Identification of Iron Homeostasis Genes Dysregulation Potentially Involved in Retinopathy of Prematurity Pathogenicity by Microarray Analysis
Retinopathy of prematurity (ROP) is a serious disease of preterm neonates and there are limited systematic studies of the molecular mechanisms underlying ROP. Therefore, here we performed global gene expression profiling in human fetal retinal microvascular endothelial cells (RMECs) under hypoxic co...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2015/584854 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548090557497344 |
|---|---|
| author | Xian-qiong Luo Chun-yi Zhang Jia-wen Zhang Jing-bo Jiang Ai-hua Yin Li Guo Chuan Nie Xu-zai Lu Hua Deng Liang Zhang |
| author_facet | Xian-qiong Luo Chun-yi Zhang Jia-wen Zhang Jing-bo Jiang Ai-hua Yin Li Guo Chuan Nie Xu-zai Lu Hua Deng Liang Zhang |
| author_sort | Xian-qiong Luo |
| collection | DOAJ |
| description | Retinopathy of prematurity (ROP) is a serious disease of preterm neonates and there are limited systematic studies of the molecular mechanisms underlying ROP. Therefore, here we performed global gene expression profiling in human fetal retinal microvascular endothelial cells (RMECs) under hypoxic conditions in vitro. Aborted fetuses were enrolled and primary RMECs were isolated from eyeballs. Cultivated cells were treated with CoCl2 to induce hypoxia. The dual-color microarray approach was adopted to compare gene expression profiling between treated RMECs and the paired untreated control. The one-class algorithm in significance analysis of microarray (SAM) software was used to screen the differentially expressed genes (DEGs) and quantitative RT-PCR (qRT-PCR) was conducted to validate the results. Gene Ontology was employed for functional enrichment analysis. There were 326 DEGs between the hypoxia-induced group and untreated group. Of these genes, 198 were upregulated in hypoxic RMECs, while the other 128 hits were downregulated. In particular, genes in the iron ion homeostasis pathway were highly enriched under hypoxic conditions. Our study indicates that dysregulation of genes involved in iron homeostasis mediating oxidative damage may be responsible for the mechanisms underlying ROP. The “oxygen plus iron” hypothesis may improve our understanding of ROP pathogenesis. |
| format | Article |
| id | doaj-art-dae13b1e255d437dbbb06cb7aaa9f519 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-dae13b1e255d437dbbb06cb7aaa9f5192025-02-03T06:42:25ZengWileyJournal of Ophthalmology2090-004X2090-00582015-01-01201510.1155/2015/584854584854Identification of Iron Homeostasis Genes Dysregulation Potentially Involved in Retinopathy of Prematurity Pathogenicity by Microarray AnalysisXian-qiong Luo0Chun-yi Zhang1Jia-wen Zhang2Jing-bo Jiang3Ai-hua Yin4Li Guo5Chuan Nie6Xu-zai Lu7Hua Deng8Liang Zhang9Department of Neonatology, Guangdong Women and Children’s Hospital, Guangzhou 511400, ChinaJinan University, Guangzhou 511000, ChinaDepartment of Neonatology, Guangdong Women and Children’s Hospital, Guangzhou 511400, ChinaDepartment of Neonatology, Guangdong Women and Children’s Hospital, Guangzhou 511400, ChinaMedical Genetics Center, Guangdong Women and Children’s Hospital, Guangzhou 511400, ChinaMedical Genetics Center, Guangdong Women and Children’s Hospital, Guangzhou 511400, ChinaDepartment of Neonatology, Guangdong Women and Children’s Hospital, Guangzhou 511400, ChinaDepartment of Neonatology, Guangdong Women and Children’s Hospital, Guangzhou 511400, ChinaTranslational Medicine Center, Guangdong Women and Children’s Hospital, Guangzhou 511400, ChinaTranslational Medicine Center, Guangdong Women and Children’s Hospital, Guangzhou 511400, ChinaRetinopathy of prematurity (ROP) is a serious disease of preterm neonates and there are limited systematic studies of the molecular mechanisms underlying ROP. Therefore, here we performed global gene expression profiling in human fetal retinal microvascular endothelial cells (RMECs) under hypoxic conditions in vitro. Aborted fetuses were enrolled and primary RMECs were isolated from eyeballs. Cultivated cells were treated with CoCl2 to induce hypoxia. The dual-color microarray approach was adopted to compare gene expression profiling between treated RMECs and the paired untreated control. The one-class algorithm in significance analysis of microarray (SAM) software was used to screen the differentially expressed genes (DEGs) and quantitative RT-PCR (qRT-PCR) was conducted to validate the results. Gene Ontology was employed for functional enrichment analysis. There were 326 DEGs between the hypoxia-induced group and untreated group. Of these genes, 198 were upregulated in hypoxic RMECs, while the other 128 hits were downregulated. In particular, genes in the iron ion homeostasis pathway were highly enriched under hypoxic conditions. Our study indicates that dysregulation of genes involved in iron homeostasis mediating oxidative damage may be responsible for the mechanisms underlying ROP. The “oxygen plus iron” hypothesis may improve our understanding of ROP pathogenesis.http://dx.doi.org/10.1155/2015/584854 |
| spellingShingle | Xian-qiong Luo Chun-yi Zhang Jia-wen Zhang Jing-bo Jiang Ai-hua Yin Li Guo Chuan Nie Xu-zai Lu Hua Deng Liang Zhang Identification of Iron Homeostasis Genes Dysregulation Potentially Involved in Retinopathy of Prematurity Pathogenicity by Microarray Analysis Journal of Ophthalmology |
| title | Identification of Iron Homeostasis Genes Dysregulation Potentially Involved in Retinopathy of Prematurity Pathogenicity by Microarray Analysis |
| title_full | Identification of Iron Homeostasis Genes Dysregulation Potentially Involved in Retinopathy of Prematurity Pathogenicity by Microarray Analysis |
| title_fullStr | Identification of Iron Homeostasis Genes Dysregulation Potentially Involved in Retinopathy of Prematurity Pathogenicity by Microarray Analysis |
| title_full_unstemmed | Identification of Iron Homeostasis Genes Dysregulation Potentially Involved in Retinopathy of Prematurity Pathogenicity by Microarray Analysis |
| title_short | Identification of Iron Homeostasis Genes Dysregulation Potentially Involved in Retinopathy of Prematurity Pathogenicity by Microarray Analysis |
| title_sort | identification of iron homeostasis genes dysregulation potentially involved in retinopathy of prematurity pathogenicity by microarray analysis |
| url | http://dx.doi.org/10.1155/2015/584854 |
| work_keys_str_mv | AT xianqiongluo identificationofironhomeostasisgenesdysregulationpotentiallyinvolvedinretinopathyofprematuritypathogenicitybymicroarrayanalysis AT chunyizhang identificationofironhomeostasisgenesdysregulationpotentiallyinvolvedinretinopathyofprematuritypathogenicitybymicroarrayanalysis AT jiawenzhang identificationofironhomeostasisgenesdysregulationpotentiallyinvolvedinretinopathyofprematuritypathogenicitybymicroarrayanalysis AT jingbojiang identificationofironhomeostasisgenesdysregulationpotentiallyinvolvedinretinopathyofprematuritypathogenicitybymicroarrayanalysis AT aihuayin identificationofironhomeostasisgenesdysregulationpotentiallyinvolvedinretinopathyofprematuritypathogenicitybymicroarrayanalysis AT liguo identificationofironhomeostasisgenesdysregulationpotentiallyinvolvedinretinopathyofprematuritypathogenicitybymicroarrayanalysis AT chuannie identificationofironhomeostasisgenesdysregulationpotentiallyinvolvedinretinopathyofprematuritypathogenicitybymicroarrayanalysis AT xuzailu identificationofironhomeostasisgenesdysregulationpotentiallyinvolvedinretinopathyofprematuritypathogenicitybymicroarrayanalysis AT huadeng identificationofironhomeostasisgenesdysregulationpotentiallyinvolvedinretinopathyofprematuritypathogenicitybymicroarrayanalysis AT liangzhang identificationofironhomeostasisgenesdysregulationpotentiallyinvolvedinretinopathyofprematuritypathogenicitybymicroarrayanalysis |