CircRNA-miRNA-mRNA regulatory network in high-altitude hypobaric hypoxia-induced hearing impairment and hearing acclimatization
Objective: High altitude hypobaric hypoxia can induce hearing impairment and hearing acclimatization, but few studies have been performed to decipher the potential transition between the two states. To decipher transition-related circular RNAs (circRNAs)-microRNAs (miRNAs)-messenger RNA (mRNAs) regu...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Brazilian Journal of Otorhinolaryngology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1808869424001721 |
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| author | Danzeng Awang Kanzi Danzeng Tianheng Wang Quzong Deji Mengting Huang Hailong Ren Xinzhu Liu Binghan Zhao Lanzi Gongga |
| author_facet | Danzeng Awang Kanzi Danzeng Tianheng Wang Quzong Deji Mengting Huang Hailong Ren Xinzhu Liu Binghan Zhao Lanzi Gongga |
| author_sort | Danzeng Awang |
| collection | DOAJ |
| description | Objective: High altitude hypobaric hypoxia can induce hearing impairment and hearing acclimatization, but few studies have been performed to decipher the potential transition between the two states. To decipher transition-related circular RNAs (circRNAs)-microRNAs (miRNAs)-messenger RNA (mRNAs) regulatory network. Methods: Wistar rats were airlifted from plain to high altitude and maintained for 30 days and 60 days. Hearing acclimatization was determined using the Auditory Brainstem Response (ABR) test. Cochlea tissues were isolated, and high-throughput circRNA analysis and mRNAs-sequencing were performed. Differentially Expressed circRNAs (DEcircRNAs) and Differentially Expressed mRNAs (DEmRNAs) were obtained, and circRNA-miRNA and miRNA-mRNA regulation were predicted. A circRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network was also constructed. The DEmRNAs in this network were functionally annotated using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses based on Metascape. Results: The ABR assay indicated that hearing impairment happened on day 30 and hearing acclimatization occurred on day 60. Hearing impairment-related circRNAs (64 upregulated and 147 downregulated) and genes (572 upregulated and 757 downregulated) were identified. Hearing acclimatization-related circRNAs (79 upregulated and 142 downregulated) and genes (690 upregulated and 751 downregulated) were also identified. Hearing impairment and hearing acclimatization ceRNA networks were also constructed after integrating the predicted miRNA regulation analyses. Anterograde trans-synaptic signaling (GO:0098916) and negative regulation of cellular response to growth factor stimulus (GO:0090288) were regulated by hearing impairment ceRNA networks, and embryonic organ development (GO:0048568) was regulated by hearing acclimatization ceRNA networks. Conclusion: Hearing impairment- and hearing acclimatization-associated circRNAs and ceRNA networks were identified, which contribute new knowledge to our understanding of acclimatization transition. |
| format | Article |
| id | doaj-art-da7d2dd29eec44979a0b56a9f2edaf6c |
| institution | Kabale University |
| issn | 1808-8694 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Otorhinolaryngology |
| spelling | doaj-art-da7d2dd29eec44979a0b56a9f2edaf6c2025-01-29T05:00:26ZengElsevierBrazilian Journal of Otorhinolaryngology1808-86942025-03-01912101557CircRNA-miRNA-mRNA regulatory network in high-altitude hypobaric hypoxia-induced hearing impairment and hearing acclimatizationDanzeng Awang0Kanzi Danzeng1Tianheng Wang2Quzong Deji3Mengting Huang4Hailong Ren5Xinzhu Liu6Binghan Zhao7Lanzi Gongga8Medical College, Tibet University, Department of Clinical Medicine, Lhasa, China; Tibet University, Medical College, Lhasa, ChinaLhasa People's Hospital, Children's Surgery Department, Lhasa, ChinaHealth Service Center of Jiri Street Office, Chengguan District, Lhasa, ChinaTibet University, Medical College, Lhasa, ChinaHealth Service Center of Jiri Street Office, Chengguan District, Lhasa, ChinaTibet University, Medical College, Lhasa, ChinaTibet University, Medical College, Lhasa, ChinaTibet University, Medical College, Lhasa, ChinaTibet University, Medical College, Lhasa, China; Corresponding author.Objective: High altitude hypobaric hypoxia can induce hearing impairment and hearing acclimatization, but few studies have been performed to decipher the potential transition between the two states. To decipher transition-related circular RNAs (circRNAs)-microRNAs (miRNAs)-messenger RNA (mRNAs) regulatory network. Methods: Wistar rats were airlifted from plain to high altitude and maintained for 30 days and 60 days. Hearing acclimatization was determined using the Auditory Brainstem Response (ABR) test. Cochlea tissues were isolated, and high-throughput circRNA analysis and mRNAs-sequencing were performed. Differentially Expressed circRNAs (DEcircRNAs) and Differentially Expressed mRNAs (DEmRNAs) were obtained, and circRNA-miRNA and miRNA-mRNA regulation were predicted. A circRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network was also constructed. The DEmRNAs in this network were functionally annotated using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses based on Metascape. Results: The ABR assay indicated that hearing impairment happened on day 30 and hearing acclimatization occurred on day 60. Hearing impairment-related circRNAs (64 upregulated and 147 downregulated) and genes (572 upregulated and 757 downregulated) were identified. Hearing acclimatization-related circRNAs (79 upregulated and 142 downregulated) and genes (690 upregulated and 751 downregulated) were also identified. Hearing impairment and hearing acclimatization ceRNA networks were also constructed after integrating the predicted miRNA regulation analyses. Anterograde trans-synaptic signaling (GO:0098916) and negative regulation of cellular response to growth factor stimulus (GO:0090288) were regulated by hearing impairment ceRNA networks, and embryonic organ development (GO:0048568) was regulated by hearing acclimatization ceRNA networks. Conclusion: Hearing impairment- and hearing acclimatization-associated circRNAs and ceRNA networks were identified, which contribute new knowledge to our understanding of acclimatization transition.http://www.sciencedirect.com/science/article/pii/S1808869424001721circRNAsHigh altitudeHypobaric hypoxiaHearing acclimatizationHearing impairment |
| spellingShingle | Danzeng Awang Kanzi Danzeng Tianheng Wang Quzong Deji Mengting Huang Hailong Ren Xinzhu Liu Binghan Zhao Lanzi Gongga CircRNA-miRNA-mRNA regulatory network in high-altitude hypobaric hypoxia-induced hearing impairment and hearing acclimatization Brazilian Journal of Otorhinolaryngology circRNAs High altitude Hypobaric hypoxia Hearing acclimatization Hearing impairment |
| title | CircRNA-miRNA-mRNA regulatory network in high-altitude hypobaric hypoxia-induced hearing impairment and hearing acclimatization |
| title_full | CircRNA-miRNA-mRNA regulatory network in high-altitude hypobaric hypoxia-induced hearing impairment and hearing acclimatization |
| title_fullStr | CircRNA-miRNA-mRNA regulatory network in high-altitude hypobaric hypoxia-induced hearing impairment and hearing acclimatization |
| title_full_unstemmed | CircRNA-miRNA-mRNA regulatory network in high-altitude hypobaric hypoxia-induced hearing impairment and hearing acclimatization |
| title_short | CircRNA-miRNA-mRNA regulatory network in high-altitude hypobaric hypoxia-induced hearing impairment and hearing acclimatization |
| title_sort | circrna mirna mrna regulatory network in high altitude hypobaric hypoxia induced hearing impairment and hearing acclimatization |
| topic | circRNAs High altitude Hypobaric hypoxia Hearing acclimatization Hearing impairment |
| url | http://www.sciencedirect.com/science/article/pii/S1808869424001721 |
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