The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001
Estrogen contamination is recognized as one of the most serious environmental problems, causing widespread concern worldwide. Environmental estrogens are mainly derived from human and vertebrate excretion, drugs, and agricultural activities. The use of microorganisms is currently the most economical...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2020/9369182 |
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| author | Kejian Tian Fanxing Meng Qi Meng Yan Gao Lili Zhang Le Wang Yuqing Wang Xue Li Hongliang Huo |
| author_facet | Kejian Tian Fanxing Meng Qi Meng Yan Gao Lili Zhang Le Wang Yuqing Wang Xue Li Hongliang Huo |
| author_sort | Kejian Tian |
| collection | DOAJ |
| description | Estrogen contamination is recognized as one of the most serious environmental problems, causing widespread concern worldwide. Environmental estrogens are mainly derived from human and vertebrate excretion, drugs, and agricultural activities. The use of microorganisms is currently the most economical and effective method for biodegradation of environmental estrogens. Rhodococcus equi DSSKP-R-001 (R-001) has strong estrogen-degrading capabilities. Our study indicated that R-001 can use different types of estrogen as its sole carbon source for growth and metabolism, with final degradation rates above 90%. Transcriptome analysis showed that 720 (E1), 983 (E2), and 845 (EE2) genes were significantly upregulated in the estrogen-treated group compared with the control group, and 270 differentially expressed genes (DEGs) were upregulated across all treatment groups. These DEGs included ABC transporters; estrogen-degrading genes, including those that perform initial oxidation and dehydrogenation reactions and those that further degrade the resulting substrates into small molecules; and metabolism genes that complete the intracellular transformation and utilization of estrogen metabolites through biological processes such as amino acid metabolism, lipid metabolism, carbohydrate metabolism, and the tricarboxylic acid cycle. In summary, the biodegradation of estrogens is coordinated by a metabolic network of estrogen-degrading enzymes, transporters, metabolic enzymes, and other coenzymes. In this study, the metabolic mechanisms by which Rhodococcus equi R-001 degrades various estrogens were analyzed for the first time. A new pollutant metabolism system is outlined, providing a starting point for the construction of engineered estrogen-degrading bacteria. |
| format | Article |
| id | doaj-art-d8d49b37e2fa4be5b2a9b70f3d985194 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
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| series | International Journal of Genomics |
| spelling | doaj-art-d8d49b37e2fa4be5b2a9b70f3d9851942025-02-03T06:45:48ZengWileyInternational Journal of Genomics2314-436X2314-43782020-01-01202010.1155/2020/93691829369182The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001Kejian Tian0Fanxing Meng1Qi Meng2Yan Gao3Lili Zhang4Le Wang5Yuqing Wang6Xue Li7Hongliang Huo8School of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, ChinaSchool of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, ChinaSchool of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, ChinaSchool of Life Sciences, Northeast Normal University, No. 5268, Renmin Main Street, Nanguan District, Changchun City, Jilin Province, ChinaSchool of Life Sciences, Northeast Normal University, No. 5268, Renmin Main Street, Nanguan District, Changchun City, Jilin Province, ChinaSchool of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, ChinaSchool of Life Sciences, Northeast Normal University, No. 5268, Renmin Main Street, Nanguan District, Changchun City, Jilin Province, ChinaSchool of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, ChinaSchool of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, ChinaEstrogen contamination is recognized as one of the most serious environmental problems, causing widespread concern worldwide. Environmental estrogens are mainly derived from human and vertebrate excretion, drugs, and agricultural activities. The use of microorganisms is currently the most economical and effective method for biodegradation of environmental estrogens. Rhodococcus equi DSSKP-R-001 (R-001) has strong estrogen-degrading capabilities. Our study indicated that R-001 can use different types of estrogen as its sole carbon source for growth and metabolism, with final degradation rates above 90%. Transcriptome analysis showed that 720 (E1), 983 (E2), and 845 (EE2) genes were significantly upregulated in the estrogen-treated group compared with the control group, and 270 differentially expressed genes (DEGs) were upregulated across all treatment groups. These DEGs included ABC transporters; estrogen-degrading genes, including those that perform initial oxidation and dehydrogenation reactions and those that further degrade the resulting substrates into small molecules; and metabolism genes that complete the intracellular transformation and utilization of estrogen metabolites through biological processes such as amino acid metabolism, lipid metabolism, carbohydrate metabolism, and the tricarboxylic acid cycle. In summary, the biodegradation of estrogens is coordinated by a metabolic network of estrogen-degrading enzymes, transporters, metabolic enzymes, and other coenzymes. In this study, the metabolic mechanisms by which Rhodococcus equi R-001 degrades various estrogens were analyzed for the first time. A new pollutant metabolism system is outlined, providing a starting point for the construction of engineered estrogen-degrading bacteria.http://dx.doi.org/10.1155/2020/9369182 |
| spellingShingle | Kejian Tian Fanxing Meng Qi Meng Yan Gao Lili Zhang Le Wang Yuqing Wang Xue Li Hongliang Huo The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001 International Journal of Genomics |
| title | The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001 |
| title_full | The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001 |
| title_fullStr | The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001 |
| title_full_unstemmed | The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001 |
| title_short | The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001 |
| title_sort | analysis of estrogen degrading and functional metabolism genes in rhodococcus equi dsskp r 001 |
| url | http://dx.doi.org/10.1155/2020/9369182 |
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