contractile phenotype on the aligned scaffold was demonstrated by the retention of α-SMA expression after several days of culture. The effect was assessed on a prototype vascular graft prosthesis fabricated from polylactide caprolactone; VSMCs aligned longitudinally along a fiberless tube, whereas, for the aligned fiber coated tubes, the VSMCs aligned in the required circumferential orientation. |
| format | Article |
| id | doaj-art-dba054ef7dde4fc19758a3cad82a5f39 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-dba054ef7dde4fc19758a3cad82a5f392025-02-03T01:30:29ZengWileyInternational Journal of Biomaterials1687-87871687-87952015-01-01201510.1155/2015/434876434876Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood VesselsAnimesh Agrawal0Bae Hoon Lee1Scott A. Irvine2Jia An3Ramya Bhuthalingam4Vaishali Singh5Kok Yao Low6Chee Kai Chua7Subbu S. Venkatraman8Materials and Science Engineering, Nanyang Technological University, N4.1-01-30, 50 Nanyang Avenue, 639798, SingaporeMaterials and Science Engineering, Nanyang Technological University, N4.1-01-30, 50 Nanyang Avenue, 639798, SingaporeMaterials and Science Engineering, Nanyang Technological University, N4.1-01-30, 50 Nanyang Avenue, 639798, SingaporeSchool of Mechanical & Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, SingaporeMaterials and Science Engineering, Nanyang Technological University, N4.1-01-30, 50 Nanyang Avenue, 639798, SingaporeDepartment of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, IndiaSchool of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551, SingaporeSchool of Mechanical & Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, SingaporeMaterials and Science Engineering, Nanyang Technological University, N4.1-01-30, 50 Nanyang Avenue, 639798, SingaporeA method has been developed to induce and retain a contractile phenotype for vascular smooth muscle cells, as the first step towards the development of a biomimetic blood vessel construct with minimal compliance mismatch. Melt spun PCL fibers were deposited on a mandrel to form aligned fibers of 10 μm in diameter. The fibers were bonded into aligned arrangement through dip coating in chitosan solution. This formed a surface of parallel grooves, 10 μm deep by 10 μm across, presenting a surface layer of chitosan to promote cell surface interactions. The aligned fiber surface was used to culture cells present in the vascular wall, in particular fibroblasts and smooth muscle cells. This topography induced “surface guidance” over the orientation of the cells, which adopted an elongated spindle-like morphology, whereas cells on the unpatterned control surface did not show such orientation, assuming more rhomboid shapes. The preservation of VSMC contractile phenotype on the aligned scaffold was demonstrated by the retention of α-SMA expression after several days of culture. The effect was assessed on a prototype vascular graft prosthesis fabricated from polylactide caprolactone; VSMCs aligned longitudinally along a fiberless tube, whereas, for the aligned fiber coated tubes, the VSMCs aligned in the required circumferential orientation.http://dx.doi.org/10.1155/2015/434876 |
| spellingShingle | Animesh Agrawal Bae Hoon Lee Scott A. Irvine Jia An Ramya Bhuthalingam Vaishali Singh Kok Yao Low Chee Kai Chua Subbu S. Venkatraman Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels International Journal of Biomaterials |
| title | Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels |
| title_full | Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels |
| title_fullStr | Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels |
| title_full_unstemmed | Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels |
| title_short | Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels |
| title_sort | smooth muscle cell alignment and phenotype control by melt spun polycaprolactone fibers for seeding of tissue engineered blood vessels |
| url | http://dx.doi.org/10.1155/2015/434876 |
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