Phytochemical, Antimicrobial, and Antioxidant Profiles of Duranta erecta L. Parts
The use of plant-based medicine is popular amongst individuals and communities in developing countries. Duranta erecta has been used in Africa and Asia to treat a wide range of diseases. This study evaluated the phytochemical profile and antioxidant and antimicrobial activities of D. erecta to ascer...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2019/8731595 |
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| author | Shadrack Donkor Christopher Larbie Gustav Komlaga Benjamin Obukowho Emikpe |
| author_facet | Shadrack Donkor Christopher Larbie Gustav Komlaga Benjamin Obukowho Emikpe |
| author_sort | Shadrack Donkor |
| collection | DOAJ |
| description | The use of plant-based medicine is popular amongst individuals and communities in developing countries. Duranta erecta has been used in Africa and Asia to treat a wide range of diseases. This study evaluated the phytochemical profile and antioxidant and antimicrobial activities of D. erecta to ascertain its health benefits in traditional medicine. Phytochemical constituents and antimicrobial effect of the hydroethanolic extract of D. erecta leaves (DRL), unripe fruits (DRU), and ripe fruits (DRR) were investigated by standard methods. Elemental analyses were carried out by atomic absorption spectroscopy (AAS) on the raw sample and extract. FTIR and UV-VIS spectroscopy were used to identify functional groups. Extracts were screened for their possible antioxidant activities by three tests. The total phenolic and total tannin contents were evaluated by using the Folin–Ciocalteu method. Total flavonoid content was determined by the aluminium chloride colorimetric assay method. The antioxidant activities were evaluated using the DPPH scavenging activity. The results of phytochemical screening showed the presence of triterpenoids, sterols, alkaloids, flavonoids, saponins, glycosides, and tannins. FTIR analysis revealed the presence of alcohols, phenols, alkanes, aldehydes, ketones, aromatics, aliphatic amines, aromatic amines, amides, carboxylic acids, esters, nitro compounds, alkynes, primary and secondary amines, and alkyl halides. Iron, zinc, and copper were also detected. Total phenolic and tannin contents ranged from 2.20 ± 0.15 to 14.54 ± 0.29 mg gallic acid equivalent (GAE)/100 g and 3.55 ± 0.07 to 13.82 ± 0.04 mg GAE/100 g, respectively. Total flavonoid content varied from 41.76 ± 0.96 to 343.49 ± 3.45 μg quercetin equivalent (QE)/100 g. The highest DPPH scavenging activity was recorded in the methanolic fraction of the leaves. The antimicrobial assay of the extract or fractions recorded no activity against the test organisms. The outcome of this study affirmed that D. erecta contains phytochemicals and bioactive compounds that could be of health benefit. |
| format | Article |
| id | doaj-art-da886da439e0400a9e5bb9e1ccc3b526 |
| institution | OA Journals |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-da886da439e0400a9e5bb9e1ccc3b5262025-08-20T02:21:16ZengWileyBiochemistry Research International2090-22472090-22552019-01-01201910.1155/2019/87315958731595Phytochemical, Antimicrobial, and Antioxidant Profiles of Duranta erecta L. PartsShadrack Donkor0Christopher Larbie1Gustav Komlaga2Benjamin Obukowho Emikpe3Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon, Accra, GhanaDepartment of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, GhanaDepartment of Pharmacognosy, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, GhanaDepartment of Veterinary Pathology, University of Ibadan, Ibadan, NigeriaThe use of plant-based medicine is popular amongst individuals and communities in developing countries. Duranta erecta has been used in Africa and Asia to treat a wide range of diseases. This study evaluated the phytochemical profile and antioxidant and antimicrobial activities of D. erecta to ascertain its health benefits in traditional medicine. Phytochemical constituents and antimicrobial effect of the hydroethanolic extract of D. erecta leaves (DRL), unripe fruits (DRU), and ripe fruits (DRR) were investigated by standard methods. Elemental analyses were carried out by atomic absorption spectroscopy (AAS) on the raw sample and extract. FTIR and UV-VIS spectroscopy were used to identify functional groups. Extracts were screened for their possible antioxidant activities by three tests. The total phenolic and total tannin contents were evaluated by using the Folin–Ciocalteu method. Total flavonoid content was determined by the aluminium chloride colorimetric assay method. The antioxidant activities were evaluated using the DPPH scavenging activity. The results of phytochemical screening showed the presence of triterpenoids, sterols, alkaloids, flavonoids, saponins, glycosides, and tannins. FTIR analysis revealed the presence of alcohols, phenols, alkanes, aldehydes, ketones, aromatics, aliphatic amines, aromatic amines, amides, carboxylic acids, esters, nitro compounds, alkynes, primary and secondary amines, and alkyl halides. Iron, zinc, and copper were also detected. Total phenolic and tannin contents ranged from 2.20 ± 0.15 to 14.54 ± 0.29 mg gallic acid equivalent (GAE)/100 g and 3.55 ± 0.07 to 13.82 ± 0.04 mg GAE/100 g, respectively. Total flavonoid content varied from 41.76 ± 0.96 to 343.49 ± 3.45 μg quercetin equivalent (QE)/100 g. The highest DPPH scavenging activity was recorded in the methanolic fraction of the leaves. The antimicrobial assay of the extract or fractions recorded no activity against the test organisms. The outcome of this study affirmed that D. erecta contains phytochemicals and bioactive compounds that could be of health benefit.http://dx.doi.org/10.1155/2019/8731595 |
| spellingShingle | Shadrack Donkor Christopher Larbie Gustav Komlaga Benjamin Obukowho Emikpe Phytochemical, Antimicrobial, and Antioxidant Profiles of Duranta erecta L. Parts Biochemistry Research International |
| title | Phytochemical, Antimicrobial, and Antioxidant Profiles of Duranta erecta L. Parts |
| title_full | Phytochemical, Antimicrobial, and Antioxidant Profiles of Duranta erecta L. Parts |
| title_fullStr | Phytochemical, Antimicrobial, and Antioxidant Profiles of Duranta erecta L. Parts |
| title_full_unstemmed | Phytochemical, Antimicrobial, and Antioxidant Profiles of Duranta erecta L. Parts |
| title_short | Phytochemical, Antimicrobial, and Antioxidant Profiles of Duranta erecta L. Parts |
| title_sort | phytochemical antimicrobial and antioxidant profiles of duranta erecta l parts |
| url | http://dx.doi.org/10.1155/2019/8731595 |
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