The comparison of biodistribution of glutathione PEGylated nanoliposomal doxorubicin formulations prepared by pre‐insertion and post‐insertion methods for brain delivery in normal mice
Abstract Several obstacles limit the efficacy of brain tumour treatment, most notably the blood‐brain barrier (BBB), which prevents the brain uptake of the majority of accessible medicines due to tight junctions. The presence of glutathione (GSH) receptors on the BBB surface has been demonstrated in...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-04-01
|
| Series: | IET Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1049/nbt2.12111 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546700639600640 |
|---|---|
| author | Amin Mehrabian Saba Dadpour Mohammad Mashreghi Javad Zarqi Anis Askarizadeh Ali Badiee Leila Arabi Seyedeh Alia Moosavian Mahmoud Reza Jaafari |
| author_facet | Amin Mehrabian Saba Dadpour Mohammad Mashreghi Javad Zarqi Anis Askarizadeh Ali Badiee Leila Arabi Seyedeh Alia Moosavian Mahmoud Reza Jaafari |
| author_sort | Amin Mehrabian |
| collection | DOAJ |
| description | Abstract Several obstacles limit the efficacy of brain tumour treatment, most notably the blood‐brain barrier (BBB), which prevents the brain uptake of the majority of accessible medicines due to tight junctions. The presence of glutathione (GSH) receptors on the BBB surface has been demonstrated in numerous papers; consequently, products containing glutathione as a targeting ligand coupled with nanoliposomes are used to enhance drug delivery across the BBB. Here, the 5% pre‐inserted PEG2000‐GSH PEGylated liposomal doxorubicin was conducted according to 2B3‐101 being tested in clinical trials. In addition, PEGylated nanoliposomal doxorubicin connected to the spacer‐GSH targeting ligand (GSGGCE) and the PEG3400 was conducted using post‐insertion method. Next, in vivo biodistribution of the produced formulations was tested on healthy mice to see if GSGGCE, as the targeted ligand, could cross the BBB compared to 5% pre‐inserted PEG2000‐GSH and Caelyx®. Compared to the pre‐inserted formulation and Caelyx®, the post‐inserted formulations' concentration was lower in the heart and higher in brain tissues, resulting in boosting the brain concentration of accumulated doxorubicin with fewer possible side effects, including cardiotoxicity. In comparison to the pre‐insertion procedure, the post‐insertion method is easier, faster, and more cost‐effective. Moreover, employing PEG3400 and the post‐insertion approach in the PEG3400‐GSGGCE liposomal formulations was found to be effective in crossing the BBB. |
| format | Article |
| id | doaj-art-daff2480ef8942baacd8e408881c310b |
| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2023-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj-art-daff2480ef8942baacd8e408881c310b2025-02-03T06:47:34ZengWileyIET Nanobiotechnology1751-87411751-875X2023-04-0117211212410.1049/nbt2.12111The comparison of biodistribution of glutathione PEGylated nanoliposomal doxorubicin formulations prepared by pre‐insertion and post‐insertion methods for brain delivery in normal miceAmin Mehrabian0Saba Dadpour1Mohammad Mashreghi2Javad Zarqi3Anis Askarizadeh4Ali Badiee5Leila Arabi6Seyedeh Alia Moosavian7Mahmoud Reza Jaafari8Department of Pharmaceutical Nanotechnology School of Pharmacy Mashhad University of Medical Sciences Mashhad IranDepartment of Pharmaceutical Nanotechnology School of Pharmacy Mashhad University of Medical Sciences Mashhad IranDepartment of Pharmaceutical Nanotechnology School of Pharmacy Mashhad University of Medical Sciences Mashhad IranDepartment of Pharmaceutical Nanotechnology School of Pharmacy Mashhad University of Medical Sciences Mashhad IranDepartment of Pharmaceutical Nanotechnology School of Pharmacy Mashhad University of Medical Sciences Mashhad IranDepartment of Pharmaceutical Nanotechnology School of Pharmacy Mashhad University of Medical Sciences Mashhad IranDepartment of Pharmaceutical Nanotechnology School of Pharmacy Mashhad University of Medical Sciences Mashhad IranDepartment of Pharmaceutical Nanotechnology School of Pharmacy Mashhad University of Medical Sciences Mashhad IranDepartment of Pharmaceutical Nanotechnology School of Pharmacy Mashhad University of Medical Sciences Mashhad IranAbstract Several obstacles limit the efficacy of brain tumour treatment, most notably the blood‐brain barrier (BBB), which prevents the brain uptake of the majority of accessible medicines due to tight junctions. The presence of glutathione (GSH) receptors on the BBB surface has been demonstrated in numerous papers; consequently, products containing glutathione as a targeting ligand coupled with nanoliposomes are used to enhance drug delivery across the BBB. Here, the 5% pre‐inserted PEG2000‐GSH PEGylated liposomal doxorubicin was conducted according to 2B3‐101 being tested in clinical trials. In addition, PEGylated nanoliposomal doxorubicin connected to the spacer‐GSH targeting ligand (GSGGCE) and the PEG3400 was conducted using post‐insertion method. Next, in vivo biodistribution of the produced formulations was tested on healthy mice to see if GSGGCE, as the targeted ligand, could cross the BBB compared to 5% pre‐inserted PEG2000‐GSH and Caelyx®. Compared to the pre‐inserted formulation and Caelyx®, the post‐inserted formulations' concentration was lower in the heart and higher in brain tissues, resulting in boosting the brain concentration of accumulated doxorubicin with fewer possible side effects, including cardiotoxicity. In comparison to the pre‐insertion procedure, the post‐insertion method is easier, faster, and more cost‐effective. Moreover, employing PEG3400 and the post‐insertion approach in the PEG3400‐GSGGCE liposomal formulations was found to be effective in crossing the BBB.https://doi.org/10.1049/nbt2.12111braindrug delivery systemsnanomedicinenanoparticles |
| spellingShingle | Amin Mehrabian Saba Dadpour Mohammad Mashreghi Javad Zarqi Anis Askarizadeh Ali Badiee Leila Arabi Seyedeh Alia Moosavian Mahmoud Reza Jaafari The comparison of biodistribution of glutathione PEGylated nanoliposomal doxorubicin formulations prepared by pre‐insertion and post‐insertion methods for brain delivery in normal mice IET Nanobiotechnology brain drug delivery systems nanomedicine nanoparticles |
| title | The comparison of biodistribution of glutathione PEGylated nanoliposomal doxorubicin formulations prepared by pre‐insertion and post‐insertion methods for brain delivery in normal mice |
| title_full | The comparison of biodistribution of glutathione PEGylated nanoliposomal doxorubicin formulations prepared by pre‐insertion and post‐insertion methods for brain delivery in normal mice |
| title_fullStr | The comparison of biodistribution of glutathione PEGylated nanoliposomal doxorubicin formulations prepared by pre‐insertion and post‐insertion methods for brain delivery in normal mice |
| title_full_unstemmed | The comparison of biodistribution of glutathione PEGylated nanoliposomal doxorubicin formulations prepared by pre‐insertion and post‐insertion methods for brain delivery in normal mice |
| title_short | The comparison of biodistribution of glutathione PEGylated nanoliposomal doxorubicin formulations prepared by pre‐insertion and post‐insertion methods for brain delivery in normal mice |
| title_sort | comparison of biodistribution of glutathione pegylated nanoliposomal doxorubicin formulations prepared by pre insertion and post insertion methods for brain delivery in normal mice |
| topic | brain drug delivery systems nanomedicine nanoparticles |
| url | https://doi.org/10.1049/nbt2.12111 |
| work_keys_str_mv | AT aminmehrabian thecomparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT sabadadpour thecomparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT mohammadmashreghi thecomparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT javadzarqi thecomparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT anisaskarizadeh thecomparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT alibadiee thecomparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT leilaarabi thecomparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT seyedehaliamoosavian thecomparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT mahmoudrezajaafari thecomparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT aminmehrabian comparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT sabadadpour comparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT mohammadmashreghi comparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT javadzarqi comparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT anisaskarizadeh comparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT alibadiee comparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT leilaarabi comparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT seyedehaliamoosavian comparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice AT mahmoudrezajaafari comparisonofbiodistributionofglutathionepegylatednanoliposomaldoxorubicinformulationspreparedbypreinsertionandpostinsertionmethodsforbraindeliveryinnormalmice |