Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in Dogs
The use of stem cells in injury repair has been extensively investigated. Here, we examined the therapeutic effects of autologous bone marrow mesenchymal stem cells (MSC) transplantation in four dogs with natural traumatic spinal cord injuries. MSC were cultured in vitro, and proliferation rate and...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2014/437521 |
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| author | Euler Moraes Penha Cássio Santana Meira Elisalva Teixeira Guimarães Marcus Vinícius Pinheiro Mendonça Faye Alice Gravely Cláudia Maria Bahia Pinheiro Taiana Maria Bahia Pinheiro Stella Maria Barrouin-Melo Ricardo Ribeiro-dos-Santos Milena Botelho Pereira Soares |
| author_facet | Euler Moraes Penha Cássio Santana Meira Elisalva Teixeira Guimarães Marcus Vinícius Pinheiro Mendonça Faye Alice Gravely Cláudia Maria Bahia Pinheiro Taiana Maria Bahia Pinheiro Stella Maria Barrouin-Melo Ricardo Ribeiro-dos-Santos Milena Botelho Pereira Soares |
| author_sort | Euler Moraes Penha |
| collection | DOAJ |
| description | The use of stem cells in injury repair has been extensively investigated. Here, we examined the therapeutic effects of autologous bone marrow mesenchymal stem cells (MSC) transplantation in four dogs with natural traumatic spinal cord injuries. MSC were cultured in vitro, and proliferation rate and cell viability were evaluated. Cell suspensions were prepared and surgically administered into the spinal cord. The animals were clinically evaluated and examined by nuclear magnetic resonance. Ten days after the surgical procedure and MSC transplantation, we observed a progressive recovery of the panniculus reflex and diminished superficial and deep pain response, although there were still low proprioceptive reflexes in addition to a hyperreflex in the ataxic hind limb movement responses. Each dog demonstrated an improvement in these gains over time. Conscious reflex recovery occurred simultaneously with moderate improvement in intestine and urinary bladder functions in two of the four dogs. By the 18th month of clinical monitoring, we observed a remarkable clinical amelioration accompanied by improved movement, in three of the four dogs. However, no clinical gain was associated with alterations in magnetic resonance imaging. Our results indicate that MSC are potential candidates for the stem cell therapy following spinal cord injury. |
| format | Article |
| id | doaj-art-da6a6e141ec34061bc482d0f43982da8 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-da6a6e141ec34061bc482d0f43982da82025-02-03T05:51:26ZengWileyStem Cells International1687-966X1687-96782014-01-01201410.1155/2014/437521437521Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in DogsEuler Moraes Penha0Cássio Santana Meira1Elisalva Teixeira Guimarães2Marcus Vinícius Pinheiro Mendonça3Faye Alice Gravely4Cláudia Maria Bahia Pinheiro5Taiana Maria Bahia Pinheiro6Stella Maria Barrouin-Melo7Ricardo Ribeiro-dos-Santos8Milena Botelho Pereira Soares9Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, 40296-710 Salvador, BA, BrazilCentro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, 40296-710 Salvador, BA, BrazilCentro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, 40296-710 Salvador, BA, BrazilHospital Espanhol, 40140-110 Salvador, BA, BrazilA Arca Veterinária, 40243-045 Salvador, BA, BrazilEstácio-FIB, Centro Universitário Estácio da Bahia, 41770-030 Salvador, BA, BrazilEstácio-FIB, Centro Universitário Estácio da Bahia, 41770-030 Salvador, BA, BrazilHospital de Medicina Veterinária, Escola de Medicina Veterinária e Zootecnia, Universidade Federal da Bahia, 40170-110 Salvador, BA, BrazilCentro de Biotecnologia e Terapia Celular, Hospital São Rafael, 41253-190 Salvador, BA, BrazilCentro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, 40296-710 Salvador, BA, BrazilThe use of stem cells in injury repair has been extensively investigated. Here, we examined the therapeutic effects of autologous bone marrow mesenchymal stem cells (MSC) transplantation in four dogs with natural traumatic spinal cord injuries. MSC were cultured in vitro, and proliferation rate and cell viability were evaluated. Cell suspensions were prepared and surgically administered into the spinal cord. The animals were clinically evaluated and examined by nuclear magnetic resonance. Ten days after the surgical procedure and MSC transplantation, we observed a progressive recovery of the panniculus reflex and diminished superficial and deep pain response, although there were still low proprioceptive reflexes in addition to a hyperreflex in the ataxic hind limb movement responses. Each dog demonstrated an improvement in these gains over time. Conscious reflex recovery occurred simultaneously with moderate improvement in intestine and urinary bladder functions in two of the four dogs. By the 18th month of clinical monitoring, we observed a remarkable clinical amelioration accompanied by improved movement, in three of the four dogs. However, no clinical gain was associated with alterations in magnetic resonance imaging. Our results indicate that MSC are potential candidates for the stem cell therapy following spinal cord injury.http://dx.doi.org/10.1155/2014/437521 |
| spellingShingle | Euler Moraes Penha Cássio Santana Meira Elisalva Teixeira Guimarães Marcus Vinícius Pinheiro Mendonça Faye Alice Gravely Cláudia Maria Bahia Pinheiro Taiana Maria Bahia Pinheiro Stella Maria Barrouin-Melo Ricardo Ribeiro-dos-Santos Milena Botelho Pereira Soares Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in Dogs Stem Cells International |
| title | Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in Dogs |
| title_full | Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in Dogs |
| title_fullStr | Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in Dogs |
| title_full_unstemmed | Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in Dogs |
| title_short | Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in Dogs |
| title_sort | use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs |
| url | http://dx.doi.org/10.1155/2014/437521 |
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