Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil
This study aimed to isolate, purify, and characterize a potential thermophilic cellulase-producing bacterium from the Himalayan soil. Eleven thermophilic bacteria were isolated, and the strain PANG L was found to be the most potent cellulolytic producer. Morphological, physiological, biochemical, an...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2023/3615757 |
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| author | Manita Shyaula Sunil Regmi Deegendra Khadka Ram Chandra Poudel Agni Dhakal Devesh Koirala Jaishree Sijapati Anjana Singh Jyoti Maharjan |
| author_facet | Manita Shyaula Sunil Regmi Deegendra Khadka Ram Chandra Poudel Agni Dhakal Devesh Koirala Jaishree Sijapati Anjana Singh Jyoti Maharjan |
| author_sort | Manita Shyaula |
| collection | DOAJ |
| description | This study aimed to isolate, purify, and characterize a potential thermophilic cellulase-producing bacterium from the Himalayan soil. Eleven thermophilic bacteria were isolated, and the strain PANG L was found to be the most potent cellulolytic producer. Morphological, physiological, biochemical, and molecular characterization identified PANG L as Bacillus licheniformis. This is the first study on the isolation of thermostable cellulase-producing Bacillus licheniformis from the Himalayan soil. This bacterium was processed for the production of cellulase enzyme. The optimum conditions for cellulase production were achieved at 45°C after 48 h of incubation at pH 6.5 in media-containing carboxymethyl cellulose (CMC) and yeast extract as carbon and nitrogen sources, respectively, in a thermo-shaker at 100 rpm. The enzyme was partially purified by 80% ammonium sulphate precipitation followed by dialysis, resulting in a 1.52-fold purification. The optimal activity of partially purified cellulase was observed at a temperature of 60°C and pH 5. The cellulase enzyme was stable within the pH ranges of 3–5 and retained 67% of activity even at 55°C. Cellulase activity was found to be enhanced in the presence of metal ions such as Cd2+, Pb2+, and Ba2+. The enzyme showed the highest activity when CMC was used as a substrate, followed by cellobiose. The Km and Vmax values of the enzyme were 1.8 mg/ml and 10.92 μg/ml/min, respectively. The cellulase enzyme obtained from Bacillus licheniformis PANG L had suitable catalytic properties for use in industrial applications. |
| format | Article |
| id | doaj-art-da1b7da2aa1e4bbebca8b5e6aca4b9da |
| institution | Kabale University |
| issn | 1687-9198 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-da1b7da2aa1e4bbebca8b5e6aca4b9da2025-08-20T03:24:22ZengWileyInternational Journal of Microbiology1687-91982023-01-01202310.1155/2023/3615757Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan SoilManita Shyaula0Sunil Regmi1Deegendra Khadka2Ram Chandra Poudel3Agni Dhakal4Devesh Koirala5Jaishree Sijapati6Anjana Singh7Jyoti Maharjan8Central Department of MicrobiologyNepal Academy of Science and TechnologyNepal Academy of Science and TechnologyNepal Academy of Science and TechnologyNepal Academy of Science and TechnologyNepal Academy of Science and TechnologyNepal Academy of Science and TechnologyCentral Department of MicrobiologyNepal Academy of Science and TechnologyThis study aimed to isolate, purify, and characterize a potential thermophilic cellulase-producing bacterium from the Himalayan soil. Eleven thermophilic bacteria were isolated, and the strain PANG L was found to be the most potent cellulolytic producer. Morphological, physiological, biochemical, and molecular characterization identified PANG L as Bacillus licheniformis. This is the first study on the isolation of thermostable cellulase-producing Bacillus licheniformis from the Himalayan soil. This bacterium was processed for the production of cellulase enzyme. The optimum conditions for cellulase production were achieved at 45°C after 48 h of incubation at pH 6.5 in media-containing carboxymethyl cellulose (CMC) and yeast extract as carbon and nitrogen sources, respectively, in a thermo-shaker at 100 rpm. The enzyme was partially purified by 80% ammonium sulphate precipitation followed by dialysis, resulting in a 1.52-fold purification. The optimal activity of partially purified cellulase was observed at a temperature of 60°C and pH 5. The cellulase enzyme was stable within the pH ranges of 3–5 and retained 67% of activity even at 55°C. Cellulase activity was found to be enhanced in the presence of metal ions such as Cd2+, Pb2+, and Ba2+. The enzyme showed the highest activity when CMC was used as a substrate, followed by cellobiose. The Km and Vmax values of the enzyme were 1.8 mg/ml and 10.92 μg/ml/min, respectively. The cellulase enzyme obtained from Bacillus licheniformis PANG L had suitable catalytic properties for use in industrial applications.http://dx.doi.org/10.1155/2023/3615757 |
| spellingShingle | Manita Shyaula Sunil Regmi Deegendra Khadka Ram Chandra Poudel Agni Dhakal Devesh Koirala Jaishree Sijapati Anjana Singh Jyoti Maharjan Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil International Journal of Microbiology |
| title | Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil |
| title_full | Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil |
| title_fullStr | Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil |
| title_full_unstemmed | Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil |
| title_short | Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil |
| title_sort | characterization of thermostable cellulase from bacillus licheniformis pang l isolated from the himalayan soil |
| url | http://dx.doi.org/10.1155/2023/3615757 |
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