A three-dimensional mouse liver organoid platform for assessing EDCs metabolites simulating liver metabolism
Hepatic metabolism is an important process for evaluate the potential activity and toxicity of endocrine disrupting chemicals (EDCs) metabolites. Organization for Economic Co-operation and Development (OECD) has advocated the development of in vitro assays that mimic in vivo hepatic metabolism to ev...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Environment International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412024007700 |
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| author | Ji Hyun Moon Hyun-Soo Roh Young Jae Park Hyun Ho Song Jieun Choi Da Woon Jung Soo Jin Park Ho Jin Park So-Hyeon Park Da-Eun Kim Gahee Kim Joong-Hyuck Auh Dong Ha Bhang Hong Jin Lee Do Yup Lee |
| author_facet | Ji Hyun Moon Hyun-Soo Roh Young Jae Park Hyun Ho Song Jieun Choi Da Woon Jung Soo Jin Park Ho Jin Park So-Hyeon Park Da-Eun Kim Gahee Kim Joong-Hyuck Auh Dong Ha Bhang Hong Jin Lee Do Yup Lee |
| author_sort | Ji Hyun Moon |
| collection | DOAJ |
| description | Hepatic metabolism is an important process for evaluate the potential activity and toxicity of endocrine disrupting chemicals (EDCs) metabolites. Organization for Economic Co-operation and Development (OECD) has advocated the development of in vitro assays that mimic in vivo hepatic metabolism to eventually replace classical animal tests. In response to this need, we established a 3D mouse liver organoid (mLO) platform that mimics the animal model and is distinct from existing models. We evaluated the effects the activity of EDC metabolites generated through mLOs based on human cell-based reporter gene assays in addition to existing models. This study emphasizes the importance of hepatic ex-vivo and suggests the need a new metabolic model through a 3D mLOs platform. These results indicate that mLOs provides a novel biological method to screen for potential endocrine-disrupting activities of EDC metabolites. |
| format | Article |
| id | doaj-art-dae0faabd17246c7b944d4091d56e289 |
| institution | Kabale University |
| issn | 0160-4120 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj-art-dae0faabd17246c7b944d4091d56e2892025-01-24T04:43:59ZengElsevierEnvironment International0160-41202025-01-01195109184A three-dimensional mouse liver organoid platform for assessing EDCs metabolites simulating liver metabolismJi Hyun Moon0Hyun-Soo Roh1Young Jae Park2Hyun Ho Song3Jieun Choi4Da Woon Jung5Soo Jin Park6Ho Jin Park7So-Hyeon Park8Da-Eun Kim9Gahee Kim10Joong-Hyuck Auh11Dong Ha Bhang12Hong Jin Lee13Do Yup Lee14Department of Agricultural Biotechnology, Seoul National University, Seoul 00826, Republic of KoreaDepartment of Molecular and Cellular Biology, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi 16419, Republic of Korea; Attislab Inc., Anyang, Gyeonggi-Do 14059, Republic of KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul 00826, Republic of KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul 00826, Republic of KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul 00826, Republic of KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul 00826, Republic of KoreaDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USADepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USADepartment of Food Science and Biotechnology, Chung-Ang University, Anseong 17546, South KoreaDepartment of Molecular and Cellular Biology, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi 16419, Republic of KoreaDepartment of Molecular and Cellular Biology, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi 16419, Republic of Korea; Attislab Inc., Anyang, Gyeonggi-Do 14059, Republic of KoreaDepartment of Food Science and Biotechnology, Chung-Ang University, Anseong 17546, South KoreaDepartment of Molecular and Cellular Biology, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi 16419, Republic of Korea; Attislab Inc., Anyang, Gyeonggi-Do 14059, Republic of KoreaDepartment of Food Science and Biotechnology, Chung-Ang University, Anseong 17546, South KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul 00826, Republic of Korea; Department of Food and Animal Biotechnology, Seoul National University, Seoul 00826, Republic of Korea; Center for Food and Bioconvergence, Research Institute for Agricultural and Life Sciences, Interdisciplinary Programs in Agricultural Genomics, Seoul National University, Seoul 00826, Republic of Korea; Green Bio Science & Technology, Bio-Food Industrialization, Seoul National University, 1447 Pyeongchang-daero, Daehwa-myeon, Pyeongchang-gun, Gangwon-do 25354, Republic of Korea; Corresponding author at: College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.Hepatic metabolism is an important process for evaluate the potential activity and toxicity of endocrine disrupting chemicals (EDCs) metabolites. Organization for Economic Co-operation and Development (OECD) has advocated the development of in vitro assays that mimic in vivo hepatic metabolism to eventually replace classical animal tests. In response to this need, we established a 3D mouse liver organoid (mLO) platform that mimics the animal model and is distinct from existing models. We evaluated the effects the activity of EDC metabolites generated through mLOs based on human cell-based reporter gene assays in addition to existing models. This study emphasizes the importance of hepatic ex-vivo and suggests the need a new metabolic model through a 3D mLOs platform. These results indicate that mLOs provides a novel biological method to screen for potential endocrine-disrupting activities of EDC metabolites.http://www.sciencedirect.com/science/article/pii/S0160412024007700Endocrine-disrupting chemicalsOECD test guidelineLiver metabolismMouse liver organoidsBioactivation |
| spellingShingle | Ji Hyun Moon Hyun-Soo Roh Young Jae Park Hyun Ho Song Jieun Choi Da Woon Jung Soo Jin Park Ho Jin Park So-Hyeon Park Da-Eun Kim Gahee Kim Joong-Hyuck Auh Dong Ha Bhang Hong Jin Lee Do Yup Lee A three-dimensional mouse liver organoid platform for assessing EDCs metabolites simulating liver metabolism Environment International Endocrine-disrupting chemicals OECD test guideline Liver metabolism Mouse liver organoids Bioactivation |
| title | A three-dimensional mouse liver organoid platform for assessing EDCs metabolites simulating liver metabolism |
| title_full | A three-dimensional mouse liver organoid platform for assessing EDCs metabolites simulating liver metabolism |
| title_fullStr | A three-dimensional mouse liver organoid platform for assessing EDCs metabolites simulating liver metabolism |
| title_full_unstemmed | A three-dimensional mouse liver organoid platform for assessing EDCs metabolites simulating liver metabolism |
| title_short | A three-dimensional mouse liver organoid platform for assessing EDCs metabolites simulating liver metabolism |
| title_sort | three dimensional mouse liver organoid platform for assessing edcs metabolites simulating liver metabolism |
| topic | Endocrine-disrupting chemicals OECD test guideline Liver metabolism Mouse liver organoids Bioactivation |
| url | http://www.sciencedirect.com/science/article/pii/S0160412024007700 |
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