Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil

This study aimed to isolate, purify, and characterize a potential thermophilic cellulase-producing bacterium from the Himalayan soil. Eleven thermophilic bacteria were isolated, and the strain PANG L was found to be the most potent cellulolytic producer. Morphological, physiological, biochemical, an...

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Main Authors: Manita Shyaula, Sunil Regmi, Deegendra Khadka, Ram Chandra Poudel, Agni Dhakal, Devesh Koirala, Jaishree Sijapati, Anjana Singh, Jyoti Maharjan
Format: Article
Language:English
Published: Wiley 2023-01-01
Series:International Journal of Microbiology
Online Access:http://dx.doi.org/10.1155/2023/3615757
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author Manita Shyaula
Sunil Regmi
Deegendra Khadka
Ram Chandra Poudel
Agni Dhakal
Devesh Koirala
Jaishree Sijapati
Anjana Singh
Jyoti Maharjan
author_facet Manita Shyaula
Sunil Regmi
Deegendra Khadka
Ram Chandra Poudel
Agni Dhakal
Devesh Koirala
Jaishree Sijapati
Anjana Singh
Jyoti Maharjan
author_sort Manita Shyaula
collection DOAJ
description This study aimed to isolate, purify, and characterize a potential thermophilic cellulase-producing bacterium from the Himalayan soil. Eleven thermophilic bacteria were isolated, and the strain PANG L was found to be the most potent cellulolytic producer. Morphological, physiological, biochemical, and molecular characterization identified PANG L as Bacillus licheniformis. This is the first study on the isolation of thermostable cellulase-producing Bacillus licheniformis from the Himalayan soil. This bacterium was processed for the production of cellulase enzyme. The optimum conditions for cellulase production were achieved at 45°C after 48 h of incubation at pH 6.5 in media-containing carboxymethyl cellulose (CMC) and yeast extract as carbon and nitrogen sources, respectively, in a thermo-shaker at 100 rpm. The enzyme was partially purified by 80% ammonium sulphate precipitation followed by dialysis, resulting in a 1.52-fold purification. The optimal activity of partially purified cellulase was observed at a temperature of 60°C and pH 5. The cellulase enzyme was stable within the pH ranges of 3–5 and retained 67% of activity even at 55°C. Cellulase activity was found to be enhanced in the presence of metal ions such as Cd2+, Pb2+, and Ba2+. The enzyme showed the highest activity when CMC was used as a substrate, followed by cellobiose. The Km and Vmax values of the enzyme were 1.8 mg/ml and 10.92 μg/ml/min, respectively. The cellulase enzyme obtained from Bacillus licheniformis PANG L had suitable catalytic properties for use in industrial applications.
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spelling doaj-art-da1b7da2aa1e4bbebca8b5e6aca4b9da2025-08-20T03:24:22ZengWileyInternational Journal of Microbiology1687-91982023-01-01202310.1155/2023/3615757Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan SoilManita Shyaula0Sunil Regmi1Deegendra Khadka2Ram Chandra Poudel3Agni Dhakal4Devesh Koirala5Jaishree Sijapati6Anjana Singh7Jyoti Maharjan8Central Department of MicrobiologyNepal Academy of Science and TechnologyNepal Academy of Science and TechnologyNepal Academy of Science and TechnologyNepal Academy of Science and TechnologyNepal Academy of Science and TechnologyNepal Academy of Science and TechnologyCentral Department of MicrobiologyNepal Academy of Science and TechnologyThis study aimed to isolate, purify, and characterize a potential thermophilic cellulase-producing bacterium from the Himalayan soil. Eleven thermophilic bacteria were isolated, and the strain PANG L was found to be the most potent cellulolytic producer. Morphological, physiological, biochemical, and molecular characterization identified PANG L as Bacillus licheniformis. This is the first study on the isolation of thermostable cellulase-producing Bacillus licheniformis from the Himalayan soil. This bacterium was processed for the production of cellulase enzyme. The optimum conditions for cellulase production were achieved at 45°C after 48 h of incubation at pH 6.5 in media-containing carboxymethyl cellulose (CMC) and yeast extract as carbon and nitrogen sources, respectively, in a thermo-shaker at 100 rpm. The enzyme was partially purified by 80% ammonium sulphate precipitation followed by dialysis, resulting in a 1.52-fold purification. The optimal activity of partially purified cellulase was observed at a temperature of 60°C and pH 5. The cellulase enzyme was stable within the pH ranges of 3–5 and retained 67% of activity even at 55°C. Cellulase activity was found to be enhanced in the presence of metal ions such as Cd2+, Pb2+, and Ba2+. The enzyme showed the highest activity when CMC was used as a substrate, followed by cellobiose. The Km and Vmax values of the enzyme were 1.8 mg/ml and 10.92 μg/ml/min, respectively. The cellulase enzyme obtained from Bacillus licheniformis PANG L had suitable catalytic properties for use in industrial applications.http://dx.doi.org/10.1155/2023/3615757
spellingShingle Manita Shyaula
Sunil Regmi
Deegendra Khadka
Ram Chandra Poudel
Agni Dhakal
Devesh Koirala
Jaishree Sijapati
Anjana Singh
Jyoti Maharjan
Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil
International Journal of Microbiology
title Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil
title_full Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil
title_fullStr Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil
title_full_unstemmed Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil
title_short Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil
title_sort characterization of thermostable cellulase from bacillus licheniformis pang l isolated from the himalayan soil
url http://dx.doi.org/10.1155/2023/3615757
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