Screening of Potentially Probiotic Lactobacillus Possessing Surface Layer Protein from Iranian Traditional Dairy Products
One of the most commonly observed surface structures on the prokaryotic cell envelopes is monomolecular crystalline array of proteinaceous subunits termed Surface Layers or S-layers. Due their self assembly ability and the highly ordered, regular structure down to the nanometer scale, S-layers were...
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University of Tehran
2016-11-01
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| Series: | Journal of Sciences, Islamic Republic of Iran |
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| Online Access: | https://jsciences.ut.ac.ir/article_59336_71b67bf75e223a77f1a12baf4062dd2b.pdf |
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| author | E. Mobarak Qamsari R. Kasra Kermanshahi M. Erfan P. Ghadam |
| author_facet | E. Mobarak Qamsari R. Kasra Kermanshahi M. Erfan P. Ghadam |
| author_sort | E. Mobarak Qamsari |
| collection | DOAJ |
| description | One of the most commonly observed surface structures on the prokaryotic cell envelopes is monomolecular crystalline array of proteinaceous subunits termed Surface Layers or S-layers. Due their self assembly ability and the highly ordered, regular structure down to the nanometer scale, S-layers were demonstrated to possess a great potential for nanobiotechnological applications. Some probiotic bacteria, such as Lactobacillus spp. have been found to possess S-layers. In this perspective, the objectives of this study were isolation and identification of Lactobacillus species from traditional dairy products carrying surface (S) layer protein and investigation of important prerequisite of probiotic interest, such as the capability to survive at low pH and in presence of bile salts. The protein profile of intact Lactobacillus isolates was analyzed in SDS-PAGE. The protein bands of 45–60 kDa were present in two of the 25 isolates, suggesting the presence of S-layer proteins in these strains. The above mentioned proteins were recovered in the guanidine hydrochloride extracts. After dialysis, the extracted proteins showed a significant band of apparent molecular mass of 50–60 kDa in SDS-PAGE. The strains possessing S-layers protein was identified both biochemically and by 16S rRNA sequencing. The 16S rRNA gene sequence analysis revealed that two isolates exhibited maximum similarity with the 16S rRNA sequence of Lactobacillus brevis spp. These two isolates were resistant to acid and bile in which their viability in acidic condition were between 51.6% and 77.8% and bile resistance of two isolates was approximately 99% after 24 h. |
| format | Article |
| id | doaj-art-db81cb86f9804cc8bd6b10ab0ac848c6 |
| institution | OA Journals |
| issn | 1016-1104 2345-6914 |
| language | English |
| publishDate | 2016-11-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Journal of Sciences, Islamic Republic of Iran |
| spelling | doaj-art-db81cb86f9804cc8bd6b10ab0ac848c62025-08-20T01:53:09ZengUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-11042345-69142016-11-0127430531259336Screening of Potentially Probiotic Lactobacillus Possessing Surface Layer Protein from Iranian Traditional Dairy ProductsE. Mobarak Qamsari0R. Kasra Kermanshahi1M. Erfan2P. Ghadam3Department of Microbiology, Faculty of Biology, Alzahra University, Tehran, Islamic Republic of IranDepartment of Microbiology, Faculty of Biology, Alzahra University, Tehran, Islamic Republic of IranDepartment of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of IranDepartment of Biotechnology, Faculty of Biology, Alzahra University, Tehran, Islamic Republic of IranOne of the most commonly observed surface structures on the prokaryotic cell envelopes is monomolecular crystalline array of proteinaceous subunits termed Surface Layers or S-layers. Due their self assembly ability and the highly ordered, regular structure down to the nanometer scale, S-layers were demonstrated to possess a great potential for nanobiotechnological applications. Some probiotic bacteria, such as Lactobacillus spp. have been found to possess S-layers. In this perspective, the objectives of this study were isolation and identification of Lactobacillus species from traditional dairy products carrying surface (S) layer protein and investigation of important prerequisite of probiotic interest, such as the capability to survive at low pH and in presence of bile salts. The protein profile of intact Lactobacillus isolates was analyzed in SDS-PAGE. The protein bands of 45–60 kDa were present in two of the 25 isolates, suggesting the presence of S-layer proteins in these strains. The above mentioned proteins were recovered in the guanidine hydrochloride extracts. After dialysis, the extracted proteins showed a significant band of apparent molecular mass of 50–60 kDa in SDS-PAGE. The strains possessing S-layers protein was identified both biochemically and by 16S rRNA sequencing. The 16S rRNA gene sequence analysis revealed that two isolates exhibited maximum similarity with the 16S rRNA sequence of Lactobacillus brevis spp. These two isolates were resistant to acid and bile in which their viability in acidic condition were between 51.6% and 77.8% and bile resistance of two isolates was approximately 99% after 24 h.https://jsciences.ut.ac.ir/article_59336_71b67bf75e223a77f1a12baf4062dd2b.pdfdairy productslactobacillus spps-layerprobiotic |
| spellingShingle | E. Mobarak Qamsari R. Kasra Kermanshahi M. Erfan P. Ghadam Screening of Potentially Probiotic Lactobacillus Possessing Surface Layer Protein from Iranian Traditional Dairy Products Journal of Sciences, Islamic Republic of Iran dairy products lactobacillus spp s-layer probiotic |
| title | Screening of Potentially Probiotic Lactobacillus Possessing Surface Layer Protein from Iranian Traditional Dairy Products |
| title_full | Screening of Potentially Probiotic Lactobacillus Possessing Surface Layer Protein from Iranian Traditional Dairy Products |
| title_fullStr | Screening of Potentially Probiotic Lactobacillus Possessing Surface Layer Protein from Iranian Traditional Dairy Products |
| title_full_unstemmed | Screening of Potentially Probiotic Lactobacillus Possessing Surface Layer Protein from Iranian Traditional Dairy Products |
| title_short | Screening of Potentially Probiotic Lactobacillus Possessing Surface Layer Protein from Iranian Traditional Dairy Products |
| title_sort | screening of potentially probiotic lactobacillus possessing surface layer protein from iranian traditional dairy products |
| topic | dairy products lactobacillus spp s-layer probiotic |
| url | https://jsciences.ut.ac.ir/article_59336_71b67bf75e223a77f1a12baf4062dd2b.pdf |
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