The transcriptome of MHV‐infected RAW264.7 cells offers an alternative model for macrophage innate immunity research
Abstract Background Macrophages are the primary innate immune cells encountered by the invading coronaviruses, and their abilities to initiate inflammatory reactions, to maintain the immunity homeostasis by differential polarization, to train the innate immune system by epigenic modification have be...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
|
| Series: | Animal Models and Experimental Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ame2.12443 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832087843424436224 |
|---|---|
| author | Yun Liu Ting‐Ting Feng Wei Tong Zhi Guo Xia Li Qi Kong Zhi‐Guang Xiang |
| author_facet | Yun Liu Ting‐Ting Feng Wei Tong Zhi Guo Xia Li Qi Kong Zhi‐Guang Xiang |
| author_sort | Yun Liu |
| collection | DOAJ |
| description | Abstract Background Macrophages are the primary innate immune cells encountered by the invading coronaviruses, and their abilities to initiate inflammatory reactions, to maintain the immunity homeostasis by differential polarization, to train the innate immune system by epigenic modification have been reported in laboratory animal research. Methods In the current in vitro research, murine macrophage RAW 264.7 cell were infected by mouse hepatitis virus, a coronavirus existed in mouse. At 3‐, 6‐, 12‐, 24‐, and 48‐h post infection (hpi.), the attached cells were washed with PBS and harvested in Trizol reagent. Then The harvest is subjected to transcriptome sequencing. Results The transcriptome analysis showed the immediate (3 hpi.) up regulation of DEGs related to inflammation, like Il1b and Il6. DEGs related to M2 differential polarization, like Irf4 showed up regulation at 24 hpi., the late term after viral infection. In addition, DEGs related to metabolism and histone modification, like Ezh2 were detected, which might correlate with the trained immunity of macrophages. Conclusions The current in vitro viral infection study showed the key innated immunity character of macrophages, which suggested the replacement value of viral infection cells model, to reduce the animal usage in preclinical research. |
| format | Article |
| id | doaj-art-da1bf80e15f44258849c9a79bef2dcb2 |
| institution | Kabale University |
| issn | 2576-2095 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Animal Models and Experimental Medicine |
| spelling | doaj-art-da1bf80e15f44258849c9a79bef2dcb22025-02-06T03:52:55ZengWileyAnimal Models and Experimental Medicine2576-20952025-01-0181576610.1002/ame2.12443The transcriptome of MHV‐infected RAW264.7 cells offers an alternative model for macrophage innate immunity researchYun Liu0Ting‐Ting Feng1Wei Tong2Zhi Guo3Xia Li4Qi Kong5Zhi‐Guang Xiang6Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Comparative Medicine Center Peking Union Medical College (PUMC) Beijing P.R. ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Comparative Medicine Center Peking Union Medical College (PUMC) Beijing P.R. ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Comparative Medicine Center Peking Union Medical College (PUMC) Beijing P.R. ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Comparative Medicine Center Peking Union Medical College (PUMC) Beijing P.R. ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Comparative Medicine Center Peking Union Medical College (PUMC) Beijing P.R. ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Comparative Medicine Center Peking Union Medical College (PUMC) Beijing P.R. ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Comparative Medicine Center Peking Union Medical College (PUMC) Beijing P.R. ChinaAbstract Background Macrophages are the primary innate immune cells encountered by the invading coronaviruses, and their abilities to initiate inflammatory reactions, to maintain the immunity homeostasis by differential polarization, to train the innate immune system by epigenic modification have been reported in laboratory animal research. Methods In the current in vitro research, murine macrophage RAW 264.7 cell were infected by mouse hepatitis virus, a coronavirus existed in mouse. At 3‐, 6‐, 12‐, 24‐, and 48‐h post infection (hpi.), the attached cells were washed with PBS and harvested in Trizol reagent. Then The harvest is subjected to transcriptome sequencing. Results The transcriptome analysis showed the immediate (3 hpi.) up regulation of DEGs related to inflammation, like Il1b and Il6. DEGs related to M2 differential polarization, like Irf4 showed up regulation at 24 hpi., the late term after viral infection. In addition, DEGs related to metabolism and histone modification, like Ezh2 were detected, which might correlate with the trained immunity of macrophages. Conclusions The current in vitro viral infection study showed the key innated immunity character of macrophages, which suggested the replacement value of viral infection cells model, to reduce the animal usage in preclinical research.https://doi.org/10.1002/ame2.12443coronavirusinnate immunitymacrophagetranscriptome |
| spellingShingle | Yun Liu Ting‐Ting Feng Wei Tong Zhi Guo Xia Li Qi Kong Zhi‐Guang Xiang The transcriptome of MHV‐infected RAW264.7 cells offers an alternative model for macrophage innate immunity research Animal Models and Experimental Medicine coronavirus innate immunity macrophage transcriptome |
| title | The transcriptome of MHV‐infected RAW264.7 cells offers an alternative model for macrophage innate immunity research |
| title_full | The transcriptome of MHV‐infected RAW264.7 cells offers an alternative model for macrophage innate immunity research |
| title_fullStr | The transcriptome of MHV‐infected RAW264.7 cells offers an alternative model for macrophage innate immunity research |
| title_full_unstemmed | The transcriptome of MHV‐infected RAW264.7 cells offers an alternative model for macrophage innate immunity research |
| title_short | The transcriptome of MHV‐infected RAW264.7 cells offers an alternative model for macrophage innate immunity research |
| title_sort | transcriptome of mhv infected raw264 7 cells offers an alternative model for macrophage innate immunity research |
| topic | coronavirus innate immunity macrophage transcriptome |
| url | https://doi.org/10.1002/ame2.12443 |
| work_keys_str_mv | AT yunliu thetranscriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch AT tingtingfeng thetranscriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch AT weitong thetranscriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch AT zhiguo thetranscriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch AT xiali thetranscriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch AT qikong thetranscriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch AT zhiguangxiang thetranscriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch AT yunliu transcriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch AT tingtingfeng transcriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch AT weitong transcriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch AT zhiguo transcriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch AT xiali transcriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch AT qikong transcriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch AT zhiguangxiang transcriptomeofmhvinfectedraw2647cellsoffersanalternativemodelformacrophageinnateimmunityresearch |