Identification of candidate pheromones and neurotransmitter regulation of agonistic behavior in Macrobrachium rosenbergii
Macrobrachium rosenbergii, a crucial commercial prawn species in China, exhibits agonistic behavior under high-density culture conditions, which negatively impacts their integrity, survival, and growth, causing economic losses. This study utilized liquid chromatography mass spectrometry (LC-MS) to i...
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Elsevier
2025-09-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425003060 |
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| author | Hangyu Qi Qi Shen Jiongying Yu Miaoying Cai Jianyu Song Junjie Wu Guoliang Yang Shaokui Yi |
| author_facet | Hangyu Qi Qi Shen Jiongying Yu Miaoying Cai Jianyu Song Junjie Wu Guoliang Yang Shaokui Yi |
| author_sort | Hangyu Qi |
| collection | DOAJ |
| description | Macrobrachium rosenbergii, a crucial commercial prawn species in China, exhibits agonistic behavior under high-density culture conditions, which negatively impacts their integrity, survival, and growth, causing economic losses. This study utilized liquid chromatography mass spectrometry (LC-MS) to identify and classify the metabolic differences in urine and water mixtures of M. rosenbergii displaying agonistic behavior versus those without. Seventeen compounds, such as 4-Acetamidobutyric acid, 1-oleoyl-2-myristoyl-sn-glycero-3-phosphocholine, N-Acety-L-valine, 11-Dehydrocorticosterone, Sphinganine (Sph t18:0), and Acitretin, were identified as candidate pheromones associated with agonistic behavior, 4-Acetamidobutyric acid and Sph (t18:0) were hypothesized to be particularly significant. Additionally, the roles of serotonin (5-HT) and dopamine (DA) in agonistic behavior among M. rosenbergii males were evaluated. We found that 5-HT levels in hemolymph significantly decreased after fighting (P < 0.05), while DA levels significantly increased (P < 0.05) compared to the control group. The receptor genes of 5-HT and DA (5-HTRs and DARs) were further analyzed. Gene expression analyses of M. rosenbergii before and after agonistic behavior revealed that Mr5-HTR1 exhibited significant changes in hemolymph and abdominal ganglia, while Mr5-HTR2 varied across five tissues, and MrDAR1 and MrDAR2 showed significant changes in the brain and abdominal ganglia. To verify the impact of 5-HTRs on the agonistic behavior, 5-HTRs agonist and antagonist were in vivo injected. The results showed that the injection of 5-HTRs agonists significantly inhibited the occurrence of agonistic behavior in M. rosenbergii. This study deepens our understanding of the regulatory mechanisms of crustacean agonistic behavior and provides a theoretical basis for improving the crustacean farming management. |
| format | Article |
| id | doaj-art-d91f0c8ff0414eb79a9baf34a0d81403 |
| institution | DOAJ |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-d91f0c8ff0414eb79a9baf34a0d814032025-08-20T02:57:08ZengElsevierAquaculture Reports2352-51342025-09-014310292010.1016/j.aqrep.2025.102920Identification of candidate pheromones and neurotransmitter regulation of agonistic behavior in Macrobrachium rosenbergiiHangyu Qi0Qi Shen1Jiongying Yu2Miaoying Cai3Jianyu Song4Junjie Wu5Guoliang Yang6Shaokui Yi7School of Life Sciences, Huzhou University, Huzhou 313000, ChinaSchool of Life Sciences, Huzhou University, Huzhou 313000, ChinaSchool of Life Sciences, Huzhou University, Huzhou 313000, ChinaJiangsu Shufeng Prawn Breeding Co. LTD., Gaoyou 225654, ChinaYunnan Academy of Fishery Sciences, Kunming 650224, ChinaYunnan Academy of Fishery Sciences, Kunming 650224, ChinaSchool of Life Sciences, Huzhou University, Huzhou 313000, China; Jiangsu Shufeng Prawn Breeding Co. LTD., Gaoyou 225654, ChinaSchool of Life Sciences, Huzhou University, Huzhou 313000, China; Corresponding author.Macrobrachium rosenbergii, a crucial commercial prawn species in China, exhibits agonistic behavior under high-density culture conditions, which negatively impacts their integrity, survival, and growth, causing economic losses. This study utilized liquid chromatography mass spectrometry (LC-MS) to identify and classify the metabolic differences in urine and water mixtures of M. rosenbergii displaying agonistic behavior versus those without. Seventeen compounds, such as 4-Acetamidobutyric acid, 1-oleoyl-2-myristoyl-sn-glycero-3-phosphocholine, N-Acety-L-valine, 11-Dehydrocorticosterone, Sphinganine (Sph t18:0), and Acitretin, were identified as candidate pheromones associated with agonistic behavior, 4-Acetamidobutyric acid and Sph (t18:0) were hypothesized to be particularly significant. Additionally, the roles of serotonin (5-HT) and dopamine (DA) in agonistic behavior among M. rosenbergii males were evaluated. We found that 5-HT levels in hemolymph significantly decreased after fighting (P < 0.05), while DA levels significantly increased (P < 0.05) compared to the control group. The receptor genes of 5-HT and DA (5-HTRs and DARs) were further analyzed. Gene expression analyses of M. rosenbergii before and after agonistic behavior revealed that Mr5-HTR1 exhibited significant changes in hemolymph and abdominal ganglia, while Mr5-HTR2 varied across five tissues, and MrDAR1 and MrDAR2 showed significant changes in the brain and abdominal ganglia. To verify the impact of 5-HTRs on the agonistic behavior, 5-HTRs agonist and antagonist were in vivo injected. The results showed that the injection of 5-HTRs agonists significantly inhibited the occurrence of agonistic behavior in M. rosenbergii. This study deepens our understanding of the regulatory mechanisms of crustacean agonistic behavior and provides a theoretical basis for improving the crustacean farming management.http://www.sciencedirect.com/science/article/pii/S2352513425003060Macrobrachium rosenbergiiAgonistic behaviorPheromone5-HTDA |
| spellingShingle | Hangyu Qi Qi Shen Jiongying Yu Miaoying Cai Jianyu Song Junjie Wu Guoliang Yang Shaokui Yi Identification of candidate pheromones and neurotransmitter regulation of agonistic behavior in Macrobrachium rosenbergii Aquaculture Reports Macrobrachium rosenbergii Agonistic behavior Pheromone 5-HT DA |
| title | Identification of candidate pheromones and neurotransmitter regulation of agonistic behavior in Macrobrachium rosenbergii |
| title_full | Identification of candidate pheromones and neurotransmitter regulation of agonistic behavior in Macrobrachium rosenbergii |
| title_fullStr | Identification of candidate pheromones and neurotransmitter regulation of agonistic behavior in Macrobrachium rosenbergii |
| title_full_unstemmed | Identification of candidate pheromones and neurotransmitter regulation of agonistic behavior in Macrobrachium rosenbergii |
| title_short | Identification of candidate pheromones and neurotransmitter regulation of agonistic behavior in Macrobrachium rosenbergii |
| title_sort | identification of candidate pheromones and neurotransmitter regulation of agonistic behavior in macrobrachium rosenbergii |
| topic | Macrobrachium rosenbergii Agonistic behavior Pheromone 5-HT DA |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425003060 |
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