Evaluation of Bacterial Load and Antibiotic Resistance Pattern of Staphylococcus aureus from Ready-to-Eat Raw Beef in Bahir Dar City, Ethiopia
Background. Staphylococcus aureus is one of the most important causes of food-borne intoxication and the most frequent antibiotic-resistant pathogen in the world. Regular evaluation of the current safety status of food is a proactive measure to minimize the possible danger of food-borne pathogens. T...
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| Format: | Article |
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Wiley
2021-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2021/5560596 |
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| author | Bizuneh Tsehayneh Taddesse Yayeh Birhan Agmas |
| author_facet | Bizuneh Tsehayneh Taddesse Yayeh Birhan Agmas |
| author_sort | Bizuneh Tsehayneh |
| collection | DOAJ |
| description | Background. Staphylococcus aureus is one of the most important causes of food-borne intoxication and the most frequent antibiotic-resistant pathogen in the world. Regular evaluation of the current safety status of food is a proactive measure to minimize the possible danger of food-borne pathogens. Therefore, this study was conducted to assess the bacterial load and antibiotic resistance profile of S. aureus from ready-to-eat raw beef in Bahir Dar city, Ethiopia. Methodology. This cross-sectional study was conducted from October 2018 to April 2019 by collecting a total of 101 raw beef samples from butcher shops using a simple random sampling method. Isolation and microbial load determination of S. aureus use were performed by conventional culture method and an antibiotic susceptibility test was conducted by using Kirby Bauer disk diffusion method on the Mueller–Hinton agar. The data were analyzed by using STATA software version 12.0. Result. Out of 101 raw beef samples, 55 (54.45%) were positive for S. aureus with a mean bacterial count of 3.40 ± 0.63 (log10 cfu/g). About 13% of butcher shops had unacceptable and potentially dangerous (above 104 cfu/g) bacterial load. High S. aureus drug resistance was observed on penicillin (92.73%) followed by cefoxitin (74.5%), tetracycline (63.63%), and clindamycin (50.9%). On the other hand, there was the highest susceptibility for ciprofloxacin (100%) followed by gentamycin (90.91%) and erythromycin (87.27%). Multidrug resistance was also found in 54 (98%) of the isolates. Conclusion. In this study highly drug-resistant S. aureus was incriminated as the main meat contaminant in butcheries of Bahir Dar city. Therefore, appropriate antimicrobial use and staphylococcal control methods should be employed to prevent S. aureus intoxications in foods. |
| format | Article |
| id | doaj-art-d98732d1299c40de8341a700fb38333b |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-d98732d1299c40de8341a700fb38333b2025-02-03T06:43:32ZengWileyInternational Journal of Microbiology1687-918X1687-91982021-01-01202110.1155/2021/55605965560596Evaluation of Bacterial Load and Antibiotic Resistance Pattern of Staphylococcus aureus from Ready-to-Eat Raw Beef in Bahir Dar City, EthiopiaBizuneh Tsehayneh0Taddesse Yayeh1Birhan Agmas2Department of Veterinary Science, College of Agriculture and Environmental Sciences, Bahir Dar University, Bahir Dar, EthiopiaDepartment of Veterinary Science, College of Agriculture and Environmental Sciences, Bahir Dar University, Bahir Dar, EthiopiaDepartment of Veterinary Science, College of Agriculture and Environmental Sciences, Bahir Dar University, Bahir Dar, EthiopiaBackground. Staphylococcus aureus is one of the most important causes of food-borne intoxication and the most frequent antibiotic-resistant pathogen in the world. Regular evaluation of the current safety status of food is a proactive measure to minimize the possible danger of food-borne pathogens. Therefore, this study was conducted to assess the bacterial load and antibiotic resistance profile of S. aureus from ready-to-eat raw beef in Bahir Dar city, Ethiopia. Methodology. This cross-sectional study was conducted from October 2018 to April 2019 by collecting a total of 101 raw beef samples from butcher shops using a simple random sampling method. Isolation and microbial load determination of S. aureus use were performed by conventional culture method and an antibiotic susceptibility test was conducted by using Kirby Bauer disk diffusion method on the Mueller–Hinton agar. The data were analyzed by using STATA software version 12.0. Result. Out of 101 raw beef samples, 55 (54.45%) were positive for S. aureus with a mean bacterial count of 3.40 ± 0.63 (log10 cfu/g). About 13% of butcher shops had unacceptable and potentially dangerous (above 104 cfu/g) bacterial load. High S. aureus drug resistance was observed on penicillin (92.73%) followed by cefoxitin (74.5%), tetracycline (63.63%), and clindamycin (50.9%). On the other hand, there was the highest susceptibility for ciprofloxacin (100%) followed by gentamycin (90.91%) and erythromycin (87.27%). Multidrug resistance was also found in 54 (98%) of the isolates. Conclusion. In this study highly drug-resistant S. aureus was incriminated as the main meat contaminant in butcheries of Bahir Dar city. Therefore, appropriate antimicrobial use and staphylococcal control methods should be employed to prevent S. aureus intoxications in foods.http://dx.doi.org/10.1155/2021/5560596 |
| spellingShingle | Bizuneh Tsehayneh Taddesse Yayeh Birhan Agmas Evaluation of Bacterial Load and Antibiotic Resistance Pattern of Staphylococcus aureus from Ready-to-Eat Raw Beef in Bahir Dar City, Ethiopia International Journal of Microbiology |
| title | Evaluation of Bacterial Load and Antibiotic Resistance Pattern of Staphylococcus aureus from Ready-to-Eat Raw Beef in Bahir Dar City, Ethiopia |
| title_full | Evaluation of Bacterial Load and Antibiotic Resistance Pattern of Staphylococcus aureus from Ready-to-Eat Raw Beef in Bahir Dar City, Ethiopia |
| title_fullStr | Evaluation of Bacterial Load and Antibiotic Resistance Pattern of Staphylococcus aureus from Ready-to-Eat Raw Beef in Bahir Dar City, Ethiopia |
| title_full_unstemmed | Evaluation of Bacterial Load and Antibiotic Resistance Pattern of Staphylococcus aureus from Ready-to-Eat Raw Beef in Bahir Dar City, Ethiopia |
| title_short | Evaluation of Bacterial Load and Antibiotic Resistance Pattern of Staphylococcus aureus from Ready-to-Eat Raw Beef in Bahir Dar City, Ethiopia |
| title_sort | evaluation of bacterial load and antibiotic resistance pattern of staphylococcus aureus from ready to eat raw beef in bahir dar city ethiopia |
| url | http://dx.doi.org/10.1155/2021/5560596 |
| work_keys_str_mv | AT bizunehtsehayneh evaluationofbacterialloadandantibioticresistancepatternofstaphylococcusaureusfromreadytoeatrawbeefinbahirdarcityethiopia AT taddesseyayeh evaluationofbacterialloadandantibioticresistancepatternofstaphylococcusaureusfromreadytoeatrawbeefinbahirdarcityethiopia AT birhanagmas evaluationofbacterialloadandantibioticresistancepatternofstaphylococcusaureusfromreadytoeatrawbeefinbahirdarcityethiopia |