Study on the Fingerprint Spectrum and the Spectrum-Effect Relationship of Analgesic and Anti-Inflammatory Effects of the Aqueous Extract from Dalbergia hancai Benth
Dalbergia hancai Benth. (D. hancai) is one of the most frequently utilized traditional Chinese medicine in Zhuang medicine. Simultaneously, it has been included in the “Quality Standard of Zhuang medicine in Guangxi Zhuang Autonomous Region (Vol. 2)” and possessed outstanding pharmacological effects...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2023/1242756 |
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| author | Qin Qiu Xiaofang Liu Chunying Huang Yanling Guo Dandan Zhen Junhao Shi Baojun Gu Hanshen Zhen Miao Zhang |
| author_facet | Qin Qiu Xiaofang Liu Chunying Huang Yanling Guo Dandan Zhen Junhao Shi Baojun Gu Hanshen Zhen Miao Zhang |
| author_sort | Qin Qiu |
| collection | DOAJ |
| description | Dalbergia hancai Benth. (D. hancai) is one of the most frequently utilized traditional Chinese medicine in Zhuang medicine. Simultaneously, it has been included in the “Quality Standard of Zhuang medicine in Guangxi Zhuang Autonomous Region (Vol. 2)” and possessed outstanding pharmacological effects. However, the pharmacodynamic material basis of D. hancai still remains unclear. In this study, the high-performance liquid chromatography (HPLC) method had been employed to establish the fingerprint of 10 batches of aqueous extract of D. hancai originated from different parts of China. At the same time, similarity evaluation, cluster analysis, and principal component analysis (PCA) had also been conducted to evaluate the common peaks. The acetic acid-induced writhing in mice had been employed as an analgesic model, and the carrageenan-induced toe swelling in mice was utilized as an anti-inflammatory model for pharmacodynamic experiments. The gray relational analysis (GRA) and partial least squares regression (PLSR) were applied to correlate the fingerprint and pharmacodynamic data to thoroughly examine its spectrum-effect relationship, whereby its analgesic and anti-inflammatory material basis had been comprehensively explored. The results revealed that the HPLC fingerprint of the aqueous extract of D. hancai had successfully identified 12 common peaks whereby two of which were further identified as protocatechuic acid and vitexin. Subsequently, through the analysis of GRA and PLSR, the chromatographic peaks that possess a critical correlation degree with the analgesic and anti-inflammatory effects of D. hancai had also been successfully discovered. Ultimately, the analgesic and anti-inflammatory effects of the 10 batches of D. hancai aqueous extract had been conclusively proved, and it was evidently indicated that these effects were attributable to the synergistic interactions between various components. Therefore, this study aims to serve as an effective analytical method for screening and predicting the effective substances of traditional Chinese medicine on the basis of the spectrum-effect relationship. |
| format | Article |
| id | doaj-art-da0a965402fb41d08b11743bbc6de93f |
| institution | Kabale University |
| issn | 2090-8873 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-da0a965402fb41d08b11743bbc6de93f2025-02-03T06:42:57ZengWileyJournal of Analytical Methods in Chemistry2090-88732023-01-01202310.1155/2023/1242756Study on the Fingerprint Spectrum and the Spectrum-Effect Relationship of Analgesic and Anti-Inflammatory Effects of the Aqueous Extract from Dalbergia hancai BenthQin Qiu0Xiaofang Liu1Chunying Huang2Yanling Guo3Dandan Zhen4Junhao Shi5Baojun Gu6Hanshen Zhen7Miao Zhang8Guangxi University of Chinese MedicalGuangxi University of Chinese MedicalTraditional Chinese Medicine Hospital of YuLinThe People’s Hospital of WuZhouGuangxi University of Chinese MedicalGuangxi University of Chinese MedicalGuangxi University of Chinese MedicalGuangxi University of Chinese MedicalGuangxi University of Chinese MedicalDalbergia hancai Benth. (D. hancai) is one of the most frequently utilized traditional Chinese medicine in Zhuang medicine. Simultaneously, it has been included in the “Quality Standard of Zhuang medicine in Guangxi Zhuang Autonomous Region (Vol. 2)” and possessed outstanding pharmacological effects. However, the pharmacodynamic material basis of D. hancai still remains unclear. In this study, the high-performance liquid chromatography (HPLC) method had been employed to establish the fingerprint of 10 batches of aqueous extract of D. hancai originated from different parts of China. At the same time, similarity evaluation, cluster analysis, and principal component analysis (PCA) had also been conducted to evaluate the common peaks. The acetic acid-induced writhing in mice had been employed as an analgesic model, and the carrageenan-induced toe swelling in mice was utilized as an anti-inflammatory model for pharmacodynamic experiments. The gray relational analysis (GRA) and partial least squares regression (PLSR) were applied to correlate the fingerprint and pharmacodynamic data to thoroughly examine its spectrum-effect relationship, whereby its analgesic and anti-inflammatory material basis had been comprehensively explored. The results revealed that the HPLC fingerprint of the aqueous extract of D. hancai had successfully identified 12 common peaks whereby two of which were further identified as protocatechuic acid and vitexin. Subsequently, through the analysis of GRA and PLSR, the chromatographic peaks that possess a critical correlation degree with the analgesic and anti-inflammatory effects of D. hancai had also been successfully discovered. Ultimately, the analgesic and anti-inflammatory effects of the 10 batches of D. hancai aqueous extract had been conclusively proved, and it was evidently indicated that these effects were attributable to the synergistic interactions between various components. Therefore, this study aims to serve as an effective analytical method for screening and predicting the effective substances of traditional Chinese medicine on the basis of the spectrum-effect relationship.http://dx.doi.org/10.1155/2023/1242756 |
| spellingShingle | Qin Qiu Xiaofang Liu Chunying Huang Yanling Guo Dandan Zhen Junhao Shi Baojun Gu Hanshen Zhen Miao Zhang Study on the Fingerprint Spectrum and the Spectrum-Effect Relationship of Analgesic and Anti-Inflammatory Effects of the Aqueous Extract from Dalbergia hancai Benth Journal of Analytical Methods in Chemistry |
| title | Study on the Fingerprint Spectrum and the Spectrum-Effect Relationship of Analgesic and Anti-Inflammatory Effects of the Aqueous Extract from Dalbergia hancai Benth |
| title_full | Study on the Fingerprint Spectrum and the Spectrum-Effect Relationship of Analgesic and Anti-Inflammatory Effects of the Aqueous Extract from Dalbergia hancai Benth |
| title_fullStr | Study on the Fingerprint Spectrum and the Spectrum-Effect Relationship of Analgesic and Anti-Inflammatory Effects of the Aqueous Extract from Dalbergia hancai Benth |
| title_full_unstemmed | Study on the Fingerprint Spectrum and the Spectrum-Effect Relationship of Analgesic and Anti-Inflammatory Effects of the Aqueous Extract from Dalbergia hancai Benth |
| title_short | Study on the Fingerprint Spectrum and the Spectrum-Effect Relationship of Analgesic and Anti-Inflammatory Effects of the Aqueous Extract from Dalbergia hancai Benth |
| title_sort | study on the fingerprint spectrum and the spectrum effect relationship of analgesic and anti inflammatory effects of the aqueous extract from dalbergia hancai benth |
| url | http://dx.doi.org/10.1155/2023/1242756 |
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