Expression of p120-Catenin Isoforms Correlates with Genomic and Transcriptional Phenotype of Breast Cancer Cell Lines
Background: P120-catenin is a member of the Armadillo protein family, which is involved in intercellular adhesion and cell signalling. It directly interacts with the classical cadherins juxtamembrane domain and contributes for both junction formation and its disassembly. Accumulating evidences indic...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2007-01-01
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| Series: | Cellular Oncology |
| Online Access: | http://dx.doi.org/10.1155/2007/395913 |
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| _version_ | 1832548154530070528 |
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| author | Joana Paredes Ana Luísa Correia Ana Sofia Ribeiro Fernando Schmitt |
| author_facet | Joana Paredes Ana Luísa Correia Ana Sofia Ribeiro Fernando Schmitt |
| author_sort | Joana Paredes |
| collection | DOAJ |
| description | Background: P120-catenin is a member of the Armadillo protein family, which is involved in intercellular adhesion and cell signalling. It directly interacts with the classical cadherins juxtamembrane domain and contributes for both junction formation and its disassembly. Accumulating evidences indicate that p120-catenin is important in tumour formation and progression, although the role of their multiple spliced isoforms in the regulation of cadherin-mediated adhesion of malignant cells is still not well understood. We investigated the expression of p120-catenin isoforms in a collection of breast cancer cell lines with distinct molecular profiles and expressing different cadherins. Methods: We assessed the expression by RT-PCR and Western-blotting analysis. Results: We observed that the expression of p120-catenin isoforms was associated with the genomic and transcriptional phenotype of breast cancer cells. Besides, the recruitment of p120-catenin isoforms was not apparently related with the particular expression of E-, P- or N-cadherin. Conclusion: We demonstrate that mammary tumour cells exhibit a characteristic p120-catenin isoform expression profile, depending from their specific genomic and transcriptional properties. These particular expression patterns, combined with other regulatory proteins and in a specific cellular context, may explain how p120-catenin can either contribute to strength intercellular adhesions or instead to promote cell motility. |
| format | Article |
| id | doaj-art-db072e722a4a467e9951fa0280db8cce |
| institution | Kabale University |
| issn | 1570-5870 1875-8606 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cellular Oncology |
| spelling | doaj-art-db072e722a4a467e9951fa0280db8cce2025-02-03T06:42:06ZengWileyCellular Oncology1570-58701875-86062007-01-0129646747610.1155/2007/395913Expression of p120-Catenin Isoforms Correlates with Genomic and Transcriptional Phenotype of Breast Cancer Cell LinesJoana Paredes0Ana Luísa Correia1Ana Sofia Ribeiro2Fernando Schmitt3Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, PortugalLife and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, PortugalInstitute of Molecular Pathology and Immunology of Porto University (IPATIMUP), Porto, PortugalLife and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, PortugalBackground: P120-catenin is a member of the Armadillo protein family, which is involved in intercellular adhesion and cell signalling. It directly interacts with the classical cadherins juxtamembrane domain and contributes for both junction formation and its disassembly. Accumulating evidences indicate that p120-catenin is important in tumour formation and progression, although the role of their multiple spliced isoforms in the regulation of cadherin-mediated adhesion of malignant cells is still not well understood. We investigated the expression of p120-catenin isoforms in a collection of breast cancer cell lines with distinct molecular profiles and expressing different cadherins. Methods: We assessed the expression by RT-PCR and Western-blotting analysis. Results: We observed that the expression of p120-catenin isoforms was associated with the genomic and transcriptional phenotype of breast cancer cells. Besides, the recruitment of p120-catenin isoforms was not apparently related with the particular expression of E-, P- or N-cadherin. Conclusion: We demonstrate that mammary tumour cells exhibit a characteristic p120-catenin isoform expression profile, depending from their specific genomic and transcriptional properties. These particular expression patterns, combined with other regulatory proteins and in a specific cellular context, may explain how p120-catenin can either contribute to strength intercellular adhesions or instead to promote cell motility.http://dx.doi.org/10.1155/2007/395913 |
| spellingShingle | Joana Paredes Ana Luísa Correia Ana Sofia Ribeiro Fernando Schmitt Expression of p120-Catenin Isoforms Correlates with Genomic and Transcriptional Phenotype of Breast Cancer Cell Lines Cellular Oncology |
| title | Expression of p120-Catenin Isoforms Correlates with Genomic and Transcriptional Phenotype of Breast Cancer Cell Lines |
| title_full | Expression of p120-Catenin Isoforms Correlates with Genomic and Transcriptional Phenotype of Breast Cancer Cell Lines |
| title_fullStr | Expression of p120-Catenin Isoforms Correlates with Genomic and Transcriptional Phenotype of Breast Cancer Cell Lines |
| title_full_unstemmed | Expression of p120-Catenin Isoforms Correlates with Genomic and Transcriptional Phenotype of Breast Cancer Cell Lines |
| title_short | Expression of p120-Catenin Isoforms Correlates with Genomic and Transcriptional Phenotype of Breast Cancer Cell Lines |
| title_sort | expression of p120 catenin isoforms correlates with genomic and transcriptional phenotype of breast cancer cell lines |
| url | http://dx.doi.org/10.1155/2007/395913 |
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