Applicability of Next-generation Sequencing Techniques in Assessing Non-alcoholic Fatty Liver Disease: A Comprehensive Review
Non-alcoholic Fatty Liver Disease (NAFLD) is a burgeoning global health concern, with a spectrum of severity ranging from simple steatosis to Non-alcoholic Steatohepatitis (NASH). While liver biopsy remains the gold standard for diagnosis, the need for non invasive alternatives has spurred interest...
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JCDR Research and Publications Private Limited
2025-06-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/21048/77924_CE[Ra1]_F(IS)_QC(PS_OM)_PF1(JY_SS)_PFA(OM)_PN(IS).pdf |
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| author | Ali Mahzari |
| author_facet | Ali Mahzari |
| author_sort | Ali Mahzari |
| collection | DOAJ |
| description | Non-alcoholic Fatty Liver Disease (NAFLD) is a burgeoning global health concern, with a spectrum of severity ranging from simple steatosis to Non-alcoholic Steatohepatitis (NASH). While liver biopsy remains the gold standard for diagnosis, the need for non invasive alternatives has spurred interest in Next-generation Sequencing (NGS) technologies. NGS technologies enable the simultaneous sequencing of millions of Deoxyribonucleic Acid (DNA) or Ribonucleic Acid (RNA) fragments, providing a high-throughput approach to analyse genetic variations, gene expression and epigenetic modifications. A comprehensive literature search was conducted to identify studies investigating the use of NGS in NAFLD. Data extraction focused on NGS techniques, study design, key findings and clinical implications. NGS has demonstrated potential in unraveling the complex genetic and molecular underpinnings of NAFLD. The identification of genetic variants, epigenetic modifications and non coding RNA alterations has advanced our understanding of disease pathogenesis. Moreover, NGS-based approaches have shown promise in differentiating NAFLD subtypes and predicting disease progression. However, challenges related to data analysis, standardisation and clinical translation persist. NGS offers a promising avenue for improving NAFLD diagnosis, prognosis and management. While significant advancements have been made, further research is needed to fully realise its clinical potential. |
| format | Article |
| id | doaj-art-d8d5dbe3ca9a445e9cebe9f8dae6f9ba |
| institution | DOAJ |
| issn | 2249-782X 0973-709X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj-art-d8d5dbe3ca9a445e9cebe9f8dae6f9ba2025-08-20T03:19:25ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2025-06-01196OE01OE0510.7860/JCDR/2025/77924.21048Applicability of Next-generation Sequencing Techniques in Assessing Non-alcoholic Fatty Liver Disease: A Comprehensive ReviewAli Mahzari0Associate Professor, Department of Laboratory Medicine, Al-Baha University, Riyadh, Saudi Arabia.Non-alcoholic Fatty Liver Disease (NAFLD) is a burgeoning global health concern, with a spectrum of severity ranging from simple steatosis to Non-alcoholic Steatohepatitis (NASH). While liver biopsy remains the gold standard for diagnosis, the need for non invasive alternatives has spurred interest in Next-generation Sequencing (NGS) technologies. NGS technologies enable the simultaneous sequencing of millions of Deoxyribonucleic Acid (DNA) or Ribonucleic Acid (RNA) fragments, providing a high-throughput approach to analyse genetic variations, gene expression and epigenetic modifications. A comprehensive literature search was conducted to identify studies investigating the use of NGS in NAFLD. Data extraction focused on NGS techniques, study design, key findings and clinical implications. NGS has demonstrated potential in unraveling the complex genetic and molecular underpinnings of NAFLD. The identification of genetic variants, epigenetic modifications and non coding RNA alterations has advanced our understanding of disease pathogenesis. Moreover, NGS-based approaches have shown promise in differentiating NAFLD subtypes and predicting disease progression. However, challenges related to data analysis, standardisation and clinical translation persist. NGS offers a promising avenue for improving NAFLD diagnosis, prognosis and management. While significant advancements have been made, further research is needed to fully realise its clinical potential.https://jcdr.net/articles/PDF/21048/77924_CE[Ra1]_F(IS)_QC(PS_OM)_PF1(JY_SS)_PFA(OM)_PN(IS).pdfdiagnosisepigeneticsgeneticsgene expressionnon invasiveomicspathogenesissurveillance |
| spellingShingle | Ali Mahzari Applicability of Next-generation Sequencing Techniques in Assessing Non-alcoholic Fatty Liver Disease: A Comprehensive Review Journal of Clinical and Diagnostic Research diagnosis epigenetics genetics gene expression non invasive omics pathogenesis surveillance |
| title | Applicability of Next-generation Sequencing Techniques in Assessing Non-alcoholic Fatty Liver Disease: A Comprehensive Review |
| title_full | Applicability of Next-generation Sequencing Techniques in Assessing Non-alcoholic Fatty Liver Disease: A Comprehensive Review |
| title_fullStr | Applicability of Next-generation Sequencing Techniques in Assessing Non-alcoholic Fatty Liver Disease: A Comprehensive Review |
| title_full_unstemmed | Applicability of Next-generation Sequencing Techniques in Assessing Non-alcoholic Fatty Liver Disease: A Comprehensive Review |
| title_short | Applicability of Next-generation Sequencing Techniques in Assessing Non-alcoholic Fatty Liver Disease: A Comprehensive Review |
| title_sort | applicability of next generation sequencing techniques in assessing non alcoholic fatty liver disease a comprehensive review |
| topic | diagnosis epigenetics genetics gene expression non invasive omics pathogenesis surveillance |
| url | https://jcdr.net/articles/PDF/21048/77924_CE[Ra1]_F(IS)_QC(PS_OM)_PF1(JY_SS)_PFA(OM)_PN(IS).pdf |
| work_keys_str_mv | AT alimahzari applicabilityofnextgenerationsequencingtechniquesinassessingnonalcoholicfattyliverdiseaseacomprehensivereview |