Valorizing corn stover waste into valuable bioproducts using subcritical water hydrolysis

This study examined the behavior of the semi-continuous hydrolysis process of corn stover (CS) in subcritical water, focusing on the effects of varying pH levels (1, 2, 4.5, 7, and 8) and temperatures (113, 130, 170, 210, and 226°C). The results showed that the process at 170°C and pH 1 was able to...

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Main Authors: Rafael Gabriel da Rosa, Luiz Eduardo Nochi Castro, Tiago Linhares Cruz Tabosa Barroso, Vanessa Cosme Ferreira, Paulo Rodrigo Stival Bittencourt, Maurício Ariel Rostagno, Tânia Forster-Carneiro
Format: Article
Language:English
Published: Alpha Creation Enterprise 2025-03-01
Series:Biofuel Research Journal
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Online Access:https://www.biofueljournal.com/article_216413_d4ec03842906d0b9cebb41517753a427.pdf
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author Rafael Gabriel da Rosa
Luiz Eduardo Nochi Castro
Tiago Linhares Cruz Tabosa Barroso
Vanessa Cosme Ferreira
Paulo Rodrigo Stival Bittencourt
Maurício Ariel Rostagno
Tânia Forster-Carneiro
author_facet Rafael Gabriel da Rosa
Luiz Eduardo Nochi Castro
Tiago Linhares Cruz Tabosa Barroso
Vanessa Cosme Ferreira
Paulo Rodrigo Stival Bittencourt
Maurício Ariel Rostagno
Tânia Forster-Carneiro
author_sort Rafael Gabriel da Rosa
collection DOAJ
description This study examined the behavior of the semi-continuous hydrolysis process of corn stover (CS) in subcritical water, focusing on the effects of varying pH levels (1, 2, 4.5, 7, and 8) and temperatures (113, 130, 170, 210, and 226°C). The results showed that the process at 170°C and pH 1 was able to recover the highest amount of phenolic compounds (76.82 mg Gallic Acid Equivalents g⁻¹), consequently demonstrating the highest antioxidant activities by the Ferric Reducing Antioxidant Power (FRAP) (423.85 μMol TEAC g⁻¹) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (293.12 μMol TEAC g⁻¹) methods. Additionally, it was possible to obtain the highest concentration of sugars (cellobiose, glucose, and xylose) (460.92 mg g⁻¹). High temperatures (226°C and pH 4.5) produced the highest amount of organic acids (1,157.19 mg g⁻¹). The formation of inhibitors was observed only at temperatures of 170 and 210 °C at a pH of 1.0. The highest yields of sugars, organic acids, and inhibitors were 0.565, 1.123, and 0.665 mg g⁻¹ of carbohydrates, respectively. The statistical analysis identified the optimal conditions for the recovery of various compounds: phenolic compounds at 190.7 °C and pH 1, soluble proteins at 187.4 °C and pH 1, sugars at 134.9 °C and pH 1, organic acids at 223.59 °C and pH 4.1, and for minimizing inhibitors at 114.95 °C and pH 7.3. EcoScale analysis identified subcritical water as the most sustainable and efficient method for CS hydrolysis. Subcritical water treatment effectively recovers valuable compounds from CS, promoting a circular economy by valorizing waste and reducing resource dependence.
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publishDate 2025-03-01
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spelling doaj-art-da3ffb5fe8b841f7bf1bc4e2f749941f2025-08-20T02:10:39ZengAlpha Creation EnterpriseBiofuel Research Journal2292-87822025-03-011212283230510.18331/BRJ2025.12.1.2216413Valorizing corn stover waste into valuable bioproducts using subcritical water hydrolysisRafael Gabriel da Rosa0Luiz Eduardo Nochi Castro1Tiago Linhares Cruz Tabosa Barroso2Vanessa Cosme Ferreira3Paulo Rodrigo Stival Bittencourt4Maurício Ariel Rostagno5Tânia Forster-Carneiro6System School of Food Engineering (FEA), University of Campinas (UNICAMP), Rua Monteiro Lobato, Campinas, São Paulo, Brazil.System School of Food Engineering (FEA), University of Campinas (UNICAMP), Rua Monteiro Lobato, Campinas, São Paulo, Brazil.System School of Food Engineering (FEA), University of Campinas (UNICAMP), Rua Monteiro Lobato, Campinas, São Paulo, Brazil.System School of Food Engineering (FEA), University of Campinas (UNICAMP), Rua Monteiro Lobato, Campinas, São Paulo, Brazil.Department of Chemistry, Federal Technological University of Parana (UTFPR), Medianeira, Paraná, Brazil.School of Applied Sciences (FCA), University of Campinas (UNICAMP), Limeira, São Paulo, Brazil.System School of Food Engineering (FEA), University of Campinas (UNICAMP), Rua Monteiro Lobato, Campinas, São Paulo, Brazil.This study examined the behavior of the semi-continuous hydrolysis process of corn stover (CS) in subcritical water, focusing on the effects of varying pH levels (1, 2, 4.5, 7, and 8) and temperatures (113, 130, 170, 210, and 226°C). The results showed that the process at 170°C and pH 1 was able to recover the highest amount of phenolic compounds (76.82 mg Gallic Acid Equivalents g⁻¹), consequently demonstrating the highest antioxidant activities by the Ferric Reducing Antioxidant Power (FRAP) (423.85 μMol TEAC g⁻¹) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (293.12 μMol TEAC g⁻¹) methods. Additionally, it was possible to obtain the highest concentration of sugars (cellobiose, glucose, and xylose) (460.92 mg g⁻¹). High temperatures (226°C and pH 4.5) produced the highest amount of organic acids (1,157.19 mg g⁻¹). The formation of inhibitors was observed only at temperatures of 170 and 210 °C at a pH of 1.0. The highest yields of sugars, organic acids, and inhibitors were 0.565, 1.123, and 0.665 mg g⁻¹ of carbohydrates, respectively. The statistical analysis identified the optimal conditions for the recovery of various compounds: phenolic compounds at 190.7 °C and pH 1, soluble proteins at 187.4 °C and pH 1, sugars at 134.9 °C and pH 1, organic acids at 223.59 °C and pH 4.1, and for minimizing inhibitors at 114.95 °C and pH 7.3. EcoScale analysis identified subcritical water as the most sustainable and efficient method for CS hydrolysis. Subcritical water treatment effectively recovers valuable compounds from CS, promoting a circular economy by valorizing waste and reducing resource dependence.https://www.biofueljournal.com/article_216413_d4ec03842906d0b9cebb41517753a427.pdfsustainable technologybiorefineryrenewable resourcesphenolic compoundsfermentable sugarscorn biomass
spellingShingle Rafael Gabriel da Rosa
Luiz Eduardo Nochi Castro
Tiago Linhares Cruz Tabosa Barroso
Vanessa Cosme Ferreira
Paulo Rodrigo Stival Bittencourt
Maurício Ariel Rostagno
Tânia Forster-Carneiro
Valorizing corn stover waste into valuable bioproducts using subcritical water hydrolysis
Biofuel Research Journal
sustainable technology
biorefinery
renewable resources
phenolic compounds
fermentable sugars
corn biomass
title Valorizing corn stover waste into valuable bioproducts using subcritical water hydrolysis
title_full Valorizing corn stover waste into valuable bioproducts using subcritical water hydrolysis
title_fullStr Valorizing corn stover waste into valuable bioproducts using subcritical water hydrolysis
title_full_unstemmed Valorizing corn stover waste into valuable bioproducts using subcritical water hydrolysis
title_short Valorizing corn stover waste into valuable bioproducts using subcritical water hydrolysis
title_sort valorizing corn stover waste into valuable bioproducts using subcritical water hydrolysis
topic sustainable technology
biorefinery
renewable resources
phenolic compounds
fermentable sugars
corn biomass
url https://www.biofueljournal.com/article_216413_d4ec03842906d0b9cebb41517753a427.pdf
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