Strain-limited biofilm regulation through the Brg1-Rme1 circuit in Candida albicans
ABSTRACT Prominent virulence traits of Candida albicans include its ability to produce filamentous hyphal cells and grow as a biofilm. These traits are under control of numerous transcription factors (TFs), including Brg1 and Rme1. In the reference strain SC5314, a brg1Δ/Δ mutant has reduced levels...
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American Society for Microbiology
2025-01-01
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| Series: | mSphere |
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| Online Access: | https://journals.asm.org/doi/10.1128/msphere.00980-24 |
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| author | Min-Ju Kim Aaron P. Mitchell |
| author_facet | Min-Ju Kim Aaron P. Mitchell |
| author_sort | Min-Ju Kim |
| collection | DOAJ |
| description | ABSTRACT Prominent virulence traits of Candida albicans include its ability to produce filamentous hyphal cells and grow as a biofilm. These traits are under control of numerous transcription factors (TFs), including Brg1 and Rme1. In the reference strain SC5314, a brg1Δ/Δ mutant has reduced levels of biofilm/filament production; a brg1Δ/Δ rme1Δ/Δ double mutant has wild-type levels of biofilm/filament production. Here, we asked whether this suppression relationship is preserved in four additional strain backgrounds: P76067, P57055, P87, and P75010. These strains represent diverse clades and biofilm/filament production abilities. We find that a rme1Δ/Δ mutation restores biofilm/filament production in a brg1Δ/Δ mutant of P76067, but not in brg1Δ/Δ mutants of P57055, P87, and P75010. We speculate that variation in activities of two functionally related TFs, Nrg1, and Ume6, may cause the strain-limited impact of the rme1Δ/Δ mutation.IMPORTANCECandida albicans is a widespread fungal pathogen. The regulatory circuitry underlying virulence traits is well studied in the reference strain background, but not in other clinical isolate backgrounds. Here, we describe a pronounced example of strain variation in the control of two prominent virulence traits, biofilm formation and filamentation. |
| format | Article |
| id | doaj-art-d8d51b18fb6e4fa9b522e36bfe0f6a06 |
| institution | Kabale University |
| issn | 2379-5042 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj-art-d8d51b18fb6e4fa9b522e36bfe0f6a062025-01-28T14:00:57ZengAmerican Society for MicrobiologymSphere2379-50422025-01-0110110.1128/msphere.00980-24Strain-limited biofilm regulation through the Brg1-Rme1 circuit in Candida albicansMin-Ju Kim0Aaron P. Mitchell1Department of Microbiology, University of Georgia, Athens, Georgia, USADepartment of Microbiology, University of Georgia, Athens, Georgia, USAABSTRACT Prominent virulence traits of Candida albicans include its ability to produce filamentous hyphal cells and grow as a biofilm. These traits are under control of numerous transcription factors (TFs), including Brg1 and Rme1. In the reference strain SC5314, a brg1Δ/Δ mutant has reduced levels of biofilm/filament production; a brg1Δ/Δ rme1Δ/Δ double mutant has wild-type levels of biofilm/filament production. Here, we asked whether this suppression relationship is preserved in four additional strain backgrounds: P76067, P57055, P87, and P75010. These strains represent diverse clades and biofilm/filament production abilities. We find that a rme1Δ/Δ mutation restores biofilm/filament production in a brg1Δ/Δ mutant of P76067, but not in brg1Δ/Δ mutants of P57055, P87, and P75010. We speculate that variation in activities of two functionally related TFs, Nrg1, and Ume6, may cause the strain-limited impact of the rme1Δ/Δ mutation.IMPORTANCECandida albicans is a widespread fungal pathogen. The regulatory circuitry underlying virulence traits is well studied in the reference strain background, but not in other clinical isolate backgrounds. Here, we describe a pronounced example of strain variation in the control of two prominent virulence traits, biofilm formation and filamentation.https://journals.asm.org/doi/10.1128/msphere.00980-24Candida albicansstrain variationhypha formationbiofilm |
| spellingShingle | Min-Ju Kim Aaron P. Mitchell Strain-limited biofilm regulation through the Brg1-Rme1 circuit in Candida albicans mSphere Candida albicans strain variation hypha formation biofilm |
| title | Strain-limited biofilm regulation through the Brg1-Rme1 circuit in Candida albicans |
| title_full | Strain-limited biofilm regulation through the Brg1-Rme1 circuit in Candida albicans |
| title_fullStr | Strain-limited biofilm regulation through the Brg1-Rme1 circuit in Candida albicans |
| title_full_unstemmed | Strain-limited biofilm regulation through the Brg1-Rme1 circuit in Candida albicans |
| title_short | Strain-limited biofilm regulation through the Brg1-Rme1 circuit in Candida albicans |
| title_sort | strain limited biofilm regulation through the brg1 rme1 circuit in candida albicans |
| topic | Candida albicans strain variation hypha formation biofilm |
| url | https://journals.asm.org/doi/10.1128/msphere.00980-24 |
| work_keys_str_mv | AT minjukim strainlimitedbiofilmregulationthroughthebrg1rme1circuitincandidaalbicans AT aaronpmitchell strainlimitedbiofilmregulationthroughthebrg1rme1circuitincandidaalbicans |