Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang”
In order to breed virus-free plantlets of the kiwifruit cultivar “Guichang,” which belongs to Actinidia deliciosa, in this study, stem segments with buds were used as explants, the establishment of a tissue culture rapid propagation system was carried out, and then the virus status of tissue culture...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/9951949 |
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| author | Weimin Zhong Junliang Zhou Dongmei Tang Yaxin Huang Futao Liu Min Zhang Guoli Wang Sufang Wu Yue He Jingwen Tang |
| author_facet | Weimin Zhong Junliang Zhou Dongmei Tang Yaxin Huang Futao Liu Min Zhang Guoli Wang Sufang Wu Yue He Jingwen Tang |
| author_sort | Weimin Zhong |
| collection | DOAJ |
| description | In order to breed virus-free plantlets of the kiwifruit cultivar “Guichang,” which belongs to Actinidia deliciosa, in this study, stem segments with buds were used as explants, the establishment of a tissue culture rapid propagation system was carried out, and then the virus status of tissue culture plantlets was detected via the real-time reverse transcription-polymerase chain reaction (RT-qPCR) method. The tissue culture rapid propagation system proved that the contamination and browning rates could be controlled below 20% and the survival rate could be exceeded by 70% when the single bud stem segment of “Guichang” kiwifruit was sterilized with 70% alcohol for 30–60 s and 15% NaClO for 15 min, respectively. Meanwhile, we screened the hormone concentration to get better results, and the appropriate medium for adventitious bud induction was MS + 6-BA (1.0 mg/L) + IBA (0.2 mg/L); for proliferation, it was MS + 6-BA (1.0 mg/L) + IBA (0.1 mg/L); and for rooting, it was 1/2 MS + IBA (0.3 mg/L), and the efficiency of induction, proliferation, and rooting could reach 74.07%, 79.63%, and 85.18%, respectively. In addition, the RT-qPCR results demonstrated that the infection rate of 9 viruses: apple stem grooving virus (ASGV), cucumber mosaic virus (CMV), Actinidia virus X (AVX), cucumber necrosis virus (CNV), ribgrass mosaic virus (RMV), citrus leaf blotch virus (CLBV), Actinidia virus B (AcVB), Pelargonium zonate spot virus (PZSV), and cherry leaf roll virus (CLRV) in the “Guichang” kiwifruit tissue culture plantlets was 0. This study could lay a foundation for the production of “Guichang” kiwifruit tissue culture seedlings, and the medium formula provided in this study was useful for the industrial rapid propagation of “Guichang” plantlets. |
| format | Article |
| id | doaj-art-d8ecc2dc20c14ebdaf027ca15b53f401 |
| institution | Kabale University |
| issn | 2090-9071 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-d8ecc2dc20c14ebdaf027ca15b53f4012025-02-03T07:24:09ZengWileyJournal of Chemistry2090-90712021-01-01202110.1155/2021/9951949Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang”Weimin Zhong0Junliang Zhou1Dongmei Tang2Yaxin Huang3Futao Liu4Min Zhang5Guoli Wang6Sufang Wu7Yue He8Jingwen Tang9Institute of Fruit ScienceInstitute of Fruit ScienceInstitute of Fruit ScienceAgricultural Bureau of Xiuwen CountyInstitute of Fruit ScienceInstitute of Fruit ScienceAgricultural Bureau of Xiuwen CountyAgricultural Bureau of Xiuwen CountyAgricultural Bureau of Xiuwen CountyAgricultural Bureau of Xiuwen CountyIn order to breed virus-free plantlets of the kiwifruit cultivar “Guichang,” which belongs to Actinidia deliciosa, in this study, stem segments with buds were used as explants, the establishment of a tissue culture rapid propagation system was carried out, and then the virus status of tissue culture plantlets was detected via the real-time reverse transcription-polymerase chain reaction (RT-qPCR) method. The tissue culture rapid propagation system proved that the contamination and browning rates could be controlled below 20% and the survival rate could be exceeded by 70% when the single bud stem segment of “Guichang” kiwifruit was sterilized with 70% alcohol for 30–60 s and 15% NaClO for 15 min, respectively. Meanwhile, we screened the hormone concentration to get better results, and the appropriate medium for adventitious bud induction was MS + 6-BA (1.0 mg/L) + IBA (0.2 mg/L); for proliferation, it was MS + 6-BA (1.0 mg/L) + IBA (0.1 mg/L); and for rooting, it was 1/2 MS + IBA (0.3 mg/L), and the efficiency of induction, proliferation, and rooting could reach 74.07%, 79.63%, and 85.18%, respectively. In addition, the RT-qPCR results demonstrated that the infection rate of 9 viruses: apple stem grooving virus (ASGV), cucumber mosaic virus (CMV), Actinidia virus X (AVX), cucumber necrosis virus (CNV), ribgrass mosaic virus (RMV), citrus leaf blotch virus (CLBV), Actinidia virus B (AcVB), Pelargonium zonate spot virus (PZSV), and cherry leaf roll virus (CLRV) in the “Guichang” kiwifruit tissue culture plantlets was 0. This study could lay a foundation for the production of “Guichang” kiwifruit tissue culture seedlings, and the medium formula provided in this study was useful for the industrial rapid propagation of “Guichang” plantlets.http://dx.doi.org/10.1155/2021/9951949 |
| spellingShingle | Weimin Zhong Junliang Zhou Dongmei Tang Yaxin Huang Futao Liu Min Zhang Guoli Wang Sufang Wu Yue He Jingwen Tang Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang” Journal of Chemistry |
| title | Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang” |
| title_full | Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang” |
| title_fullStr | Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang” |
| title_full_unstemmed | Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang” |
| title_short | Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang” |
| title_sort | establishment of tissue culture system of actinidia deliciosa cultivar guichang |
| url | http://dx.doi.org/10.1155/2021/9951949 |
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