Characterization of two neutralizing monoclonal antibodies with conformational epitopes against porcine deltacoronavirus
Abstract Porcine deltacoronavirus (PDCoV) is a globally distributed swine enteropathogenic virus that emerged in the last decade. A recent report of PDCoV infection in Haitian children also highlights potential public health implications. In this study, two monoclonal antibodies (mAbs), 1C2 and 5H5,...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Animal Diseases |
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| Online Access: | https://doi.org/10.1186/s44149-025-00156-z |
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| author | Wan Lu Hongtao Cao Yongle Yang Yangyang Sun Dong Yang Priscilla F. Gerber Xiangdong Li Yaowei Huang Bin Wang |
| author_facet | Wan Lu Hongtao Cao Yongle Yang Yangyang Sun Dong Yang Priscilla F. Gerber Xiangdong Li Yaowei Huang Bin Wang |
| author_sort | Wan Lu |
| collection | DOAJ |
| description | Abstract Porcine deltacoronavirus (PDCoV) is a globally distributed swine enteropathogenic virus that emerged in the last decade. A recent report of PDCoV infection in Haitian children also highlights potential public health implications. In this study, two monoclonal antibodies (mAbs), 1C2 and 5H5, were generated and showed high specificity for the PDCoV S protein. Both mAbs displayed high-titer neutralizing capabilities, suggesting their potential for passive immunotherapy. Epitope mapping revealed that the mAbs likely recognized conformational epitopes in the S1 subunit domains A and B of the native S protein, thereby blocking the interaction between the S1 receptor-binding domain and the cellular receptor, which could inhibit viral entry into host cells. This study offers new biological tools for PDCoV detection and lays the groundwork for the future development of porcine-specific antibodies for the prevention and treatment of PDCoV in piglets. |
| format | Article |
| id | doaj-art-db1b1293401f4d609d688cdabec26e0e |
| institution | Kabale University |
| issn | 2731-0442 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Animal Diseases |
| spelling | doaj-art-db1b1293401f4d609d688cdabec26e0e2025-01-26T12:22:35ZengBMCAnimal Diseases2731-04422025-01-015111010.1186/s44149-025-00156-zCharacterization of two neutralizing monoclonal antibodies with conformational epitopes against porcine deltacoronavirusWan Lu0Hongtao Cao1Yongle Yang2Yangyang Sun3Dong Yang4Priscilla F. Gerber5Xiangdong Li6Yaowei Huang7Bin Wang8Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural UniversityXianghu LaboratoryXianghu LaboratoryXianghu LaboratoryXianghu LaboratoryDepartment of Infectious Diseases and Public Health, City University of Hong KongJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityGuangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural UniversityGuangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural UniversityAbstract Porcine deltacoronavirus (PDCoV) is a globally distributed swine enteropathogenic virus that emerged in the last decade. A recent report of PDCoV infection in Haitian children also highlights potential public health implications. In this study, two monoclonal antibodies (mAbs), 1C2 and 5H5, were generated and showed high specificity for the PDCoV S protein. Both mAbs displayed high-titer neutralizing capabilities, suggesting their potential for passive immunotherapy. Epitope mapping revealed that the mAbs likely recognized conformational epitopes in the S1 subunit domains A and B of the native S protein, thereby blocking the interaction between the S1 receptor-binding domain and the cellular receptor, which could inhibit viral entry into host cells. This study offers new biological tools for PDCoV detection and lays the groundwork for the future development of porcine-specific antibodies for the prevention and treatment of PDCoV in piglets.https://doi.org/10.1186/s44149-025-00156-zPorcine deltacoronavirus (PDCoV)SpikeNeutralizing antibodyMonoclonal antibodyEpitope |
| spellingShingle | Wan Lu Hongtao Cao Yongle Yang Yangyang Sun Dong Yang Priscilla F. Gerber Xiangdong Li Yaowei Huang Bin Wang Characterization of two neutralizing monoclonal antibodies with conformational epitopes against porcine deltacoronavirus Animal Diseases Porcine deltacoronavirus (PDCoV) Spike Neutralizing antibody Monoclonal antibody Epitope |
| title | Characterization of two neutralizing monoclonal antibodies with conformational epitopes against porcine deltacoronavirus |
| title_full | Characterization of two neutralizing monoclonal antibodies with conformational epitopes against porcine deltacoronavirus |
| title_fullStr | Characterization of two neutralizing monoclonal antibodies with conformational epitopes against porcine deltacoronavirus |
| title_full_unstemmed | Characterization of two neutralizing monoclonal antibodies with conformational epitopes against porcine deltacoronavirus |
| title_short | Characterization of two neutralizing monoclonal antibodies with conformational epitopes against porcine deltacoronavirus |
| title_sort | characterization of two neutralizing monoclonal antibodies with conformational epitopes against porcine deltacoronavirus |
| topic | Porcine deltacoronavirus (PDCoV) Spike Neutralizing antibody Monoclonal antibody Epitope |
| url | https://doi.org/10.1186/s44149-025-00156-z |
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