Human Mature Adipocytes Express Albumin and This Expression Is Not Regulated by Inflammation
Aims. Our group investigated albumin gene expression in human adipocytes, its regulation by inflammation and the possible contribution of adipose tissue to albumin circulating levels. Methods. Both inflamed and healthy subjects provided adipose tissue samples. RT-PCR, Real-Time PCR, and Western Blot...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/236796 |
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| author | Maria Luisa Sirico Bruna Guida Alfredo Procino Andrea Pota Maurizio Sodo Giuseppe Grandaliano Simona Simone Giovanni Pertosa Eleonora Riccio Bruno Memoli |
| author_facet | Maria Luisa Sirico Bruna Guida Alfredo Procino Andrea Pota Maurizio Sodo Giuseppe Grandaliano Simona Simone Giovanni Pertosa Eleonora Riccio Bruno Memoli |
| author_sort | Maria Luisa Sirico |
| collection | DOAJ |
| description | Aims. Our group investigated albumin gene expression in human adipocytes, its regulation by inflammation and the possible contribution of adipose tissue to albumin circulating levels. Methods. Both inflamed and healthy subjects provided adipose tissue samples. RT-PCR, Real-Time PCR, and Western Blot analysis on homogenates of adipocytes and pre-adipocytes were performed. In sixty-three healthy subjects and fifty-four micro-inflamed end stage renal disease (ESRD) patients circulating levels of albumin were measured by nephelometry; all subjects were also evaluated for body composition, calculated from bioelectrical measurements and an thropometric data. Results. A clear gene expression of albumin was showed in pre-adipocytes and, for the first time, in mature adipocytes. Albumin gene expression resulted significantly higher in pre-adipocytes than in adipocytes. No significant difference in albumin gene expression was showed between healthy controls and inflamed patients. A significant negative correlation was observed between albumin levels and fat mass in both healthy subjects and inflamed ESRD patients. Conclusions. In the present study we found first time evidence that human adipocytes express albumin. Our results also showed that systemic inflammation does not modulate albumin gene expression. The negative correlation between albumin and fat mass seems to exclude a significant contributing role of adipocyte in plasma albumin. |
| format | Article |
| id | doaj-art-db875ed9e1ae42fa9c35bf899c2cf822 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-db875ed9e1ae42fa9c35bf899c2cf8222025-02-03T05:57:10ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/236796236796Human Mature Adipocytes Express Albumin and This Expression Is Not Regulated by InflammationMaria Luisa Sirico0Bruna Guida1Alfredo Procino2Andrea Pota3Maurizio Sodo4Giuseppe Grandaliano5Simona Simone6Giovanni Pertosa7Eleonora Riccio8Bruno Memoli9Department of Nephrology, University Federico II of Naples, 80131 Naples, ItalyNutrition Section, Department of Neuroscience, University Federico II of Naples, 80131 Naples, ItalyDepartment of Nephrology, University Federico II of Naples, 80131 Naples, ItalyDepartment of Nephrology, University Federico II of Naples, 80131 Naples, ItalyDepartment of General Surgery, University Federico II of Naples, 80131 Naples, ItalyDepartment of Nephrology, University of Bari, 70124 Bari, ItalyDepartment of Nephrology, University of Bari, 70124 Bari, ItalyDepartment of Nephrology, University of Bari, 70124 Bari, ItalyDepartment of Nephrology, University Federico II of Naples, 80131 Naples, ItalyDepartment of Nephrology, University Federico II of Naples, 80131 Naples, ItalyAims. Our group investigated albumin gene expression in human adipocytes, its regulation by inflammation and the possible contribution of adipose tissue to albumin circulating levels. Methods. Both inflamed and healthy subjects provided adipose tissue samples. RT-PCR, Real-Time PCR, and Western Blot analysis on homogenates of adipocytes and pre-adipocytes were performed. In sixty-three healthy subjects and fifty-four micro-inflamed end stage renal disease (ESRD) patients circulating levels of albumin were measured by nephelometry; all subjects were also evaluated for body composition, calculated from bioelectrical measurements and an thropometric data. Results. A clear gene expression of albumin was showed in pre-adipocytes and, for the first time, in mature adipocytes. Albumin gene expression resulted significantly higher in pre-adipocytes than in adipocytes. No significant difference in albumin gene expression was showed between healthy controls and inflamed patients. A significant negative correlation was observed between albumin levels and fat mass in both healthy subjects and inflamed ESRD patients. Conclusions. In the present study we found first time evidence that human adipocytes express albumin. Our results also showed that systemic inflammation does not modulate albumin gene expression. The negative correlation between albumin and fat mass seems to exclude a significant contributing role of adipocyte in plasma albumin.http://dx.doi.org/10.1155/2012/236796 |
| spellingShingle | Maria Luisa Sirico Bruna Guida Alfredo Procino Andrea Pota Maurizio Sodo Giuseppe Grandaliano Simona Simone Giovanni Pertosa Eleonora Riccio Bruno Memoli Human Mature Adipocytes Express Albumin and This Expression Is Not Regulated by Inflammation Mediators of Inflammation |
| title | Human Mature Adipocytes Express Albumin and This Expression Is Not Regulated by Inflammation |
| title_full | Human Mature Adipocytes Express Albumin and This Expression Is Not Regulated by Inflammation |
| title_fullStr | Human Mature Adipocytes Express Albumin and This Expression Is Not Regulated by Inflammation |
| title_full_unstemmed | Human Mature Adipocytes Express Albumin and This Expression Is Not Regulated by Inflammation |
| title_short | Human Mature Adipocytes Express Albumin and This Expression Is Not Regulated by Inflammation |
| title_sort | human mature adipocytes express albumin and this expression is not regulated by inflammation |
| url | http://dx.doi.org/10.1155/2012/236796 |
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