The first insight into Acanthocephalus (Palaeacanthocephala) satellitome: species-specific satellites as potential cytogenetic markers
Abstract Acanthocephalan parasites are often overlooked in many areas of research, and satellitome and cytogenetic analyzes are no exception. The species of the genus Acanthocephalus are known for their very small chromosomes with ambiguous morphology, which makes karyotyping difficult. In this stud...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-85728-2 |
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| author | Anna Marková Martina Orosová Pablo Mora Michal Benovics Pedro Lorite |
| author_facet | Anna Marková Martina Orosová Pablo Mora Michal Benovics Pedro Lorite |
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| description | Abstract Acanthocephalan parasites are often overlooked in many areas of research, and satellitome and cytogenetic analyzes are no exception. The species of the genus Acanthocephalus are known for their very small chromosomes with ambiguous morphology, which makes karyotyping difficult. In this study, we performed the first satellitome analysis of three Acanthocephalus species to identify species- and chromosome-specific satellites that could serve as cytogenetic markers. RepeatExplorer2 revealed a remarkably high number of species-specific repeats, with a predominance of satellite DNAs, alongside variations in repetitive content between sexes. Five satellites in A. anguillae, two in A. lucii and six in A. ranae were successfully mapped to chromosomes using FISH. Each satellite showed a clustered hybridization signal at specific chromosomal locations, which allowed us to create a schematic representation of the distribution of satellites for each species. These newly identified satellites proved to be useful chromosomal markers for the accurate identification of homologous chromosome pairs. No FISH-positive signals were observed on the supernumerary chromosomes of A. anguillae and A. lucii, supporting the hypothesis that these chromosomes have recent origin. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-da3e3b9f87fe42d8a3106d05b4f30bc72025-01-26T12:25:55ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-025-85728-2The first insight into Acanthocephalus (Palaeacanthocephala) satellitome: species-specific satellites as potential cytogenetic markersAnna Marková0Martina Orosová1Pablo Mora2Michal Benovics3Pedro Lorite4Institute of Parasitology, Slovak Academy of SciencesInstitute of Parasitology, Slovak Academy of SciencesDepartment of Experimental Biology, Genetics Area, University of JaénDepartment of Zoology, Faculty of Natural Sciences, Comenius UniversityDepartment of Experimental Biology, Genetics Area, University of JaénAbstract Acanthocephalan parasites are often overlooked in many areas of research, and satellitome and cytogenetic analyzes are no exception. The species of the genus Acanthocephalus are known for their very small chromosomes with ambiguous morphology, which makes karyotyping difficult. In this study, we performed the first satellitome analysis of three Acanthocephalus species to identify species- and chromosome-specific satellites that could serve as cytogenetic markers. RepeatExplorer2 revealed a remarkably high number of species-specific repeats, with a predominance of satellite DNAs, alongside variations in repetitive content between sexes. Five satellites in A. anguillae, two in A. lucii and six in A. ranae were successfully mapped to chromosomes using FISH. Each satellite showed a clustered hybridization signal at specific chromosomal locations, which allowed us to create a schematic representation of the distribution of satellites for each species. These newly identified satellites proved to be useful chromosomal markers for the accurate identification of homologous chromosome pairs. No FISH-positive signals were observed on the supernumerary chromosomes of A. anguillae and A. lucii, supporting the hypothesis that these chromosomes have recent origin.https://doi.org/10.1038/s41598-025-85728-2AcanthocephalaSatellite DNARepeatRepeatExplorer2Fluorescence in situ hybridization |
| spellingShingle | Anna Marková Martina Orosová Pablo Mora Michal Benovics Pedro Lorite The first insight into Acanthocephalus (Palaeacanthocephala) satellitome: species-specific satellites as potential cytogenetic markers Scientific Reports Acanthocephala Satellite DNA Repeat RepeatExplorer2 Fluorescence in situ hybridization |
| title | The first insight into Acanthocephalus (Palaeacanthocephala) satellitome: species-specific satellites as potential cytogenetic markers |
| title_full | The first insight into Acanthocephalus (Palaeacanthocephala) satellitome: species-specific satellites as potential cytogenetic markers |
| title_fullStr | The first insight into Acanthocephalus (Palaeacanthocephala) satellitome: species-specific satellites as potential cytogenetic markers |
| title_full_unstemmed | The first insight into Acanthocephalus (Palaeacanthocephala) satellitome: species-specific satellites as potential cytogenetic markers |
| title_short | The first insight into Acanthocephalus (Palaeacanthocephala) satellitome: species-specific satellites as potential cytogenetic markers |
| title_sort | first insight into acanthocephalus palaeacanthocephala satellitome species specific satellites as potential cytogenetic markers |
| topic | Acanthocephala Satellite DNA Repeat RepeatExplorer2 Fluorescence in situ hybridization |
| url | https://doi.org/10.1038/s41598-025-85728-2 |
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