T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity
Background and Aims. Nonceliac gluten sensitivity (NCGS) is a gluten-related emerging condition. Since few data about NCGS histopathology is available, we assessed the markers of lymphocyte and innate immunity activation. Materials and Methods. We retrieved duodenal biopsy samples of patients with N...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2017/5023680 |
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| _version_ | 1832562292924874752 |
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| author | Giuseppe Losurdo Domenico Piscitelli Federica Pezzuto Francesco Fortarezza Claudia Covelli Antonella Marra Andrea Iannone Annacinzia Amoruso Mariabeatrice Principi Enzo Ierardi Alfredo Di Leo |
| author_facet | Giuseppe Losurdo Domenico Piscitelli Federica Pezzuto Francesco Fortarezza Claudia Covelli Antonella Marra Andrea Iannone Annacinzia Amoruso Mariabeatrice Principi Enzo Ierardi Alfredo Di Leo |
| author_sort | Giuseppe Losurdo |
| collection | DOAJ |
| description | Background and Aims. Nonceliac gluten sensitivity (NCGS) is a gluten-related emerging condition. Since few data about NCGS histopathology is available, we assessed the markers of lymphocyte and innate immunity activation. Materials and Methods. We retrieved duodenal biopsy samples of patients with NCGS diagnosis according to the Salerno criteria. We selected specimens of positive (seropositive celiac disease/Marsh 1-2 stage) and negative (normal microscopic picture) controls. Immunohistochemistry for CD3 (intraepithelial lymphocytes-IELs), CD4 (T helper lymphocytes), CD8 (T cytotoxic lymphocytes), and CD1a/CD117 (Langerhans/mast cells) was performed. ANOVA plus Bonferroni’s tests were used for statistical analysis. Results. Twenty NCGS, 16 celiac disease, and 16 negative controls were selected. CD3 in NCGS were higher than negative controls and lower than celiac disease (18.5 ± 6.4, 11.9 ± 2.8, and 40.8 ± 8.1 IELs/100 enterocytes; p<0.001). CD4 were lower in NCGS than controls and celiac disease (31.0 ± 22.1, 72.5 ± 29.5, and 103.7 ± 15.7 cells/mm2; p<0.001). CD8 in NCGS were similar to negative controls, but lower than celiac disease (14.0 ± 7.4 and 34.0 ± 7.1 IELs/100 enterocytes, p<0.001). CD117 were higher in NCGS than celiac disease and negative controls (145.8 ± 49.9, 121.3 ± 13.1, and 113.5 ± 23.4 cells/mm2; p=0.009). Conclusions. The combination of CD4 and CD117, as well as IEL characterization, may be useful to support a clinical diagnosis of NCGS. |
| format | Article |
| id | doaj-art-db9807ce823c42809420c5992c7cfcab |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-db9807ce823c42809420c5992c7cfcab2025-02-03T01:23:03ZengWileyGastroenterology Research and Practice1687-61211687-630X2017-01-01201710.1155/2017/50236805023680T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten SensitivityGiuseppe Losurdo0Domenico Piscitelli1Federica Pezzuto2Francesco Fortarezza3Claudia Covelli4Antonella Marra5Andrea Iannone6Annacinzia Amoruso7Mariabeatrice Principi8Enzo Ierardi9Alfredo Di Leo10Section of Gastroenterology, Department of Emergency and Organ Transplantation, University “Aldo Moro”, Bari, ItalySection of Pathology, Department of Emergency and Organ Transplantation, University “Aldo Moro”, Bari, ItalySection of Pathology, Department of Emergency and Organ Transplantation, University “Aldo Moro”, Bari, ItalySection of Pathology, Department of Emergency and Organ Transplantation, University “Aldo Moro”, Bari, ItalySection of Pathology, Department of Emergency and Organ Transplantation, University “Aldo Moro”, Bari, ItalySection of Gastroenterology, Department of Emergency and Organ Transplantation, University “Aldo Moro”, Bari, ItalySection of Gastroenterology, Department of Emergency and Organ Transplantation, University “Aldo Moro”, Bari, ItalySection of Gastroenterology, Department of Emergency and Organ Transplantation, University “Aldo Moro”, Bari, ItalySection of Gastroenterology, Department of Emergency and Organ Transplantation, University “Aldo Moro”, Bari, ItalySection of Gastroenterology, Department of Emergency and Organ Transplantation, University “Aldo Moro”, Bari, ItalySection of Gastroenterology, Department of Emergency and Organ Transplantation, University “Aldo Moro”, Bari, ItalyBackground and Aims. Nonceliac gluten sensitivity (NCGS) is a gluten-related emerging condition. Since few data about NCGS histopathology is available, we assessed the markers of lymphocyte and innate immunity activation. Materials and Methods. We retrieved duodenal biopsy samples of patients with NCGS diagnosis according to the Salerno criteria. We selected specimens of positive (seropositive celiac disease/Marsh 1-2 stage) and negative (normal microscopic picture) controls. Immunohistochemistry for CD3 (intraepithelial lymphocytes-IELs), CD4 (T helper lymphocytes), CD8 (T cytotoxic lymphocytes), and CD1a/CD117 (Langerhans/mast cells) was performed. ANOVA plus Bonferroni’s tests were used for statistical analysis. Results. Twenty NCGS, 16 celiac disease, and 16 negative controls were selected. CD3 in NCGS were higher than negative controls and lower than celiac disease (18.5 ± 6.4, 11.9 ± 2.8, and 40.8 ± 8.1 IELs/100 enterocytes; p<0.001). CD4 were lower in NCGS than controls and celiac disease (31.0 ± 22.1, 72.5 ± 29.5, and 103.7 ± 15.7 cells/mm2; p<0.001). CD8 in NCGS were similar to negative controls, but lower than celiac disease (14.0 ± 7.4 and 34.0 ± 7.1 IELs/100 enterocytes, p<0.001). CD117 were higher in NCGS than celiac disease and negative controls (145.8 ± 49.9, 121.3 ± 13.1, and 113.5 ± 23.4 cells/mm2; p=0.009). Conclusions. The combination of CD4 and CD117, as well as IEL characterization, may be useful to support a clinical diagnosis of NCGS.http://dx.doi.org/10.1155/2017/5023680 |
| spellingShingle | Giuseppe Losurdo Domenico Piscitelli Federica Pezzuto Francesco Fortarezza Claudia Covelli Antonella Marra Andrea Iannone Annacinzia Amoruso Mariabeatrice Principi Enzo Ierardi Alfredo Di Leo T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity Gastroenterology Research and Practice |
| title | T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity |
| title_full | T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity |
| title_fullStr | T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity |
| title_full_unstemmed | T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity |
| title_short | T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity |
| title_sort | t helper lymphocyte and mast cell immunohistochemical pattern in nonceliac gluten sensitivity |
| url | http://dx.doi.org/10.1155/2017/5023680 |
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