Identification of Sources of Resistance for Peanut Aspergillus flavus Colonization and Aflatoxin Contamination
Peanut aflatoxin contamination caused by Aspergillus flavus is a serious constraint for food safety and human health in Senegal. The present study aimed to identify sources of resistance for A. flavus colonization and aflatoxin contamination. Thus, seeds from 67 peanut genotypes were tested under la...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Agronomy |
| Online Access: | http://dx.doi.org/10.1155/2018/5468602 |
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| author | Richard Moise Alansou Dieme Issa Faye Yedomon Ange Bovys Zoclanclounon Daniel Fonceka Ousmane Ndoye Papa Madiallacke Diedhiou |
| author_facet | Richard Moise Alansou Dieme Issa Faye Yedomon Ange Bovys Zoclanclounon Daniel Fonceka Ousmane Ndoye Papa Madiallacke Diedhiou |
| author_sort | Richard Moise Alansou Dieme |
| collection | DOAJ |
| description | Peanut aflatoxin contamination caused by Aspergillus flavus is a serious constraint for food safety and human health in Senegal. The present study aimed to identify sources of resistance for A. flavus colonization and aflatoxin contamination. Thus, seeds from 67 peanut genotypes were tested under laboratory conditions. Aqueous conidial suspension of an aflatoxinogenic strain of A. flavus was used for inoculation in Petri dishes containing ten seeds of each genotype, and data on incidence and severity were recorded. Total aflatoxin concentration in seeds was determined on 15th day after inoculation using mReader® method. Results showed a significant (p<0.001) variation of aflatoxin, incidence and severity among the tested peanut genotypes. Incidence ranged from 0 to 70% with a mean of 20.36 ± 0.8%. Out of the 67 genotypes, eight showed incidence less than 10%. Severity ranged from 0 to 44% with a mean value of 8.82 ± 0.45%. The genotype 12CS_104 showed aflatoxin concentration level in conformity with the European standard (4 ppb). Out of three clusters revealed by hierarchical classification based on disease incidence and severity, the cluster 1 contained 33 genotypes characterised by low incidence and severity values. These genotypes can be tested under field conditions to confirm their resistance to A flavus. |
| format | Article |
| id | doaj-art-da8f0bdb90fe47d9b0625c4bd4c689c5 |
| institution | Kabale University |
| issn | 1687-8159 1687-8167 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Agronomy |
| spelling | doaj-art-da8f0bdb90fe47d9b0625c4bd4c689c52025-02-03T05:58:25ZengWileyInternational Journal of Agronomy1687-81591687-81672018-01-01201810.1155/2018/54686025468602Identification of Sources of Resistance for Peanut Aspergillus flavus Colonization and Aflatoxin ContaminationRichard Moise Alansou Dieme0Issa Faye1Yedomon Ange Bovys Zoclanclounon2Daniel Fonceka3Ousmane Ndoye4Papa Madiallacke Diedhiou5Université Gaston Berger, BP 211 Saint-Louis, SenegalCentre National de Recherches Agronomiques de Bambey, Institut Sénégalais de Recherches Agricoles, BP 211 Bambey, SenegalCentre d’Etudes Régional pour l’Amélioration de l’Adaptation à la Sécheresse, Route de Khombole, BP 3320 Thiès, SenegalCentre d’Etudes Régional pour l’Amélioration de l’Adaptation à la Sécheresse, Route de Khombole, BP 3320 Thiès, SenegalConseil Ouest et centre Africain pour la Recherche et le Développement Agricoles, Avenue Bourguiba, BP 48 Dakar RP, SenegalUniversité Gaston Berger, BP 211 Saint-Louis, SenegalPeanut aflatoxin contamination caused by Aspergillus flavus is a serious constraint for food safety and human health in Senegal. The present study aimed to identify sources of resistance for A. flavus colonization and aflatoxin contamination. Thus, seeds from 67 peanut genotypes were tested under laboratory conditions. Aqueous conidial suspension of an aflatoxinogenic strain of A. flavus was used for inoculation in Petri dishes containing ten seeds of each genotype, and data on incidence and severity were recorded. Total aflatoxin concentration in seeds was determined on 15th day after inoculation using mReader® method. Results showed a significant (p<0.001) variation of aflatoxin, incidence and severity among the tested peanut genotypes. Incidence ranged from 0 to 70% with a mean of 20.36 ± 0.8%. Out of the 67 genotypes, eight showed incidence less than 10%. Severity ranged from 0 to 44% with a mean value of 8.82 ± 0.45%. The genotype 12CS_104 showed aflatoxin concentration level in conformity with the European standard (4 ppb). Out of three clusters revealed by hierarchical classification based on disease incidence and severity, the cluster 1 contained 33 genotypes characterised by low incidence and severity values. These genotypes can be tested under field conditions to confirm their resistance to A flavus.http://dx.doi.org/10.1155/2018/5468602 |
| spellingShingle | Richard Moise Alansou Dieme Issa Faye Yedomon Ange Bovys Zoclanclounon Daniel Fonceka Ousmane Ndoye Papa Madiallacke Diedhiou Identification of Sources of Resistance for Peanut Aspergillus flavus Colonization and Aflatoxin Contamination International Journal of Agronomy |
| title | Identification of Sources of Resistance for Peanut Aspergillus flavus Colonization and Aflatoxin Contamination |
| title_full | Identification of Sources of Resistance for Peanut Aspergillus flavus Colonization and Aflatoxin Contamination |
| title_fullStr | Identification of Sources of Resistance for Peanut Aspergillus flavus Colonization and Aflatoxin Contamination |
| title_full_unstemmed | Identification of Sources of Resistance for Peanut Aspergillus flavus Colonization and Aflatoxin Contamination |
| title_short | Identification of Sources of Resistance for Peanut Aspergillus flavus Colonization and Aflatoxin Contamination |
| title_sort | identification of sources of resistance for peanut aspergillus flavus colonization and aflatoxin contamination |
| url | http://dx.doi.org/10.1155/2018/5468602 |
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