Simultaneous Determination of Lamivudine, Zidovudine and Abacavir in Tablet Dosage Forms by RP HPLC Method
A simple, accurate and reproducible RP-HPLC method has been developed for the simultaneous determination of lamivudine, zidovudine and abacavir in tablet dosage forms. Chromatography was carried out on a HiQ Sil C 18 V column using a mobile phase consisting of 0.01 M potassium dihydrogen ortho-phosp...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2010/473798 |
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| author | D. Anantha Kumar G. Srinivasa Rao J. V. L. N. Seshagiri Rao |
| author_facet | D. Anantha Kumar G. Srinivasa Rao J. V. L. N. Seshagiri Rao |
| author_sort | D. Anantha Kumar |
| collection | DOAJ |
| description | A simple, accurate and reproducible RP-HPLC method has been developed for the simultaneous determination of lamivudine, zidovudine and abacavir in tablet dosage forms. Chromatography was carried out on a HiQ Sil C 18 V column using a mobile phase consisting of 0.01 M potassium dihydrogen ortho-phosphate (pH 3.0) and methanol (55:45 v/v) at a flow rate of 0.8 mL/min. The detection was made at 272 nm and stavudine was used as the internal standard for this study. The retention times for lamivudine, abacavir and zidovudine were found to be 3.8, 6.3, 8.1 min. respectively. The calibration curves were linear over the range 5-250 μg/mL for both zidovudine and abacavir and 5-140 μg/mL for lamivudine. The proposed method was validated as per ICH and USP guidelines and it was found suitable for the routine quality control analysis of the drugs in tablet dosage forms. |
| format | Article |
| id | doaj-art-d907d28ddc5146d49adf3749c0636796 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-d907d28ddc5146d49adf3749c06367962025-02-03T05:53:54ZengWileyE-Journal of Chemistry0973-49452090-98102010-01-017118018410.1155/2010/473798Simultaneous Determination of Lamivudine, Zidovudine and Abacavir in Tablet Dosage Forms by RP HPLC MethodD. Anantha Kumar0G. Srinivasa Rao1J. V. L. N. Seshagiri Rao2Pharmaceutical Analysis and Quality Assurance Division, A. U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530 003, IndiaPharmaceutical Analysis and Quality Assurance Division, A. U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530 003, IndiaPharmaceutical Analysis and Quality Assurance Division, A. U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530 003, IndiaA simple, accurate and reproducible RP-HPLC method has been developed for the simultaneous determination of lamivudine, zidovudine and abacavir in tablet dosage forms. Chromatography was carried out on a HiQ Sil C 18 V column using a mobile phase consisting of 0.01 M potassium dihydrogen ortho-phosphate (pH 3.0) and methanol (55:45 v/v) at a flow rate of 0.8 mL/min. The detection was made at 272 nm and stavudine was used as the internal standard for this study. The retention times for lamivudine, abacavir and zidovudine were found to be 3.8, 6.3, 8.1 min. respectively. The calibration curves were linear over the range 5-250 μg/mL for both zidovudine and abacavir and 5-140 μg/mL for lamivudine. The proposed method was validated as per ICH and USP guidelines and it was found suitable for the routine quality control analysis of the drugs in tablet dosage forms.http://dx.doi.org/10.1155/2010/473798 |
| spellingShingle | D. Anantha Kumar G. Srinivasa Rao J. V. L. N. Seshagiri Rao Simultaneous Determination of Lamivudine, Zidovudine and Abacavir in Tablet Dosage Forms by RP HPLC Method E-Journal of Chemistry |
| title | Simultaneous Determination of Lamivudine, Zidovudine and Abacavir in Tablet Dosage Forms by RP HPLC Method |
| title_full | Simultaneous Determination of Lamivudine, Zidovudine and Abacavir in Tablet Dosage Forms by RP HPLC Method |
| title_fullStr | Simultaneous Determination of Lamivudine, Zidovudine and Abacavir in Tablet Dosage Forms by RP HPLC Method |
| title_full_unstemmed | Simultaneous Determination of Lamivudine, Zidovudine and Abacavir in Tablet Dosage Forms by RP HPLC Method |
| title_short | Simultaneous Determination of Lamivudine, Zidovudine and Abacavir in Tablet Dosage Forms by RP HPLC Method |
| title_sort | simultaneous determination of lamivudine zidovudine and abacavir in tablet dosage forms by rp hplc method |
| url | http://dx.doi.org/10.1155/2010/473798 |
| work_keys_str_mv | AT dananthakumar simultaneousdeterminationoflamivudinezidovudineandabacavirintabletdosageformsbyrphplcmethod AT gsrinivasarao simultaneousdeterminationoflamivudinezidovudineandabacavirintabletdosageformsbyrphplcmethod AT jvlnseshagirirao simultaneousdeterminationoflamivudinezidovudineandabacavirintabletdosageformsbyrphplcmethod |