NADP-Dependent Isocitrate Dehydrogenase from Arabidopsis Roots Contributes in the Mechanism of Defence against the Nitro-Oxidative Stress Induced by Salinity
NADPH regeneration appears to be essential in the mechanism of plant defence against oxidative stress. Plants contain several NADPH-generating dehydrogenases including isocitrate dehydrogenase (NADP-ICDH), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), and malic...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/694740 |
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| author | Marina Leterrier Juan B. Barroso Raquel Valderrama José M. Palma Francisco J. Corpas |
| author_facet | Marina Leterrier Juan B. Barroso Raquel Valderrama José M. Palma Francisco J. Corpas |
| author_sort | Marina Leterrier |
| collection | DOAJ |
| description | NADPH regeneration appears to be essential in the mechanism of plant defence against oxidative stress. Plants contain several NADPH-generating dehydrogenases including isocitrate dehydrogenase (NADP-ICDH), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), and malic enzyme (ME). In Arabidopsis seedlings grown under salinity conditions (100 mM NaCl) the analysis of physiological parameters, antioxidant enzymes (catalase and superoxide dismutase) and content of superoxide radical (O2 ∙−), nitric oxide (NO), and peroxynitrite (ONOO-) indicates a process of nitro-oxidative stress induced by NaCl. Among the analysed NADPH-generating dehydrogenases under salinity conditions, the NADP-ICDH showed the maximum activity mainly attributable to the root NADP-ICDH. Thus, these data provide new insights on the relevance of the NADP-ICDH which could be considered as a second barrier in the mechanism of response against the nitro-oxidative stress generated by salinity. |
| format | Article |
| id | doaj-art-d9ba746500be4c45ac2500ae226c075f |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-d9ba746500be4c45ac2500ae226c075f2025-02-03T01:23:46ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/694740694740NADP-Dependent Isocitrate Dehydrogenase from Arabidopsis Roots Contributes in the Mechanism of Defence against the Nitro-Oxidative Stress Induced by SalinityMarina Leterrier0Juan B. Barroso1Raquel Valderrama2José M. Palma3Francisco J. Corpas4Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apartado 419, 18080 Granada, SpainGrupo de Señalización Molecular y Sistemas Antioxidantes en Plantas, Unidad Asociada al CSIC (EEZ), Departamento de Bioquímica y Biología Molecular, Universidad de Jaén, 23071 Jaén, SpainGrupo de Señalización Molecular y Sistemas Antioxidantes en Plantas, Unidad Asociada al CSIC (EEZ), Departamento de Bioquímica y Biología Molecular, Universidad de Jaén, 23071 Jaén, SpainDepartamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apartado 419, 18080 Granada, SpainDepartamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apartado 419, 18080 Granada, SpainNADPH regeneration appears to be essential in the mechanism of plant defence against oxidative stress. Plants contain several NADPH-generating dehydrogenases including isocitrate dehydrogenase (NADP-ICDH), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), and malic enzyme (ME). In Arabidopsis seedlings grown under salinity conditions (100 mM NaCl) the analysis of physiological parameters, antioxidant enzymes (catalase and superoxide dismutase) and content of superoxide radical (O2 ∙−), nitric oxide (NO), and peroxynitrite (ONOO-) indicates a process of nitro-oxidative stress induced by NaCl. Among the analysed NADPH-generating dehydrogenases under salinity conditions, the NADP-ICDH showed the maximum activity mainly attributable to the root NADP-ICDH. Thus, these data provide new insights on the relevance of the NADP-ICDH which could be considered as a second barrier in the mechanism of response against the nitro-oxidative stress generated by salinity.http://dx.doi.org/10.1100/2012/694740 |
| spellingShingle | Marina Leterrier Juan B. Barroso Raquel Valderrama José M. Palma Francisco J. Corpas NADP-Dependent Isocitrate Dehydrogenase from Arabidopsis Roots Contributes in the Mechanism of Defence against the Nitro-Oxidative Stress Induced by Salinity The Scientific World Journal |
| title | NADP-Dependent Isocitrate Dehydrogenase from Arabidopsis Roots Contributes in the Mechanism of Defence against the Nitro-Oxidative Stress Induced by Salinity |
| title_full | NADP-Dependent Isocitrate Dehydrogenase from Arabidopsis Roots Contributes in the Mechanism of Defence against the Nitro-Oxidative Stress Induced by Salinity |
| title_fullStr | NADP-Dependent Isocitrate Dehydrogenase from Arabidopsis Roots Contributes in the Mechanism of Defence against the Nitro-Oxidative Stress Induced by Salinity |
| title_full_unstemmed | NADP-Dependent Isocitrate Dehydrogenase from Arabidopsis Roots Contributes in the Mechanism of Defence against the Nitro-Oxidative Stress Induced by Salinity |
| title_short | NADP-Dependent Isocitrate Dehydrogenase from Arabidopsis Roots Contributes in the Mechanism of Defence against the Nitro-Oxidative Stress Induced by Salinity |
| title_sort | nadp dependent isocitrate dehydrogenase from arabidopsis roots contributes in the mechanism of defence against the nitro oxidative stress induced by salinity |
| url | http://dx.doi.org/10.1100/2012/694740 |
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