CUPRAC Voltammetric Determination of Antioxidant Capacity in Tea Samples by Using Screen-Printed Microelectrodes

Measurement of antioxidant capacity represents an analytical major challenge in terms of accuracy, efficiency, rapid response, or low cost of detection methods. Quantification of antioxidant capacity of food samples using disposable screen‐printed microelectrodes (SPMEs) was based on cyclic voltamme...

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Main Authors: Alina Elena Trofin, Lucia Carmen Trincă, Elena Ungureanu, Adina Mirela Ariton
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2019/8012758
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author Alina Elena Trofin
Lucia Carmen Trincă
Elena Ungureanu
Adina Mirela Ariton
author_facet Alina Elena Trofin
Lucia Carmen Trincă
Elena Ungureanu
Adina Mirela Ariton
author_sort Alina Elena Trofin
collection DOAJ
description Measurement of antioxidant capacity represents an analytical major challenge in terms of accuracy, efficiency, rapid response, or low cost of detection methods. Quantification of antioxidant capacity of food samples using disposable screen‐printed microelectrodes (SPMEs) was based on cyclic voltammetry versus open-circuit potential (CV vs OCP) and differential pulse voltammetry (DPV) as compared with spectrophotometric measurement of the CUPRAC reaction with 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox). The SPMEs are organic‐resistant electrodes and thus compatible with food samples and organic solvents used to dissolve trolox. A micropipette was used to release a drop of 50 μL sample on the spotted surface of the SPME sensor/working electrode that was time programmed to function according to the working protocol. The SPME response was linearly correlated with trolox content. This preliminary demonstration was focused on the analysis of tea infusions, due to the simplicity and reproducibility of the samples’ preparations involved. Analytical results of the antioxidant capacity (expressed as mol·L−1 trolox equivalents) of the tea samples showed a good agreement in the case of spectrophotometry and differential pulse voltammetry (R2 > 0.998). DPV with SPME based on CUPRAC reactions was proven to be a promising approach for the characterization of antioxidant capacity of tea samples with rapid response, cost-effectiveness, and simplicity of operation.
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series Journal of Analytical Methods in Chemistry
spelling doaj-art-da930c42c89e484ba800280ba4a3a35c2025-02-03T01:21:57ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732019-01-01201910.1155/2019/80127588012758CUPRAC Voltammetric Determination of Antioxidant Capacity in Tea Samples by Using Screen-Printed MicroelectrodesAlina Elena Trofin0Lucia Carmen Trincă1Elena Ungureanu2Adina Mirela Ariton3Department of Exact Sciences, University of Agricultural Sciences and Veterinary Medicine “Ion Ionescu de la Brad”, Iasi 700490, RomaniaDepartment of Exact Sciences, University of Agricultural Sciences and Veterinary Medicine “Ion Ionescu de la Brad”, Iasi 700490, RomaniaDepartment of Exact Sciences, University of Agricultural Sciences and Veterinary Medicine “Ion Ionescu de la Brad”, Iasi 700490, RomaniaResearch and Development Center for Cattle Breeding Dancu, Iasi 707252, RomaniaMeasurement of antioxidant capacity represents an analytical major challenge in terms of accuracy, efficiency, rapid response, or low cost of detection methods. Quantification of antioxidant capacity of food samples using disposable screen‐printed microelectrodes (SPMEs) was based on cyclic voltammetry versus open-circuit potential (CV vs OCP) and differential pulse voltammetry (DPV) as compared with spectrophotometric measurement of the CUPRAC reaction with 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox). The SPMEs are organic‐resistant electrodes and thus compatible with food samples and organic solvents used to dissolve trolox. A micropipette was used to release a drop of 50 μL sample on the spotted surface of the SPME sensor/working electrode that was time programmed to function according to the working protocol. The SPME response was linearly correlated with trolox content. This preliminary demonstration was focused on the analysis of tea infusions, due to the simplicity and reproducibility of the samples’ preparations involved. Analytical results of the antioxidant capacity (expressed as mol·L−1 trolox equivalents) of the tea samples showed a good agreement in the case of spectrophotometry and differential pulse voltammetry (R2 > 0.998). DPV with SPME based on CUPRAC reactions was proven to be a promising approach for the characterization of antioxidant capacity of tea samples with rapid response, cost-effectiveness, and simplicity of operation.http://dx.doi.org/10.1155/2019/8012758
spellingShingle Alina Elena Trofin
Lucia Carmen Trincă
Elena Ungureanu
Adina Mirela Ariton
CUPRAC Voltammetric Determination of Antioxidant Capacity in Tea Samples by Using Screen-Printed Microelectrodes
Journal of Analytical Methods in Chemistry
title CUPRAC Voltammetric Determination of Antioxidant Capacity in Tea Samples by Using Screen-Printed Microelectrodes
title_full CUPRAC Voltammetric Determination of Antioxidant Capacity in Tea Samples by Using Screen-Printed Microelectrodes
title_fullStr CUPRAC Voltammetric Determination of Antioxidant Capacity in Tea Samples by Using Screen-Printed Microelectrodes
title_full_unstemmed CUPRAC Voltammetric Determination of Antioxidant Capacity in Tea Samples by Using Screen-Printed Microelectrodes
title_short CUPRAC Voltammetric Determination of Antioxidant Capacity in Tea Samples by Using Screen-Printed Microelectrodes
title_sort cuprac voltammetric determination of antioxidant capacity in tea samples by using screen printed microelectrodes
url http://dx.doi.org/10.1155/2019/8012758
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AT elenaungureanu cupracvoltammetricdeterminationofantioxidantcapacityinteasamplesbyusingscreenprintedmicroelectrodes
AT adinamirelaariton cupracvoltammetricdeterminationofantioxidantcapacityinteasamplesbyusingscreenprintedmicroelectrodes