NOX4 serves as a pan-cancer prognostic biomarker and therapeutic target in tumorigenesis
Abstract NADPH oxidase 4 (NOX4) is a key regulator of intracellular reactive oxygen species (ROS) and plays a critical role in tumorigenesis and cancer progression. It contributes to cancer cell transformation, proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT). To eluci...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-09690-9 |
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| author | Jiting Sun Zhiyuan Gong Lijie Zheng Zhixian Ding Lusheng Wang Yu Tang Shizhan Deng Yonggan Dong Heng Tang |
| author_facet | Jiting Sun Zhiyuan Gong Lijie Zheng Zhixian Ding Lusheng Wang Yu Tang Shizhan Deng Yonggan Dong Heng Tang |
| author_sort | Jiting Sun |
| collection | DOAJ |
| description | Abstract NADPH oxidase 4 (NOX4) is a key regulator of intracellular reactive oxygen species (ROS) and plays a critical role in tumorigenesis and cancer progression. It contributes to cancer cell transformation, proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT). To elucidate the molecular mechanisms underlying NOX4-mediated tumorigenesis, we performed a comprehensive pan-cancer bioinformatics analysis, integrating data from The Cancer Genome Atlas (TCGA), and validated our findings with in vitro experiments. We systematically analyzed NOX4 expression patterns across various cancer types and explored the correlations between NOX4 expression and patient survival, immune infiltration, tumor mutational burden (TMB), and microsatellite instability (MSI). In vitro assays, including Wound healing, Transwell, and CCK-8 assays, were conducted to validate the biological functions of NOX4 in breast cancer cells. Pan-cancer analysis revealed that NOX4 is significantly upregulated in various cancers, including breast cancer. Elevated NOX4 expression is associated with poor patient prognosis, immune cell infiltration, TMB, and MSI. Functional experiments confirmed that downregulation of NOX4 can inhibit the proliferation and metastasis of breast cancer cells. Our pan-cancer analysis provides valuable insights into the role of NOX4 in tumorigenesis. These results highlight NOX4 as a promising biomarker for prognosis and a potential therapeutic target for anti-tumor treatments across multiple cancer types. |
| format | Article |
| id | doaj-art-da0e807619264116ba4fc80e3b6ae46c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-da0e807619264116ba4fc80e3b6ae46c2025-08-20T04:02:45ZengNature PortfolioScientific Reports2045-23222025-07-0115111110.1038/s41598-025-09690-9NOX4 serves as a pan-cancer prognostic biomarker and therapeutic target in tumorigenesisJiting Sun0Zhiyuan Gong1Lijie Zheng2Zhixian Ding3Lusheng Wang4Yu Tang5Shizhan Deng6Yonggan Dong7Heng Tang8Central Laboratory, Wanbei Coal Electric Group General HospitalTianjin Medical UniversityCentral Laboratory, Wanbei Coal Electric Group General HospitalCentral Laboratory, Wanbei Coal Electric Group General HospitalCentral Laboratory, Wanbei Coal Electric Group General HospitalCentral Laboratory, Wanbei Coal Electric Group General HospitalCentral Laboratory, Wanbei Coal Electric Group General HospitalCentral Laboratory, Wanbei Coal Electric Group General HospitalCentral Laboratory, Wanbei Coal Electric Group General HospitalAbstract NADPH oxidase 4 (NOX4) is a key regulator of intracellular reactive oxygen species (ROS) and plays a critical role in tumorigenesis and cancer progression. It contributes to cancer cell transformation, proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT). To elucidate the molecular mechanisms underlying NOX4-mediated tumorigenesis, we performed a comprehensive pan-cancer bioinformatics analysis, integrating data from The Cancer Genome Atlas (TCGA), and validated our findings with in vitro experiments. We systematically analyzed NOX4 expression patterns across various cancer types and explored the correlations between NOX4 expression and patient survival, immune infiltration, tumor mutational burden (TMB), and microsatellite instability (MSI). In vitro assays, including Wound healing, Transwell, and CCK-8 assays, were conducted to validate the biological functions of NOX4 in breast cancer cells. Pan-cancer analysis revealed that NOX4 is significantly upregulated in various cancers, including breast cancer. Elevated NOX4 expression is associated with poor patient prognosis, immune cell infiltration, TMB, and MSI. Functional experiments confirmed that downregulation of NOX4 can inhibit the proliferation and metastasis of breast cancer cells. Our pan-cancer analysis provides valuable insights into the role of NOX4 in tumorigenesis. These results highlight NOX4 as a promising biomarker for prognosis and a potential therapeutic target for anti-tumor treatments across multiple cancer types.https://doi.org/10.1038/s41598-025-09690-9NADPH oxidase 4Pan-cancerPrognostic biomarkers |
| spellingShingle | Jiting Sun Zhiyuan Gong Lijie Zheng Zhixian Ding Lusheng Wang Yu Tang Shizhan Deng Yonggan Dong Heng Tang NOX4 serves as a pan-cancer prognostic biomarker and therapeutic target in tumorigenesis Scientific Reports NADPH oxidase 4 Pan-cancer Prognostic biomarkers |
| title | NOX4 serves as a pan-cancer prognostic biomarker and therapeutic target in tumorigenesis |
| title_full | NOX4 serves as a pan-cancer prognostic biomarker and therapeutic target in tumorigenesis |
| title_fullStr | NOX4 serves as a pan-cancer prognostic biomarker and therapeutic target in tumorigenesis |
| title_full_unstemmed | NOX4 serves as a pan-cancer prognostic biomarker and therapeutic target in tumorigenesis |
| title_short | NOX4 serves as a pan-cancer prognostic biomarker and therapeutic target in tumorigenesis |
| title_sort | nox4 serves as a pan cancer prognostic biomarker and therapeutic target in tumorigenesis |
| topic | NADPH oxidase 4 Pan-cancer Prognostic biomarkers |
| url | https://doi.org/10.1038/s41598-025-09690-9 |
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