Simultaneous Determination of Stilbenes, Flavones, Coumestans, Anthraquinones, and Chalcones in Ethanolic Extract of Pet-Sang-Kard Mixed Herbal Remedy Using HPLC-PDA Analysis
The Pet-Sang-Kard mixed herbal remedy (PSKMHR) is a traditional Thai medicinal formulation used as an herbal supplement for the treatment of hemorrhoids. This remedy consists of four specific herbal ingredients in the following proportions: 50 parts <i>Cissus quadrangularis</i> L. stems,...
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2025-01-01
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| author | Weerasak Samee Wanna Eiamart Sarin Tadtong Chuda Chittasupho |
| author_facet | Weerasak Samee Wanna Eiamart Sarin Tadtong Chuda Chittasupho |
| author_sort | Weerasak Samee |
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| description | The Pet-Sang-Kard mixed herbal remedy (PSKMHR) is a traditional Thai medicinal formulation used as an herbal supplement for the treatment of hemorrhoids. This remedy consists of four specific herbal ingredients in the following proportions: 50 parts <i>Cissus quadrangularis</i> L. stems, 15 parts <i>Eclipta prostrata</i> L. aerial parts, 10 parts <i>Rheum</i> sp. rhizome, and 10 parts <i>Boesenbergia rotunda</i> (L.) Mansf. rhizome. This study presents the development, validation, and application of a high-performance liquid chromatography with photodiode array detection (HPLC-PDA) method designed for the simultaneous quantification of 13 key bioactive compounds, including rhaponticin, rhapontigenin, quercitrin, wedelolactone, aloe-emodin, rhein, emodin, chrysophanol, physcion, alpinetin, pinocembrin, pinostrobin, and panduratin A, present in the 70% ethanolic extract of PSKMHR. Method validation was conducted in accordance with Association of Official Analytical Collaboration (AOAC) international guidelines, evaluating parameters such as the specificity, linearity, accuracy, precision, and limit of detection. The results demonstrated exceptional linearity (R > 0.9999), high precision (% RSD < 2), and recovery rates within acceptable limits (98–102%) for all analytes. This developed method was successfully applied to quantify the 13 target compounds in the crude extracts of PSKMHR formulated from 10 market raw material samples, providing a robust analytical framework for quality control of this herbal remedy. |
| format | Article |
| id | doaj-art-d9ff61193fd940f5a742dcb68b1f78ed |
| institution | Kabale University |
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| publishDate | 2025-01-01 |
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| spelling | doaj-art-d9ff61193fd940f5a742dcb68b1f78ed2025-01-24T13:43:09ZengMDPI AGMolecules1420-30492025-01-0130222210.3390/molecules30020222Simultaneous Determination of Stilbenes, Flavones, Coumestans, Anthraquinones, and Chalcones in Ethanolic Extract of Pet-Sang-Kard Mixed Herbal Remedy Using HPLC-PDA AnalysisWeerasak Samee0Wanna Eiamart1Sarin Tadtong2Chuda Chittasupho3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok 26120, ThailandChula Pharmacokinetic Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Pharmacognosy, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok 26120, ThailandDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandThe Pet-Sang-Kard mixed herbal remedy (PSKMHR) is a traditional Thai medicinal formulation used as an herbal supplement for the treatment of hemorrhoids. This remedy consists of four specific herbal ingredients in the following proportions: 50 parts <i>Cissus quadrangularis</i> L. stems, 15 parts <i>Eclipta prostrata</i> L. aerial parts, 10 parts <i>Rheum</i> sp. rhizome, and 10 parts <i>Boesenbergia rotunda</i> (L.) Mansf. rhizome. This study presents the development, validation, and application of a high-performance liquid chromatography with photodiode array detection (HPLC-PDA) method designed for the simultaneous quantification of 13 key bioactive compounds, including rhaponticin, rhapontigenin, quercitrin, wedelolactone, aloe-emodin, rhein, emodin, chrysophanol, physcion, alpinetin, pinocembrin, pinostrobin, and panduratin A, present in the 70% ethanolic extract of PSKMHR. Method validation was conducted in accordance with Association of Official Analytical Collaboration (AOAC) international guidelines, evaluating parameters such as the specificity, linearity, accuracy, precision, and limit of detection. The results demonstrated exceptional linearity (R > 0.9999), high precision (% RSD < 2), and recovery rates within acceptable limits (98–102%) for all analytes. This developed method was successfully applied to quantify the 13 target compounds in the crude extracts of PSKMHR formulated from 10 market raw material samples, providing a robust analytical framework for quality control of this herbal remedy.https://www.mdpi.com/1420-3049/30/2/222stilbenesflavonescoumestansanthraquinoneschalconeschromatography |
| spellingShingle | Weerasak Samee Wanna Eiamart Sarin Tadtong Chuda Chittasupho Simultaneous Determination of Stilbenes, Flavones, Coumestans, Anthraquinones, and Chalcones in Ethanolic Extract of Pet-Sang-Kard Mixed Herbal Remedy Using HPLC-PDA Analysis Molecules stilbenes flavones coumestans anthraquinones chalcones chromatography |
| title | Simultaneous Determination of Stilbenes, Flavones, Coumestans, Anthraquinones, and Chalcones in Ethanolic Extract of Pet-Sang-Kard Mixed Herbal Remedy Using HPLC-PDA Analysis |
| title_full | Simultaneous Determination of Stilbenes, Flavones, Coumestans, Anthraquinones, and Chalcones in Ethanolic Extract of Pet-Sang-Kard Mixed Herbal Remedy Using HPLC-PDA Analysis |
| title_fullStr | Simultaneous Determination of Stilbenes, Flavones, Coumestans, Anthraquinones, and Chalcones in Ethanolic Extract of Pet-Sang-Kard Mixed Herbal Remedy Using HPLC-PDA Analysis |
| title_full_unstemmed | Simultaneous Determination of Stilbenes, Flavones, Coumestans, Anthraquinones, and Chalcones in Ethanolic Extract of Pet-Sang-Kard Mixed Herbal Remedy Using HPLC-PDA Analysis |
| title_short | Simultaneous Determination of Stilbenes, Flavones, Coumestans, Anthraquinones, and Chalcones in Ethanolic Extract of Pet-Sang-Kard Mixed Herbal Remedy Using HPLC-PDA Analysis |
| title_sort | simultaneous determination of stilbenes flavones coumestans anthraquinones and chalcones in ethanolic extract of pet sang kard mixed herbal remedy using hplc pda analysis |
| topic | stilbenes flavones coumestans anthraquinones chalcones chromatography |
| url | https://www.mdpi.com/1420-3049/30/2/222 |
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