Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement
A series of biodegradable nanoparticle-based drug delivery systems have been designed utilizing poly(β-amino ester)-guanidine-phenylboronic acid (PBAE-G) polymers. In this study, a novel Lentinan-Functionalized PBAE-G-nanodiamond system was developed to carry ovalbumin (LNT-PBAE-G-ND@OVA). The impac...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Journal of Pharmaceutical Analysis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177923002952 |
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| author | Zhiqiang Zhang Li Wang Xia Ma Hui Wang |
| author_facet | Zhiqiang Zhang Li Wang Xia Ma Hui Wang |
| author_sort | Zhiqiang Zhang |
| collection | DOAJ |
| description | A series of biodegradable nanoparticle-based drug delivery systems have been designed utilizing poly(β-amino ester)-guanidine-phenylboronic acid (PBAE-G) polymers. In this study, a novel Lentinan-Functionalized PBAE-G-nanodiamond system was developed to carry ovalbumin (LNT-PBAE-G-ND@OVA). The impact of this drug delivery system on the activation and maturation of macrophages was then assessed. Furthermore, LNT-PBAE-G-ND@OVA induced potent antibody response and showed no obvious toxicity in vitro and in vivo. Moreover, treatment with LNT-PBAE-G-ND@OVA was sufficient to alter the expression of genes associated with the cGAS-STING pathway, and the LNT-PBAE-G-ND@OVA induced upregulation of costimulatory molecules. LNT-PBAE-G-ND@OVA treatment was sufficient to induce macrophage activation through a complex mechanism in which cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) synthase (cGAS)-stimulator of interferon genes (STING) signaling plays an integral role. |
| format | Article |
| id | doaj-art-db44ddc32b0043bf91ceb91ee1bf0a69 |
| institution | Kabale University |
| issn | 2095-1779 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj-art-db44ddc32b0043bf91ceb91ee1bf0a692025-01-30T05:13:56ZengElsevierJournal of Pharmaceutical Analysis2095-17792024-12-011412100922Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancementZhiqiang Zhang0Li Wang1Xia Ma2Hui Wang3College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450045, ChinaDepartment of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou, 450046, ChinaCollege of Animal Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China; Corresponding author.College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450045, China; Corresponding author.A series of biodegradable nanoparticle-based drug delivery systems have been designed utilizing poly(β-amino ester)-guanidine-phenylboronic acid (PBAE-G) polymers. In this study, a novel Lentinan-Functionalized PBAE-G-nanodiamond system was developed to carry ovalbumin (LNT-PBAE-G-ND@OVA). The impact of this drug delivery system on the activation and maturation of macrophages was then assessed. Furthermore, LNT-PBAE-G-ND@OVA induced potent antibody response and showed no obvious toxicity in vitro and in vivo. Moreover, treatment with LNT-PBAE-G-ND@OVA was sufficient to alter the expression of genes associated with the cGAS-STING pathway, and the LNT-PBAE-G-ND@OVA induced upregulation of costimulatory molecules. LNT-PBAE-G-ND@OVA treatment was sufficient to induce macrophage activation through a complex mechanism in which cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) synthase (cGAS)-stimulator of interferon genes (STING) signaling plays an integral role.http://www.sciencedirect.com/science/article/pii/S2095177923002952LNT-PBAE-G-ND@OVAMolecular mechanismscGAS-STING signaling |
| spellingShingle | Zhiqiang Zhang Li Wang Xia Ma Hui Wang Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement Journal of Pharmaceutical Analysis LNT-PBAE-G-ND@OVA Molecular mechanisms cGAS-STING signaling |
| title | Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement |
| title_full | Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement |
| title_fullStr | Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement |
| title_full_unstemmed | Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement |
| title_short | Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement |
| title_sort | lentinan functionalized pbae g nanodiamonds as an adjuvant to induce cgas sting pathway mediated macrophage activation and immune enhancement |
| topic | LNT-PBAE-G-ND@OVA Molecular mechanisms cGAS-STING signaling |
| url | http://www.sciencedirect.com/science/article/pii/S2095177923002952 |
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