Impact of Brain derived Neurotrophic factor gene polymorphism on its peripheral levels in schizophrenic patients
Objective: To determine serum levels of brain-derived neurotrophic factor and its polymorphism rs12291063 in schizophrenic patients. Method: The case-control study was conducted from January 1, 2020, to May 15, 2021, at Dr Abdul Qadeer Khan Institute of Behavioural Sciences, Dow University of Hea...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pakistan Medical Association
2024-02-01
|
| Series: | Journal of the Pakistan Medical Association |
| Online Access: | https://jpma.org.pk/index.php/public_html/article/view/20240 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850098758996459520 |
|---|---|
| author | Irshad Bibi Farina Hanif Quratulain Amir Washdev Washdev Mohammad Furqan Bari |
| author_facet | Irshad Bibi Farina Hanif Quratulain Amir Washdev Washdev Mohammad Furqan Bari |
| author_sort | Irshad Bibi |
| collection | DOAJ |
| description |
Objective: To determine serum levels of brain-derived neurotrophic factor and its polymorphism rs12291063 in schizophrenic patients.
Method: The case-control study was conducted from January 1, 2020, to May 15, 2021, at Dr Abdul Qadeer Khan Institute of Behavioural Sciences, Dow University of Health Sciences, Karachi, and comprised schizophrenia cases aged 14-60 years who were diagnosed using Diagnostic and Statistical Manual of Mental Disorders-V criteria, and healthy controls without any psychiatric illness. Positive and negative syndrome scale score was used to assess disease severity. The genomic deoxyribonucleic acid of the subjects was isolated from peripheral blood, followed by polymerase chain reaction, gel electrophoresis and sequencing of the amplicons. The sequences were analysed using MEGA X software for genotyping. Serum brain-derived neurotrophic factor levels were assessed using enzyme-linked immunosorbent assay. Data was analysed using SPSS 21.
Results: Of the 100 subjects, 50(50%) were cases; 36(72%) males and 14(28%) females (p<0.05) with mean age 34.34±10.32 years. There were 50(50%) controls; 32(64%) males and 18(36%) females (p=0.391) with mean age 30.886±8.88 years. Among the cases, the mean age at schizophrenia diagnosis was 25.14±9.54 years, and there was a significant association with positive family history for psychiatric disorders (p<0.05). Sequencing revealed no T>C substitution. Serum brain-derived neurotrophic factor levels were significantly higher in cases compared to controls (p<0.001). There was a weak negative correlation between brain-derived neurotrophic factor levels and positive and negative syndrome scale score (p<0.05).
Conclusion: Higher brain-derived neurotrophic factor levels were found to be associated with schizophrenia, while no association of rs12291063 T>C was found with schizophrenia.
Key Words: Brain derived neurotrphic factor, genotyping, Schizophrenia, Mental Disorders.
|
| format | Article |
| id | doaj-art-db75723cf1ed4326b6000759e6a77458 |
| institution | DOAJ |
| issn | 0030-9982 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Pakistan Medical Association |
| record_format | Article |
| series | Journal of the Pakistan Medical Association |
| spelling | doaj-art-db75723cf1ed4326b6000759e6a774582025-08-20T02:40:39ZengPakistan Medical AssociationJournal of the Pakistan Medical Association0030-99822024-02-0174210.47391/JPMA-DUHS-S05Impact of Brain derived Neurotrophic factor gene polymorphism on its peripheral levels in schizophrenic patientsIrshad Bibi0Farina Hanif1Quratulain Amir2Washdev Washdev3Mohammad Furqan Bari4Institute of Biomedical Sciences, Dow International Medical College, Dow University of Health Sciences, Karachi, Pakistan.Department of Biochemistry, Dow International Medical College, Dow University of Health Sciences, Karachi, Pakistan.Institute of Medical Technology, Dow University of Health Sciences, Karachi, Pakistan.Dr. Abdul Qadeer Khan Institute of Behavioral Sciences, Dow University of Health Sciences, Karachi, Pakistan.Dr. Ishrat-Ul-Ebad Khan Institute of Oral Health Sciences, Dow University of Health Sciences, Karachi, Pakistan Objective: To determine serum levels of brain-derived neurotrophic factor and its polymorphism rs12291063 in schizophrenic patients. Method: The case-control study was conducted from January 1, 2020, to May 15, 2021, at Dr Abdul Qadeer Khan Institute of Behavioural Sciences, Dow University of Health Sciences, Karachi, and comprised schizophrenia cases aged 14-60 years who were diagnosed using Diagnostic and Statistical Manual of Mental Disorders-V criteria, and healthy controls without any psychiatric illness. Positive and negative syndrome scale score was used to assess disease severity. The genomic deoxyribonucleic acid of the subjects was isolated from peripheral blood, followed by polymerase chain reaction, gel electrophoresis and sequencing of the amplicons. The sequences were analysed using MEGA X software for genotyping. Serum brain-derived neurotrophic factor levels were assessed using enzyme-linked immunosorbent assay. Data was analysed using SPSS 21. Results: Of the 100 subjects, 50(50%) were cases; 36(72%) males and 14(28%) females (p<0.05) with mean age 34.34±10.32 years. There were 50(50%) controls; 32(64%) males and 18(36%) females (p=0.391) with mean age 30.886±8.88 years. Among the cases, the mean age at schizophrenia diagnosis was 25.14±9.54 years, and there was a significant association with positive family history for psychiatric disorders (p<0.05). Sequencing revealed no T>C substitution. Serum brain-derived neurotrophic factor levels were significantly higher in cases compared to controls (p<0.001). There was a weak negative correlation between brain-derived neurotrophic factor levels and positive and negative syndrome scale score (p<0.05). Conclusion: Higher brain-derived neurotrophic factor levels were found to be associated with schizophrenia, while no association of rs12291063 T>C was found with schizophrenia. Key Words: Brain derived neurotrphic factor, genotyping, Schizophrenia, Mental Disorders. https://jpma.org.pk/index.php/public_html/article/view/20240 |
| spellingShingle | Irshad Bibi Farina Hanif Quratulain Amir Washdev Washdev Mohammad Furqan Bari Impact of Brain derived Neurotrophic factor gene polymorphism on its peripheral levels in schizophrenic patients Journal of the Pakistan Medical Association |
| title | Impact of Brain derived Neurotrophic factor gene polymorphism on its peripheral levels in schizophrenic patients |
| title_full | Impact of Brain derived Neurotrophic factor gene polymorphism on its peripheral levels in schizophrenic patients |
| title_fullStr | Impact of Brain derived Neurotrophic factor gene polymorphism on its peripheral levels in schizophrenic patients |
| title_full_unstemmed | Impact of Brain derived Neurotrophic factor gene polymorphism on its peripheral levels in schizophrenic patients |
| title_short | Impact of Brain derived Neurotrophic factor gene polymorphism on its peripheral levels in schizophrenic patients |
| title_sort | impact of brain derived neurotrophic factor gene polymorphism on its peripheral levels in schizophrenic patients |
| url | https://jpma.org.pk/index.php/public_html/article/view/20240 |
| work_keys_str_mv | AT irshadbibi impactofbrainderivedneurotrophicfactorgenepolymorphismonitsperipherallevelsinschizophrenicpatients AT farinahanif impactofbrainderivedneurotrophicfactorgenepolymorphismonitsperipherallevelsinschizophrenicpatients AT quratulainamir impactofbrainderivedneurotrophicfactorgenepolymorphismonitsperipherallevelsinschizophrenicpatients AT washdevwashdev impactofbrainderivedneurotrophicfactorgenepolymorphismonitsperipherallevelsinschizophrenicpatients AT mohammadfurqanbari impactofbrainderivedneurotrophicfactorgenepolymorphismonitsperipherallevelsinschizophrenicpatients |