The COPII Transport Complex Participates in HPV16 Infection
Human papillomavirus (HPV) 16 is transported in a retrograde fashion from the cell surface to the Golgi apparatus. Prior to mitosis, the virus loses association with the Golgi and, following nuclear envelope breakdown, is found associated with the condensed mitotic chromatin. The intervening steps h...
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2025-04-01
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| Online Access: | https://www.mdpi.com/1999-4915/17/5/616 |
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| author | Patricia M. Day Cynthia D. Thompson Andrea S. Weisberg John T. Schiller |
| author_facet | Patricia M. Day Cynthia D. Thompson Andrea S. Weisberg John T. Schiller |
| author_sort | Patricia M. Day |
| collection | DOAJ |
| description | Human papillomavirus (HPV) 16 is transported in a retrograde fashion from the cell surface to the Golgi apparatus. Prior to mitosis, the virus loses association with the Golgi and, following nuclear envelope breakdown, is found associated with the condensed mitotic chromatin. The intervening steps have not been well defined. It was previously demonstrated that the virus is transported to the mitotic chromosomes in vesicles. Here, we describe the role of the endoplasmic reticulum (ER) in the post-Golgi trafficking and the importance of the ER-generated coat protein complex II (COPII) anterograde trafficking pathway in HPV infection. HPV pseudovirus (PsV) colocalized with COPII components and silencing of this pathway inhibited HPV infection. Additionally, the inner COPII coat protein, Sec24b, could be biochemically isolated in association with HPV capsid proteins. This study provides insight into the mechanism of post-Golgi HPV trafficking. |
| format | Article |
| id | doaj-art-db2bc04dbfc7407eaa83887a2af44929 |
| institution | OA Journals |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-db2bc04dbfc7407eaa83887a2af449292025-08-20T01:56:42ZengMDPI AGViruses1999-49152025-04-0117561610.3390/v17050616The COPII Transport Complex Participates in HPV16 InfectionPatricia M. Day0Cynthia D. Thompson1Andrea S. Weisberg2John T. Schiller3Laboratory of Cellular Oncology, NCI, NIH, Bethesda, MD 20892, USALaboratory of Cellular Oncology, NCI, NIH, Bethesda, MD 20892, USALaboratory of Viral Diseases, NIAID, NIH, Bethesda, MD 20892, USALaboratory of Cellular Oncology, NCI, NIH, Bethesda, MD 20892, USAHuman papillomavirus (HPV) 16 is transported in a retrograde fashion from the cell surface to the Golgi apparatus. Prior to mitosis, the virus loses association with the Golgi and, following nuclear envelope breakdown, is found associated with the condensed mitotic chromatin. The intervening steps have not been well defined. It was previously demonstrated that the virus is transported to the mitotic chromosomes in vesicles. Here, we describe the role of the endoplasmic reticulum (ER) in the post-Golgi trafficking and the importance of the ER-generated coat protein complex II (COPII) anterograde trafficking pathway in HPV infection. HPV pseudovirus (PsV) colocalized with COPII components and silencing of this pathway inhibited HPV infection. Additionally, the inner COPII coat protein, Sec24b, could be biochemically isolated in association with HPV capsid proteins. This study provides insight into the mechanism of post-Golgi HPV trafficking.https://www.mdpi.com/1999-4915/17/5/616HPVpapillomaviruspseudovirustraffickingmitosisGolgi |
| spellingShingle | Patricia M. Day Cynthia D. Thompson Andrea S. Weisberg John T. Schiller The COPII Transport Complex Participates in HPV16 Infection Viruses HPV papillomavirus pseudovirus trafficking mitosis Golgi |
| title | The COPII Transport Complex Participates in HPV16 Infection |
| title_full | The COPII Transport Complex Participates in HPV16 Infection |
| title_fullStr | The COPII Transport Complex Participates in HPV16 Infection |
| title_full_unstemmed | The COPII Transport Complex Participates in HPV16 Infection |
| title_short | The COPII Transport Complex Participates in HPV16 Infection |
| title_sort | copii transport complex participates in hpv16 infection |
| topic | HPV papillomavirus pseudovirus trafficking mitosis Golgi |
| url | https://www.mdpi.com/1999-4915/17/5/616 |
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