Differential Antimicrobial Potential of Ajuga integrifolia Buch. Ham. Ex D.Don Based on Extraction Solvents
The current study aimed to evaluate the effect of extraction solvents on the anti-bacterial and anti-fungal efficacy of the stem and roots of Ajuga integrifolia Buch. Ham. ex D. Extracts were prepared in different solvents and tested against fungi and bacteria species including, Agrobacterium tumefa...
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2025-01-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | https://agrifoodscience.com/index.php/TURJAF/article/view/5770 |
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| author | Wajid Khan Nisar Ali Muhammad Nazir Uddin Dawood Ghafoor Muhammad Ishaq Haji Khan Muhammad Rizwan Shahab Khan |
| author_facet | Wajid Khan Nisar Ali Muhammad Nazir Uddin Dawood Ghafoor Muhammad Ishaq Haji Khan Muhammad Rizwan Shahab Khan |
| author_sort | Wajid Khan |
| collection | DOAJ |
| description | The current study aimed to evaluate the effect of extraction solvents on the anti-bacterial and anti-fungal efficacy of the stem and roots of Ajuga integrifolia Buch. Ham. ex D. Extracts were prepared in different solvents and tested against fungi and bacteria species including, Agrobacterium tumefaciens, Xanthomonos oryzae, Citrobacter freundi, Alternaria alternata, A. solani and Aspergillus niger. Antibacterial efficacy of the Ajuga integrifolia was carried out by disc diffusion susceptibility method and antifungal efficacy by well diffusion susceptibility method. Methanol stem extract revealed efficacy against C. freundi by producing a 63% zone of inhibition at 3000µg/disc while methanol roots extract produced 77% ZI against A. tumifaciens. Methanol root and stem extracts produced an equal zone of inhibition (97%) at 1000 µg/ml concentration against A. alternata and A. solani. The results of the study clearly stated that the polarity of the solvents used in the extraction procedure affects the bioactivities of the extracts. |
| format | Article |
| id | doaj-art-d9eff73c819a424688959737fa90659d |
| institution | Kabale University |
| issn | 2148-127X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-d9eff73c819a424688959737fa90659d2025-02-06T13:44:58ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2025-01-01131869110.24925/turjaf.v13i1.86-91.57704519Differential Antimicrobial Potential of Ajuga integrifolia Buch. Ham. Ex D.Don Based on Extraction SolventsWajid Khan0https://orcid.org/0000-0003-4463-3750Nisar Ali1https://orcid.org/0009-0001-9610-7253Muhammad Nazir Uddin2https://orcid.org/0000-0002-2339-1062Dawood Ghafoor3https://orcid.org/0009-0000-8140-8275Muhammad Ishaq4https://orcid.org/0009-0004-3481-0869Haji Khan5https://orcid.org/0000-0002-3263-306XMuhammad Rizwan6https://orcid.org/0000-0001-5266-4982Shahab Khan7https://orcid.org/0000-0001-7022-9394Center for Biotechnology and Microbiology, University of Swat, PakistanCenter for Biotechnology and Microbiology, University of Swat, PakistanCenter for Biotechnology and Microbiology, University of Swat, PakistanCenter for Biotechnology and Microbiology, University of Swat, PakistanCenter for Biotechnology and Microbiology, University of Swat, PakistanCenter for Biotechnology and Microbiology, University of Swat, PakistanCenter for Biotechnology and Microbiology, University of Swat, PakistanCenter for Biotechnology and Microbiology, University of Swat, PakistanThe current study aimed to evaluate the effect of extraction solvents on the anti-bacterial and anti-fungal efficacy of the stem and roots of Ajuga integrifolia Buch. Ham. ex D. Extracts were prepared in different solvents and tested against fungi and bacteria species including, Agrobacterium tumefaciens, Xanthomonos oryzae, Citrobacter freundi, Alternaria alternata, A. solani and Aspergillus niger. Antibacterial efficacy of the Ajuga integrifolia was carried out by disc diffusion susceptibility method and antifungal efficacy by well diffusion susceptibility method. Methanol stem extract revealed efficacy against C. freundi by producing a 63% zone of inhibition at 3000µg/disc while methanol roots extract produced 77% ZI against A. tumifaciens. Methanol root and stem extracts produced an equal zone of inhibition (97%) at 1000 µg/ml concentration against A. alternata and A. solani. The results of the study clearly stated that the polarity of the solvents used in the extraction procedure affects the bioactivities of the extracts.https://agrifoodscience.com/index.php/TURJAF/article/view/5770extraction solventantibacterial efficacyantifungal activitydisc diffusion assaycrude extract |
| spellingShingle | Wajid Khan Nisar Ali Muhammad Nazir Uddin Dawood Ghafoor Muhammad Ishaq Haji Khan Muhammad Rizwan Shahab Khan Differential Antimicrobial Potential of Ajuga integrifolia Buch. Ham. Ex D.Don Based on Extraction Solvents Turkish Journal of Agriculture: Food Science and Technology extraction solvent antibacterial efficacy antifungal activity disc diffusion assay crude extract |
| title | Differential Antimicrobial Potential of Ajuga integrifolia Buch. Ham. Ex D.Don Based on Extraction Solvents |
| title_full | Differential Antimicrobial Potential of Ajuga integrifolia Buch. Ham. Ex D.Don Based on Extraction Solvents |
| title_fullStr | Differential Antimicrobial Potential of Ajuga integrifolia Buch. Ham. Ex D.Don Based on Extraction Solvents |
| title_full_unstemmed | Differential Antimicrobial Potential of Ajuga integrifolia Buch. Ham. Ex D.Don Based on Extraction Solvents |
| title_short | Differential Antimicrobial Potential of Ajuga integrifolia Buch. Ham. Ex D.Don Based on Extraction Solvents |
| title_sort | differential antimicrobial potential of ajuga integrifolia buch ham ex d don based on extraction solvents |
| topic | extraction solvent antibacterial efficacy antifungal activity disc diffusion assay crude extract |
| url | https://agrifoodscience.com/index.php/TURJAF/article/view/5770 |
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